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学科主题: 临床医学
题名:
Highly Sensitive Analysis of Norethisterone in Human Serum by LC Coupled with Atmospheric Pressure Photoionization Tandem Mass Spectrometry
作者: Zhang, Chao1,2; Zhai, Suodi1,2; Liang, Wei3; Jiang, Ji3; Lou, Honggang4
关键词: Column liquid chromatography ; Tandem mass spectrometry ; Norethisterone ; Pharmacokinetics
刊名: CHROMATOGRAPHIA
发表日期: 2009-04-01
DOI: 10.1365/s10337-008-0946-2
卷: 69, 期:7-8, 页:665-670
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]: LIQUID-CHROMATOGRAPHY ; HUMAN PLASMA ; ACETATE ; BIOAVAILABILITY ; ESTRADIOL
英文摘要:

High-performance liquid chromatography coupled with tandem mass spectrometry has been used for sensitive and specific quantitative analysis of norethisterone (NE) in human serum. NE and the internal standard fentanil were isolated by solid-phase extraction. Chromatographic separation was achieved on a 4.6 mm x 50 mm, 5-mu m particle, C(18) column. The mobile phase was 70:30 (v/v) methanol-0.5% aqueous formic acid. NE and the internal standard were detected by multiple-reaction monitoring of precursor/product ion combinations at m/z 299.4/231.2 and 377.1/188.1, respectively; an atmospheric-pressure-photoionization source was used in positive-ion mode. Linearity was established in the concentration range 0.2-49.24 ng mL(-1) and the lowest limit of quantification was 0.2 ng mL(-1). Recovery of NE ranged from 92.54 to 102.74% and relative standard deviations were < 15%. The method was used for a pharmacokinetic study of NE in healthy postmenopausal Chinese female volunteers.

语种: 英语
WOS记录号: WOS:000264885400011
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/67078
Appears in Collections:北京大学第三临床医学院_药剂科_期刊论文

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作者单位: 1.Peking Univ, Hosp 3, Dept Pharm, Beijing 100191, Peoples R China
2.Peking Univ, Therapeut Drug Monitoring & Clin Toxicol Ctr, Beijing 100191, Peoples R China
3.Beijing Union Med Coll Hosp, Dept Pharm, Beijing 100730, Peoples R China
4.Zhejiang Med Univ, Hosp 2, Dept Pharm, Beijing, Peoples R China

Recommended Citation:
Zhang, Chao,Zhai, Suodi,Liang, Wei,et al. Highly Sensitive Analysis of Norethisterone in Human Serum by LC Coupled with Atmospheric Pressure Photoionization Tandem Mass Spectrometry[J]. CHROMATOGRAPHIA,2009,69(7-8):665-670.
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