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Genotyping cytomegalovirus UL97 mutations by high-resolution melting analysis with unlabeled probe
Zhao, Xiao-Tao1; Zhou, Dan-Qiu2; Wu, Shuai3; Chen, Yue-Wen3; Shao, Yong4,5,6; Zhang, Jie4,5,6; Xia, Chang-Sheng1; Wang, Ke-Peng5; Yang, Hong4,5; Wan, Jun4,5,7; Yu, Bo4,5,6; Zhang, Zheng1; Zhang, Wei3,4,5
刊名ARCHIVES OF VIROLOGY
2012-03-01
DOI10.1007/s00705-011-1173-y
157期:3页:475-481
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Virology
资助者Shenzhen Science and Technology Project ; Shenzhen Science and Technology Project
研究领域[WOS]Virology
关键词[WOS]IMMUNOCOMPROMISED PATIENTS ; GANCICLOVIR RESISTANCE ; TRANSPLANT RECIPIENTS ; PHOSPHOTRANSFERASE MUTATIONS ; CONFERRING RESISTANCE ; DRUG-RESISTANCE ; ANTIVIRAL DRUGS ; RAPID DETECTION ; INFECTION ; DISEASE
英文摘要

Human cytomegalovirus (CMV) is an opportunistic pathogen, and infections with this virus can be treated with ganciclovir (GCV). Most GCV-resistant clinical CMV isolates contain a mutation in the UL97 gene. Genotypic assays for diagnostic screening of GCV-resistant CMV have been developed. High-resolution melting analysis (HRMA) with unlabeled probe is considered a perfect tool for this purpose. In this study, we have developed an HRMA-based genotypic test for the detection of UL97 mutations. Wild type and M460V/I mutants of UL97 were constructed. HRMA with unlabeled probe was used as a genotyping method for the detection of M460V/I mutations. The melting peaks obtained directly from PCR products did not enable us to distinguish the wild type from M460 mutants. The sensitivity and accuracy of HRMA were dramatically improved by using unlabeled probe. HRMA with unlabeled probe successfully distinguished M460V from M460I and served well for the detection of M460V/I mutations in clinical samples. HRMA with unlabeled probe proves to be a sensitive and cost-effective genotyping method for the detection of M460 mutations.

语种英语
资助者Shenzhen Science and Technology Project ; Shenzhen Science and Technology Project
WOS记录号WOS:000303471700008
引用统计
被引频次:3[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/67075
专题北京大学第二临床医学院
作者单位1.Ji Nan Univ, Coll Pharm, Guangzhou, Guangdong, Peoples R China
2.Peking Univ, Peoples Hosp, Dept Clin Lab, Beijing, Peoples R China
3.Fudan Univ, Dept Lab Med, Jinshan Hosp, Shanghai 200433, Peoples R China
4.Shenzhen PKU HKUST Med Ctr, Shenzhen Key Lab Translat Med Dermatol, Shenzhen, Guangdong, Peoples R China
5.Shenzhen PKU HKUST Med Ctr, Biomed Res Inst, Shenzhen 518036, Guangdong, Peoples R China
6.Peking Univ, Dept Dermatol, Shenzhen Hosp, Shenzhen, Guangdong, Peoples R China
7.Hong Kong Univ Sci & Technol, Div Life Sci, Hong Kong, Hong Kong, Peoples R China
推荐引用方式
GB/T 7714
Zhao, Xiao-Tao,Zhou, Dan-Qiu,Wu, Shuai,et al. Genotyping cytomegalovirus UL97 mutations by high-resolution melting analysis with unlabeled probe[J]. ARCHIVES OF VIROLOGY,2012,157(3):475-481.
APA Zhao, Xiao-Tao.,Zhou, Dan-Qiu.,Wu, Shuai.,Chen, Yue-Wen.,Shao, Yong.,...&Zhang, Wei.(2012).Genotyping cytomegalovirus UL97 mutations by high-resolution melting analysis with unlabeled probe.ARCHIVES OF VIROLOGY,157(3),475-481.
MLA Zhao, Xiao-Tao,et al."Genotyping cytomegalovirus UL97 mutations by high-resolution melting analysis with unlabeled probe".ARCHIVES OF VIROLOGY 157.3(2012):475-481.
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