IR@PKUHSC  > 北京大学药学院  > 化学生物学系
学科主题药学
Effect of La3+ on osteoblastic differentiation of rat bone marrow stromal cells
Liu, HM; Zhang, TL; Xu, SJ; Wang, K
关键词La3+ marrow stromal cells differentiation
刊名CHINESE SCIENCE BULLETIN
2006
DOI10.1007/s11434-005-0905-3
51期:1页:31-37
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Multidisciplinary Sciences
研究领域[WOS]Science & Technology - Other Topics
关键词[WOS]IN-VITRO ; GROWTH ; CALCIFICATION ; EXPRESSION ; CULTURES
英文摘要

In the present work, the effect of La3+ on osteoblastic differentiation of primary rat bone marrow stromal cells (MSCs) as well as the related mechanisms are studied. Differentiation is monitored by detection of alkaline phosphatase (ALP) activity, osteocalcin secretion, the mRNA levels of Type I collagen and osteocalcin, and matrix mineralization. The results show that La3+ inhibits osteoblastic differentiation of MSCs in the early and middle stages of culture, as demonstrated by the decrease of ALP activity, osteocalcin secretion, and down-regulation of the mRNA level of osteocalcin. However, La3+ does not affect the matrix mineralization in advanced MSCs, because it up-regulates the mRNA levels of Type I collagen, and promotes ALP activity and osteocalcin secretion in MSCs in the late stage of culture. In addition, Western blot analysis exhibits that La3+ induces the phosphorylation and activation of mitogen-activated protein kinase (MAPK). Furthermore, MAPK kinase inhibitor PD98059 completely blocks the inhibitory effect of La3+ on ALP activity of MSCs in the middle stage of culture. These results suggest that La3+ affects MSCs osteoblastic differentiation depending on differentiation stages. La3+ inhibits osteoblastic differentiation of MSCs in the early and middle stages by a MAPK-dependent mechanism, but does not affect the matrix mineralization in advanced MSCs.

语种英语
WOS记录号WOS:000235676600005
Citation statistics
Cited Times:4[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/67093
Collection北京大学药学院_化学生物学系
作者单位1.Peking Univ, Sch Pharmaceut Sci, Dept Chem Biol, Beijing 100083, Peoples R China
2.Huazhong Univ Sci & Technol, Dept Chem, Wuhan 430074, Peoples R China
Recommended Citation
GB/T 7714
Liu, HM,Zhang, TL,Xu, SJ,et al. Effect of La3+ on osteoblastic differentiation of rat bone marrow stromal cells[J]. CHINESE SCIENCE BULLETIN,2006,51(1):31-37.
APA Liu, HM,Zhang, TL,Xu, SJ,&Wang, K.(2006).Effect of La3+ on osteoblastic differentiation of rat bone marrow stromal cells.CHINESE SCIENCE BULLETIN,51(1),31-37.
MLA Liu, HM,et al."Effect of La3+ on osteoblastic differentiation of rat bone marrow stromal cells".CHINESE SCIENCE BULLETIN 51.1(2006):31-37.
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