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Activity of alpha 2,6-Sialyltransferase and its Gene Expression in Peripheral B Lymphocytes in Patients with IgA Nephropathy
Ding, J. -X.; Xu, L. -X.; Zhu, L.; Lv, J. -C.; Zhao, M. -H.; Zhang, H.; Wang, H. -Y.
刊名SCANDINAVIAN JOURNAL OF IMMUNOLOGY
2009-02-01
DOI10.1111/j.1365-3083.2008.02204.x
69期:2页:174-180
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Immunology
研究领域[WOS]Immunology
关键词[WOS]GALACTOSE-DEFICIENT IGA1 ; ABERRANT O-GLYCOSYLATION ; MASS-SPECTROMETRY ; SERUM IGA1 ; GALACTOSYLTRANSFERASE ACTIVITY ; PATHOLOGICAL PHENOTYPES ; HINGE REGION ; SIALYLATION ; GLYCANS
英文摘要

It is known that aberrant sialylation of IgA1 is involved in the pathogenesis of IgA nephropathy (IgAN). We hypothesize that aberrant sialylation of serum IgA1 may result from changes in the activity of alpha 2,6-sialyltransferase (alpha 2,6-ST) or expression of its coding gene ST6GALNAC2 in peripheral B lymphocytes. Sixty patients with IgAN and 20 healthy controls were enrolled. Peripheral B lymphocytes were isolated by CD-19-positive magnetic beads. The expression level of ST6GALNAC2 was quantitatively analysed by real-time reverse-transcriptase polymerase chain reaction (PCR). Serum IgA1 and sialylation levels were detected by enzyme-linked immunosorbent assay (ELISA) and specific lectin-binding ELISA. Activity of alpha 2,6-ST was measured by specific lectin-binding ELISA. Expression of ST6GALNAC2 in B peripheral lymphocytes was significantly lower in patients with IgAN than that in normal controls (3.7 +/- 2.2 versus 6.3 +/- 2.3, P = 0.016); alpha 2,6-ST activity in B lymphocytes was correlated positively with the level of alpha 2,6-sialic acid in serum IgA1 in patients (n = 42) and controls (n = 12) (r = 0.37, P = 0.007). However, alpha 2,6-ST activity did not differ between patients with IgAN and controls (1.19 +/- 1.43 versus 1.06 +/- 1.17, P > 0.05). These data suggested that reduced sialylation of serum IgA1 may result from decreased expression of ST6GALNAC2. The factors affecting activity of alpha 2,6-ST in the sialylation of IgA1 need to be further investigated.

语种英语
WOS记录号WOS:000262694400012
项目编号9852007113 ; 30670981 ; 30670981 ; 30825021 ; 200802052
资助机构&prime ; 985&prime ; program of Peking University ; Foundation of Ministry of Education, China ; national nature science foundation of China ; NSFC Project ; Ministry of Health, China
引用统计
被引频次:12[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/67096
专题北京大学第一临床医学院_肾脏内科
作者单位Peking Univ, Inst Nephrol, Hosp 1, Div Renal, Beijing 100034, Peoples R China
推荐引用方式
GB/T 7714
Ding, J. -X.,Xu, L. -X.,Zhu, L.,et al. Activity of alpha 2,6-Sialyltransferase and its Gene Expression in Peripheral B Lymphocytes in Patients with IgA Nephropathy[J]. SCANDINAVIAN JOURNAL OF IMMUNOLOGY,2009,69(2):174-180.
APA Ding, J. -X..,Xu, L. -X..,Zhu, L..,Lv, J. -C..,Zhao, M. -H..,...&Wang, H. -Y..(2009).Activity of alpha 2,6-Sialyltransferase and its Gene Expression in Peripheral B Lymphocytes in Patients with IgA Nephropathy.SCANDINAVIAN JOURNAL OF IMMUNOLOGY,69(2),174-180.
MLA Ding, J. -X.,et al."Activity of alpha 2,6-Sialyltransferase and its Gene Expression in Peripheral B Lymphocytes in Patients with IgA Nephropathy".SCANDINAVIAN JOURNAL OF IMMUNOLOGY 69.2(2009):174-180.
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