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学科主题: 临床医学
题名:
Identification of two naturally presented MAGE antigenic peptides from a patient with hepatocellular carcinoma by mass spectrometry
作者: Zhou, M; Peng, JR; Zhang, HG; Wang, HX; Zhong, ZH; Pan, XY; Chen, WF; Leng, XS
关键词: cancer/testis antigens ; MHC ligand ; epitope ; acid elution ; HPLC ; mass spectrometry
刊名: IMMUNOLOGY LETTERS
发表日期: 2005-06-15
DOI: 10.1016/j.imlet.2005.02.007
卷: 99, 期:1, 页:113-121
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Immunology
研究领域[WOS]: Immunology
关键词[WOS]: CYTOLYTIC T-LYMPHOCYTES ; CANCER-TESTIS ANTIGENS ; HUMAN GENE MAGE-3 ; LUNG-CANCER ; HEPATITIS-B ; TUMOR ; IMMUNOTHERAPY ; CELLS ; MELANOMA ; EXPRESSION
英文摘要:

In the absence of efficient systemic chemotherapy, immunotherapy is considered a hopeful treatment for controlling recurrence of hepatocellular carcinoma (HCC). The identification of proper antigenic peptides presented by MHC class I molecules is a critical step for the development of therapeutic vaccines against tumors. Currently, the "reverse immunology" approach is the most commonly used technique in the identification of the tumor-associated T cell epitopes. However, it is based on T cell dependent approach and cannot fully reflect the actual presentation of epitope in tumor in vivo. In the present study, we managed to identify the naturally presented MAGE epitopes of HCC directly by epitope prediction, HPLC differential analysis and MS detection. We successfully detected a naturally processed peptide FLWGPRALV (MAGE-3(271-279), HLA-A2-restricted) with an estimated number of 38-39 copies/cell in HCC. To our knowledge, this is the first evidence that the naturally processed MAGE-3(271-279) can be isolated and identified from the tumor tissue of HCC patient. Furthermore, specific CD8+ T cell responses to this epitope were also found after tumor relapse by IFN-gamma release Cytospot and tetramer assay indicating that MAGE-3(271-279) was indeed presented by HCC cells in vivo. In addition, another new antigen peptide was found, which may be derived from MAGE-1. Our findings demonstrate the potential of the direct approach for identification of tumor-associated epitopes. This approach may become a useful tool for the development of vaccine against cancer in the future. (c) 2005 Elsevier B.V. All rights reserved.

语种: 英语
WOS记录号: WOS:000229659300018
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/67081
Appears in Collections:北京大学第二临床医学院_肝胆外科_期刊论文

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作者单位: 1.Peking Univ, Dept Hepatobiliary Surg, People Hosp, Beijing 100044, Peoples R China
2.Peking Univ, Ctr Hlth Sci, Dept Immunol, Sch Basic Med Sci, Beijing 100083, Peoples R China
3.Natl Ctr Biomed Anal, Beijing 100850, Peoples R China
4.Peking Univ, Cent Lab, People Hosp, Beijing 100044, Peoples R China

Recommended Citation:
Zhou, M,Peng, JR,Zhang, HG,et al. Identification of two naturally presented MAGE antigenic peptides from a patient with hepatocellular carcinoma by mass spectrometry[J]. IMMUNOLOGY LETTERS,2005,99(1):113-121.
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