IR@PKUHSC  > 北京大学第一临床医学院  > 男科中心
学科主题临床医学
Icarisid II, a PDE5 inhibitor from Epimedium wanshanense, increases cellular cGMP by enhancing NOS in diabetic ED rats corpus cavernosum tissue
Zhang, J.1,3; Wang, Y. -B.2; Ma, C. -G.1; Liu, T.3; Li, W. -R.3; Gong, Y. -Q.3; Xin, Z. -C.3
关键词cGMP erectile dysfunction Icarisid II NOS PDE5 inhibitor
刊名ANDROLOGIA
2012-05-01
DOI10.1111/j.1439-0272.2010.01144.x
suppl.1页:87-93
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Andrology
研究领域[WOS]Endocrinology & Metabolism
关键词[WOS]ACTIVITY IN-VITRO ; PHOSPHODIESTERASE-5 ; CELLS ; DIFFERENTIATION ; EXTRACT
英文摘要

The aim of this study was to explore the effects and mechanisms of Icarisid II (ICA-II) on enhancing the cellular cGMP in rat corpus cavernosum tissue (RCCT). Diabetes mellitus Wistar rats were induced by streptozotocin, and diabetic ED rats were selected for the RCCT culture by apomorphine. ICA-II was extracted and purified from Icariin (ICA) by enzymatic method. The RCCT was treated with ICA-II, ICA and Sildenafil at different concentrations. cGMP and nitric oxide synthase (NOS) activities were checked respectively by enzyme immunoassay. Meanwhile, nNOS, iNOS and eNOS in RCCT were checked by western blot. ICA-II evaluated the intracellular cGMP to 8.01 +/- 1.02 pmol mg-1 min-1, which is much weaker than that from Sildenafil (12.4 +/- 1.16 pmol mg-1 min-1) (P < 0.05). There is no significant difference between ICA-II and ICA. With the treatment of 10 mu m ICA-II for 24 and 48 h, nNOS expression was significantly increased in RCCT (P < 0.05), while the eNOS expression level was very low without any change. Notably, ICA-II increased the intracellular NOS activity significantly in vitro in RCCT. Except the PDE5 inhibitory effect, ICA-II increases the intracellular cGMP through the enhancement of nNOS expression and NOS activity in RCCT in vitro. ICA-II implies a potential compound for neurogenic erectile dysfunction by NOcGMP pathway.

语种英语
WOS记录号WOS:000304439300011
项目编号30670818 ; 30772285
资助机构Chinese Government ; Medical Research Foundation of Peking University (China)
引用统计
被引频次:17[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/67055
专题北京大学第一临床医学院_男科中心
作者单位1.Peking Univ, Hosp 1, Androl Ctr, Beijing 100871, Peoples R China
2.Capital Med Univ, Luhe Hosp, Dept Urol, Beijing 101149, Peoples R China
3.Peking Univ, Stomatol Hosp, Stomatol Clin 3, Beijing 100871, Peoples R China
推荐引用方式
GB/T 7714
Zhang, J.,Wang, Y. -B.,Ma, C. -G.,et al. Icarisid II, a PDE5 inhibitor from Epimedium wanshanense, increases cellular cGMP by enhancing NOS in diabetic ED rats corpus cavernosum tissue[J]. ANDROLOGIA,2012,suppl.1:87-93.
APA Zhang, J..,Wang, Y. -B..,Ma, C. -G..,Liu, T..,Li, W. -R..,...&Xin, Z. -C..(2012).Icarisid II, a PDE5 inhibitor from Epimedium wanshanense, increases cellular cGMP by enhancing NOS in diabetic ED rats corpus cavernosum tissue.ANDROLOGIA,suppl.1,87-93.
MLA Zhang, J.,et al."Icarisid II, a PDE5 inhibitor from Epimedium wanshanense, increases cellular cGMP by enhancing NOS in diabetic ED rats corpus cavernosum tissue".ANDROLOGIA suppl.1(2012):87-93.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Zhang, J.]的文章
[Wang, Y. -B.]的文章
[Ma, C. -G.]的文章
百度学术
百度学术中相似的文章
[Zhang, J.]的文章
[Wang, Y. -B.]的文章
[Ma, C. -G.]的文章
必应学术
必应学术中相似的文章
[Zhang, J.]的文章
[Wang, Y. -B.]的文章
[Ma, C. -G.]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。