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学科主题: 临床医学
题名:
Different Wnt-5A gene expressions in the renal cell carcinoma GRC-1 cell line during the cell cycle
作者: Zhuang, LY; Guo, HQ; Xin, DQ; Zhang, ZW; Li, HJ; Yuan, XL; Tang, DQ; Ding, T; Liu, LB; Guo, YL
关键词: Wnt ; renal cell carcinoma ; cell cycle
刊名: CHINESE MEDICAL JOURNAL
发表日期: 2000-04-01
卷: 113, 期:4, 页:306-309
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Medicine, General & Internal
研究领域[WOS]: General & Internal Medicine
关键词[WOS]: WNTS
英文摘要:

Objective To investigate the gene expression at transcription level of growth factor Wnt-5A in different phase during the cell cycle.

Methods We synchronized the renal cell carcinoma GRC-1 cell line by double thymidine blocks and high-pressure N2O gae methods and amplified Wnt-5A cDNAs from different phase using Semi-quantitative RT-PCR (reverse transcriptase polymerase chain reaction). The PCR products were electrophoresised on the agrose gel and detected by Gel Doc 1000 computer controlled system integrating the volumes of each band, representing the intensities of all pixels in a defined band.

Results The different mRNA expressions of growth factor Wnt-5A was detected in RCC GRC-1 cell line. in S phase, the highest level of Wnt-5A transcript was observed, and in G1 and M phase, medial and lowest, respectively, The differences between S and M stages were statistically significant (P < 0.05).

Conclusion Growth factor Wnt-5A has the potential effect on tumorigenesis. It contributes to all phases during cell cycle but in S phase especially.

语种: 英语
WOS记录号: WOS:000087103500004
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/67092
Appears in Collections:北京大学第一临床医学院_泌尿外科_期刊论文

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作者单位: 1.Beijing Med Univ, Dept Physiol, Beijing 100083, Peoples R China
2.Beijing Med Univ, Inst Urol, First Hosp, Dept Urol, Beijing 100083, Peoples R China

Recommended Citation:
Zhuang, LY,Guo, HQ,Xin, DQ,et al. Different Wnt-5A gene expressions in the renal cell carcinoma GRC-1 cell line during the cell cycle[J]. CHINESE MEDICAL JOURNAL,2000,113(4):306-309.
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