IR@PKUHSC  > 北京大学临床肿瘤学院  > 分子肿瘤学研究室
学科主题临床医学
Identification and elimination of heterophilic antibody interference during antibody pair screening
Wang, Yuan2; Wei, Hanfu3; Pan, Qin3; Wang, Zhaohui2; Xing, Rui1; Li, Wenmei1; Zhang, Jun2; Ding, Meng2; Guo, Jiao2; Wu, Lin2; Lu, Youyong1; Liu, Siqi2
关键词Antibody pair screening High background Enzyme-linked immunosorbent assay Heterophilic antibodies Antigen-mediated affinity chromatography
刊名ANALYTICAL BIOCHEMISTRY
2012-11-01
DOI10.1016/j.ab.2012.07.019
430期:1页:1-3
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemical Research Methods ; Biochemistry & Molecular Biology ; Chemistry, Analytical
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]PROTEIN ; MICROARRAYS ; ASSAY
英文摘要

High background interference during the antibody pair screening process is inevitable. In this study, we found that the high background was associated with heterophilic antibody interference introduced by the application of ascites-derived monoclonal antibodies when conducting large-scale antibody pair screening against different proteins. To eliminate antibody-associated heterophilic antibody interference, both blocking with mouse normal sera and antigen-mediated affinity chromatography were used, resulting in significant improvement in pairing performance and in antibody pair screening efficiency. (c) 2012 Elsevier Inc. All rights reserved.

语种英语
WOS记录号WOS:000309646800001
引用统计
被引频次:1[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/67089
专题北京大学临床肿瘤学院_分子肿瘤学研究室
北京大学口腔医学院_第一门诊部
作者单位1.Beijing Prot Innovat, Beijing 101300, Peoples R China
2.Peking Univ, Canc Hosp Inst, Mol Oncol Lab, Key Lab Carcinogenesis & Translat Res,Minist Educ, Beijing 100142, Peoples R China
3.Chinese Acad Sci, Beijing Inst Genom, Beijing 101300, Peoples R China
推荐引用方式
GB/T 7714
Wang, Yuan,Wei, Hanfu,Pan, Qin,et al. Identification and elimination of heterophilic antibody interference during antibody pair screening[J]. ANALYTICAL BIOCHEMISTRY,2012,430(1):1-3.
APA Wang, Yuan.,Wei, Hanfu.,Pan, Qin.,Wang, Zhaohui.,Xing, Rui.,...&Liu, Siqi.(2012).Identification and elimination of heterophilic antibody interference during antibody pair screening.ANALYTICAL BIOCHEMISTRY,430(1),1-3.
MLA Wang, Yuan,et al."Identification and elimination of heterophilic antibody interference during antibody pair screening".ANALYTICAL BIOCHEMISTRY 430.1(2012):1-3.
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