学科主题临床医学
Comparisons for detecting NY-ESO-1 mRNA expression levels in hepatocellular carcinoma tissues
Wang, Xue-Yan; Chen, Hong-Song; Luo, Su; Zhang, Heng-Hui; Fei, Ran; Cai, Jun
关键词NY-ESO-1 mRNA expression hepatocellular carcinoma
刊名ONCOLOGY REPORTS
2009-03-01
DOI10.3892/or_00000276
21期:3页:713-719
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Oncology
研究领域[WOS]Oncology
关键词[WOS]TESTIS ANTIGEN NY-ESO-1 ; CANCER/TESTIS CT ANTIGENS ; IMMUNE-RESPONSES ; CANCER-PATIENTS ; GENE ; MELANOMA ; DEFINITION ; SARCOMA ; LINE
英文摘要

NY-ESO-1 is a cancer/testis (CT) antigen expressed in normal adult tissues solely in the testicular germ cells of normal adults and in various cancers. It induces specific humoral and cellular immunity in patients with NY-ESO-1-expressing cancer. We compared the expression of NY-ESO-1 mRNA in hepatocellular carcinoma (HCC) patients by using various primers and DNA polymerases to optimize RT-PCR conditions and to evaluate the correlations among the expression levels of NY-ESO-1, LAGE-1 and SSX-1 and clinical parameters. We determined differences in the abilities of the various primers and DNA polymerases to amplify the NY-ESO-1 gene at different exons. Primers designated as P3 detected targeted sequences better than primers P1 and P2; AmpliTaq Gold (R) DNA polymerase was more effective than Platinum (R) pfx DNA polymerase and Taq DNA polymerase. NY-ESO-1, LAGE-1 and SSX-1 mRNAs were detected in 29.7, 45.3 and 37.5%, respectively, of the 64 HCC specimens. No CT antigen mRNAs were detected in the 64-paired adjacent non-cancerous tissues. The frequency for the co-expression of one, two or three antigens of NY-ESO-1, LAGE-1 and SSX-1 was 57.8, 35.9 and 18.8%, respectively. We also analyzed the relationships among the CT antigen expression levels and several clinical parameters. There were no significant differences between CT antigen expression levels and clinical parameters, except the correlations between the expression of SSX-1 and the age of the patients.

语种英语
WOS记录号WOS:000263584000019
项目编号30772499 ; 985-2-099-113 ; 2008ZX 10002-019
资助机构National Natural Science Foundation of China ; Program for New Century Excellent Talents ; National Key Sci-Tcch Special Project of China
引用统计
被引频次:8[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/67050
专题北京大学第二临床医学院_北京大学肝病研究所
作者单位Peking Univ, Inst Hepatol, Peoples Hosp, Beijing 100044, Peoples R China
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Wang, Xue-Yan,Chen, Hong-Song,Luo, Su,et al. Comparisons for detecting NY-ESO-1 mRNA expression levels in hepatocellular carcinoma tissues[J]. ONCOLOGY REPORTS,2009,21(3):713-719.
APA Wang, Xue-Yan,Chen, Hong-Song,Luo, Su,Zhang, Heng-Hui,Fei, Ran,&Cai, Jun.(2009).Comparisons for detecting NY-ESO-1 mRNA expression levels in hepatocellular carcinoma tissues.ONCOLOGY REPORTS,21(3),713-719.
MLA Wang, Xue-Yan,et al."Comparisons for detecting NY-ESO-1 mRNA expression levels in hepatocellular carcinoma tissues".ONCOLOGY REPORTS 21.3(2009):713-719.
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