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学科主题: 临床医学
题名:
Comparisons for detecting NY-ESO-1 mRNA expression levels in hepatocellular carcinoma tissues
作者: Wang, Xue-Yan; Chen, Hong-Song; Luo, Su; Zhang, Heng-Hui; Fei, Ran; Cai, Jun
关键词: NY-ESO-1 ; mRNA expression ; hepatocellular carcinoma
刊名: ONCOLOGY REPORTS
发表日期: 2009-03-01
DOI: 10.3892/or_00000276
卷: 21, 期:3, 页:713-719
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Oncology
研究领域[WOS]: Oncology
关键词[WOS]: TESTIS ANTIGEN NY-ESO-1 ; CANCER/TESTIS CT ANTIGENS ; IMMUNE-RESPONSES ; CANCER-PATIENTS ; GENE ; MELANOMA ; DEFINITION ; SARCOMA ; LINE
英文摘要:

NY-ESO-1 is a cancer/testis (CT) antigen expressed in normal adult tissues solely in the testicular germ cells of normal adults and in various cancers. It induces specific humoral and cellular immunity in patients with NY-ESO-1-expressing cancer. We compared the expression of NY-ESO-1 mRNA in hepatocellular carcinoma (HCC) patients by using various primers and DNA polymerases to optimize RT-PCR conditions and to evaluate the correlations among the expression levels of NY-ESO-1, LAGE-1 and SSX-1 and clinical parameters. We determined differences in the abilities of the various primers and DNA polymerases to amplify the NY-ESO-1 gene at different exons. Primers designated as P3 detected targeted sequences better than primers P1 and P2; AmpliTaq Gold (R) DNA polymerase was more effective than Platinum (R) pfx DNA polymerase and Taq DNA polymerase. NY-ESO-1, LAGE-1 and SSX-1 mRNAs were detected in 29.7, 45.3 and 37.5%, respectively, of the 64 HCC specimens. No CT antigen mRNAs were detected in the 64-paired adjacent non-cancerous tissues. The frequency for the co-expression of one, two or three antigens of NY-ESO-1, LAGE-1 and SSX-1 was 57.8, 35.9 and 18.8%, respectively. We also analyzed the relationships among the CT antigen expression levels and several clinical parameters. There were no significant differences between CT antigen expression levels and clinical parameters, except the correlations between the expression of SSX-1 and the age of the patients.

语种: 英语
所属项目编号: 30772499 ; 985-2-099-113 ; 2008ZX 10002-019
项目资助者: National Natural Science Foundation of China ; Program for New Century Excellent Talents ; National Key Sci-Tcch Special Project of China
WOS记录号: WOS:000263584000019
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/67050
Appears in Collections:北京大学第二临床医学院_北京大学肝病研究所_期刊论文

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作者单位: Peking Univ, Inst Hepatol, Peoples Hosp, Beijing 100044, Peoples R China

Recommended Citation:
Wang, Xue-Yan,Chen, Hong-Song,Luo, Su,et al. Comparisons for detecting NY-ESO-1 mRNA expression levels in hepatocellular carcinoma tissues[J]. ONCOLOGY REPORTS,2009,21(3):713-719.
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