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IR@PKUHSC  > 北京大学第一临床医学院  > 肾脏内科  > 期刊论文
学科主题: 临床医学
题名:
Activity of alpha 2,6-Sialyltransferase and its Gene Expression in Peripheral B Lymphocytes in Patients with IgA Nephropathy
作者: Ding, J. -X.; Xu, L. -X.; Zhu, L.; Lv, J. -C.; Zhao, M. -H.; Zhang, H.; Wang, H. -Y.
刊名: SCANDINAVIAN JOURNAL OF IMMUNOLOGY
发表日期: 2009-02-01
DOI: 10.1111/j.1365-3083.2008.02204.x
卷: 69, 期:2, 页:174-180
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Immunology
研究领域[WOS]: Immunology
关键词[WOS]: GALACTOSE-DEFICIENT IGA1 ; ABERRANT O-GLYCOSYLATION ; MASS-SPECTROMETRY ; SERUM IGA1 ; GALACTOSYLTRANSFERASE ACTIVITY ; PATHOLOGICAL PHENOTYPES ; HINGE REGION ; SIALYLATION ; GLYCANS
英文摘要:

It is known that aberrant sialylation of IgA1 is involved in the pathogenesis of IgA nephropathy (IgAN). We hypothesize that aberrant sialylation of serum IgA1 may result from changes in the activity of alpha 2,6-sialyltransferase (alpha 2,6-ST) or expression of its coding gene ST6GALNAC2 in peripheral B lymphocytes. Sixty patients with IgAN and 20 healthy controls were enrolled. Peripheral B lymphocytes were isolated by CD-19-positive magnetic beads. The expression level of ST6GALNAC2 was quantitatively analysed by real-time reverse-transcriptase polymerase chain reaction (PCR). Serum IgA1 and sialylation levels were detected by enzyme-linked immunosorbent assay (ELISA) and specific lectin-binding ELISA. Activity of alpha 2,6-ST was measured by specific lectin-binding ELISA. Expression of ST6GALNAC2 in B peripheral lymphocytes was significantly lower in patients with IgAN than that in normal controls (3.7 +/- 2.2 versus 6.3 +/- 2.3, P = 0.016); alpha 2,6-ST activity in B lymphocytes was correlated positively with the level of alpha 2,6-sialic acid in serum IgA1 in patients (n = 42) and controls (n = 12) (r = 0.37, P = 0.007). However, alpha 2,6-ST activity did not differ between patients with IgAN and controls (1.19 +/- 1.43 versus 1.06 +/- 1.17, P > 0.05). These data suggested that reduced sialylation of serum IgA1 may result from decreased expression of ST6GALNAC2. The factors affecting activity of alpha 2,6-ST in the sialylation of IgA1 need to be further investigated.

语种: 英语
所属项目编号: 9852007113 ; 30670981 ; 30670981 ; 30825021 ; 200802052
项目资助者: &prime ; 985&prime ; program of Peking University ; Foundation of Ministry of Education, China ; national nature science foundation of China ; NSFC Project ; Ministry of Health, China
WOS记录号: WOS:000262694400012
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/67096
Appears in Collections:北京大学第一临床医学院_肾脏内科_期刊论文

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作者单位: Peking Univ, Inst Nephrol, Hosp 1, Div Renal, Beijing 100034, Peoples R China

Recommended Citation:
Ding, J. -X.,Xu, L. -X.,Zhu, L.,et al. Activity of alpha 2,6-Sialyltransferase and its Gene Expression in Peripheral B Lymphocytes in Patients with IgA Nephropathy[J]. SCANDINAVIAN JOURNAL OF IMMUNOLOGY,2009,69(2):174-180.
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