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学科主题: 临床医学
题名:
Icarisid II, a PDE5 inhibitor from Epimedium wanshanense, increases cellular cGMP by enhancing NOS in diabetic ED rats corpus cavernosum tissue
作者: Zhang, J.1,3; Wang, Y. -B.2; Ma, C. -G.1; Liu, T.3; Li, W. -R.3; Gong, Y. -Q.3; Xin, Z. -C.3
关键词: cGMP ; erectile dysfunction ; Icarisid II ; NOS ; PDE5 inhibitor
刊名: ANDROLOGIA
发表日期: 2012-05-01
DOI: 10.1111/j.1439-0272.2010.01144.x
卷: suppl.1, 页:87-93
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Andrology
研究领域[WOS]: Endocrinology & Metabolism
关键词[WOS]: ACTIVITY IN-VITRO ; PHOSPHODIESTERASE-5 ; CELLS ; DIFFERENTIATION ; EXTRACT
英文摘要:

The aim of this study was to explore the effects and mechanisms of Icarisid II (ICA-II) on enhancing the cellular cGMP in rat corpus cavernosum tissue (RCCT). Diabetes mellitus Wistar rats were induced by streptozotocin, and diabetic ED rats were selected for the RCCT culture by apomorphine. ICA-II was extracted and purified from Icariin (ICA) by enzymatic method. The RCCT was treated with ICA-II, ICA and Sildenafil at different concentrations. cGMP and nitric oxide synthase (NOS) activities were checked respectively by enzyme immunoassay. Meanwhile, nNOS, iNOS and eNOS in RCCT were checked by western blot. ICA-II evaluated the intracellular cGMP to 8.01 +/- 1.02 pmol mg-1 min-1, which is much weaker than that from Sildenafil (12.4 +/- 1.16 pmol mg-1 min-1) (P < 0.05). There is no significant difference between ICA-II and ICA. With the treatment of 10 mu m ICA-II for 24 and 48 h, nNOS expression was significantly increased in RCCT (P < 0.05), while the eNOS expression level was very low without any change. Notably, ICA-II increased the intracellular NOS activity significantly in vitro in RCCT. Except the PDE5 inhibitory effect, ICA-II increases the intracellular cGMP through the enhancement of nNOS expression and NOS activity in RCCT in vitro. ICA-II implies a potential compound for neurogenic erectile dysfunction by NOcGMP pathway.

语种: 英语
所属项目编号: 30670818 ; 30772285
项目资助者: Chinese Government ; Medical Research Foundation of Peking University (China)
WOS记录号: WOS:000304439300011
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/67055
Appears in Collections:北京大学第一临床医学院_男科中心_期刊论文

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作者单位: 1.Peking Univ, Hosp 1, Androl Ctr, Beijing 100871, Peoples R China
2.Capital Med Univ, Luhe Hosp, Dept Urol, Beijing 101149, Peoples R China
3.Peking Univ, Stomatol Hosp, Stomatol Clin 3, Beijing 100871, Peoples R China

Recommended Citation:
Zhang, J.,Wang, Y. -B.,Ma, C. -G.,et al. Icarisid II, a PDE5 inhibitor from Epimedium wanshanense, increases cellular cGMP by enhancing NOS in diabetic ED rats corpus cavernosum tissue[J]. ANDROLOGIA,2012,suppl.1:87-93.
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