IR@PKUHSC  > 北京大学第三临床医学院  > 药剂科
学科主题临床医学
Highly Sensitive Analysis of Norethisterone in Human Serum by LC Coupled with Atmospheric Pressure Photoionization Tandem Mass Spectrometry
Zhang, Chao1,2; Zhai, Suodi1,2; Liang, Wei3; Jiang, Ji3; Lou, Honggang4
关键词Column liquid chromatography Tandem mass spectrometry Norethisterone Pharmacokinetics
刊名CHROMATOGRAPHIA
2009-04-01
DOI10.1365/s10337-008-0946-2
69期:7-8页:665-670
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]LIQUID-CHROMATOGRAPHY ; HUMAN PLASMA ; ACETATE ; BIOAVAILABILITY ; ESTRADIOL
英文摘要

High-performance liquid chromatography coupled with tandem mass spectrometry has been used for sensitive and specific quantitative analysis of norethisterone (NE) in human serum. NE and the internal standard fentanil were isolated by solid-phase extraction. Chromatographic separation was achieved on a 4.6 mm x 50 mm, 5-mu m particle, C(18) column. The mobile phase was 70:30 (v/v) methanol-0.5% aqueous formic acid. NE and the internal standard were detected by multiple-reaction monitoring of precursor/product ion combinations at m/z 299.4/231.2 and 377.1/188.1, respectively; an atmospheric-pressure-photoionization source was used in positive-ion mode. Linearity was established in the concentration range 0.2-49.24 ng mL(-1) and the lowest limit of quantification was 0.2 ng mL(-1). Recovery of NE ranged from 92.54 to 102.74% and relative standard deviations were < 15%. The method was used for a pharmacokinetic study of NE in healthy postmenopausal Chinese female volunteers.

语种英语
WOS记录号WOS:000264885400011
引用统计
被引频次:1[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/67078
专题北京大学第三临床医学院_药剂科
北京大学第二临床医学院_产科
作者单位1.Peking Univ, Hosp 3, Dept Pharm, Beijing 100191, Peoples R China
2.Peking Univ, Therapeut Drug Monitoring & Clin Toxicol Ctr, Beijing 100191, Peoples R China
3.Beijing Union Med Coll Hosp, Dept Pharm, Beijing 100730, Peoples R China
4.Zhejiang Med Univ, Hosp 2, Dept Pharm, Beijing, Peoples R China
推荐引用方式
GB/T 7714
Zhang, Chao,Zhai, Suodi,Liang, Wei,et al. Highly Sensitive Analysis of Norethisterone in Human Serum by LC Coupled with Atmospheric Pressure Photoionization Tandem Mass Spectrometry[J]. CHROMATOGRAPHIA,2009,69(7-8):665-670.
APA Zhang, Chao,Zhai, Suodi,Liang, Wei,Jiang, Ji,&Lou, Honggang.(2009).Highly Sensitive Analysis of Norethisterone in Human Serum by LC Coupled with Atmospheric Pressure Photoionization Tandem Mass Spectrometry.CHROMATOGRAPHIA,69(7-8),665-670.
MLA Zhang, Chao,et al."Highly Sensitive Analysis of Norethisterone in Human Serum by LC Coupled with Atmospheric Pressure Photoionization Tandem Mass Spectrometry".CHROMATOGRAPHIA 69.7-8(2009):665-670.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Zhang, Chao]的文章
[Zhai, Suodi]的文章
[Liang, Wei]的文章
百度学术
百度学术中相似的文章
[Zhang, Chao]的文章
[Zhai, Suodi]的文章
[Liang, Wei]的文章
必应学术
必应学术中相似的文章
[Zhang, Chao]的文章
[Zhai, Suodi]的文章
[Liang, Wei]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。