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学科主题: 临床医学
题名:
Genotyping cytomegalovirus UL97 mutations by high-resolution melting analysis with unlabeled probe
作者: Zhao, Xiao-Tao1; Zhou, Dan-Qiu2; Wu, Shuai3; Chen, Yue-Wen3; Shao, Yong4,5,6; Zhang, Jie4,5,6; Xia, Chang-Sheng1; Wang, Ke-Peng5; Yang, Hong4,5; Wan, Jun4,5,7; Yu, Bo4,5,6; Zhang, Zheng1; Zhang, Wei3,4,5
刊名: ARCHIVES OF VIROLOGY
发表日期: 2012-03-01
DOI: 10.1007/s00705-011-1173-y
卷: 157, 期:3, 页:475-481
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Virology
研究领域[WOS]: Virology
关键词[WOS]: IMMUNOCOMPROMISED PATIENTS ; GANCICLOVIR RESISTANCE ; TRANSPLANT RECIPIENTS ; PHOSPHOTRANSFERASE MUTATIONS ; CONFERRING RESISTANCE ; DRUG-RESISTANCE ; ANTIVIRAL DRUGS ; RAPID DETECTION ; INFECTION ; DISEASE
英文摘要:

Human cytomegalovirus (CMV) is an opportunistic pathogen, and infections with this virus can be treated with ganciclovir (GCV). Most GCV-resistant clinical CMV isolates contain a mutation in the UL97 gene. Genotypic assays for diagnostic screening of GCV-resistant CMV have been developed. High-resolution melting analysis (HRMA) with unlabeled probe is considered a perfect tool for this purpose. In this study, we have developed an HRMA-based genotypic test for the detection of UL97 mutations. Wild type and M460V/I mutants of UL97 were constructed. HRMA with unlabeled probe was used as a genotyping method for the detection of M460V/I mutations. The melting peaks obtained directly from PCR products did not enable us to distinguish the wild type from M460 mutants. The sensitivity and accuracy of HRMA were dramatically improved by using unlabeled probe. HRMA with unlabeled probe successfully distinguished M460V from M460I and served well for the detection of M460V/I mutations in clinical samples. HRMA with unlabeled probe proves to be a sensitive and cost-effective genotyping method for the detection of M460 mutations.

语种: 英语
项目资助者: Shenzhen Science and Technology Project
WOS记录号: WOS:000303471700008
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/67075
Appears in Collections:北京大学第二临床医学院_期刊论文

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作者单位: 1.Ji Nan Univ, Coll Pharm, Guangzhou, Guangdong, Peoples R China
2.Peking Univ, Peoples Hosp, Dept Clin Lab, Beijing, Peoples R China
3.Fudan Univ, Dept Lab Med, Jinshan Hosp, Shanghai 200433, Peoples R China
4.Shenzhen PKU HKUST Med Ctr, Shenzhen Key Lab Translat Med Dermatol, Shenzhen, Guangdong, Peoples R China
5.Shenzhen PKU HKUST Med Ctr, Biomed Res Inst, Shenzhen 518036, Guangdong, Peoples R China
6.Peking Univ, Dept Dermatol, Shenzhen Hosp, Shenzhen, Guangdong, Peoples R China
7.Hong Kong Univ Sci & Technol, Div Life Sci, Hong Kong, Hong Kong, Peoples R China

Recommended Citation:
Zhao, Xiao-Tao,Zhou, Dan-Qiu,Wu, Shuai,et al. Genotyping cytomegalovirus UL97 mutations by high-resolution melting analysis with unlabeled probe[J]. ARCHIVES OF VIROLOGY,2012,157(3):475-481.
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