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学科主题: 药学
题名:
Cytoskeleton reorganization and FAK phosphorylation are involved in lanthanum (III)-promoted proliferation and differentiation in rat osteoblasts
作者: Wang, Xi; Huang, Jian; Zhang, Tianlan; Wang, Kui
关键词: La(3+) ; Osteoblasts ; Proliferation ; Differentiation ; Cytoskeleton ; FAK phosphorylation
刊名: PROGRESS IN NATURAL SCIENCE
发表日期: 2009-03-10
DOI: 10.1016/j.pnsc.2008.04.018
卷: 19, 期:3, 页:331-335
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Materials Science, Multidisciplinary ; Multidisciplinary Sciences
研究领域[WOS]: Materials Science ; Science & Technology - Other Topics
关键词[WOS]: FOCAL ADHESION KINASE ; BINDING-SITES ; CALCIUM-ION ; INTEGRIN ; TRANSDUCTION ; SYSTEMS ; CELLS
英文摘要:

The effect of lanthanum ion (La(3+)) on osteoblast function and cytoskeleton is assessed in vitro. Osteoblasts were isolated from Sprague Dawley fetal neonatal rats. Cell proliferation and gene expression levels of cbfa-1, alkaline phosphatase (ALP), osteocalcin (OC), bone sialoprotein (BSP) and osteopontin (OPN) were examined by cell counting and RT-PCR. Cytoskeleton F-actin was stained with rhodamine-conjugated phalloidin and was visualized by a confocal microscope. As the results, 10(-8)-10(-4) M La(3+)- induced osteoblast proliferation on day 2. Data from the RT-PCR assay revealed that 10(-6) M La(3+) up-regulated the expression levels of ALP, BSP, and cbfa-1 on day 4, while it enhanced the expressions of OC and OP N on day 21. The F-actin cytoskeletonwas strengthened and reorganized under the exposure of La(3+). In addition, the phosphorylation of focal adhesion kinase (FAK) was significantly promoted in 24 h evaluated by Western blot analysis. These findings indicate that La(3+) promotes osteoblast activity through the phosphorylation of FAK and reorganization of the cytoskeleton. (C) 2008 National Natural Science Foundation of China and Chinese Academy of Sciences. Published by Elsevier Limited and Science in China Press. All rights reserved.

语种: 英语
所属项目编号: 20271005 ; 20571006 ; 20637010 ; 2062007
项目资助者: National Natural Science Foundation of China ; Natural Science Foundation of Beijing ; Beijing Municipal Education Commission ; Beijing Talent Project
WOS记录号: WOS:000263557200006
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/67102
Appears in Collections:北京大学药学院_化学生物学系_期刊论文

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作者单位: Peking Univ, Dept Biol Chem, Sch Pharmaceut Sci, Beijing 100083, Peoples R China

Recommended Citation:
Wang, Xi,Huang, Jian,Zhang, Tianlan,et al. Cytoskeleton reorganization and FAK phosphorylation are involved in lanthanum (III)-promoted proliferation and differentiation in rat osteoblasts[J]. PROGRESS IN NATURAL SCIENCE,2009,19(3):331-335.
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