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学科主题药学
Cytoskeleton reorganization and FAK phosphorylation are involved in lanthanum (III)-promoted proliferation and differentiation in rat osteoblasts
Wang, Xi; Huang, Jian; Zhang, Tianlan; Wang, Kui
关键词La(3+) Osteoblasts Proliferation Differentiation Cytoskeleton FAK phosphorylation
刊名PROGRESS IN NATURAL SCIENCE
2009-03-10
DOI10.1016/j.pnsc.2008.04.018
19期:3页:331-335
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Materials Science, Multidisciplinary ; Multidisciplinary Sciences
研究领域[WOS]Materials Science ; Science & Technology - Other Topics
关键词[WOS]FOCAL ADHESION KINASE ; BINDING-SITES ; CALCIUM-ION ; INTEGRIN ; TRANSDUCTION ; SYSTEMS ; CELLS
英文摘要

The effect of lanthanum ion (La(3+)) on osteoblast function and cytoskeleton is assessed in vitro. Osteoblasts were isolated from Sprague Dawley fetal neonatal rats. Cell proliferation and gene expression levels of cbfa-1, alkaline phosphatase (ALP), osteocalcin (OC), bone sialoprotein (BSP) and osteopontin (OPN) were examined by cell counting and RT-PCR. Cytoskeleton F-actin was stained with rhodamine-conjugated phalloidin and was visualized by a confocal microscope. As the results, 10(-8)-10(-4) M La(3+)- induced osteoblast proliferation on day 2. Data from the RT-PCR assay revealed that 10(-6) M La(3+) up-regulated the expression levels of ALP, BSP, and cbfa-1 on day 4, while it enhanced the expressions of OC and OP N on day 21. The F-actin cytoskeletonwas strengthened and reorganized under the exposure of La(3+). In addition, the phosphorylation of focal adhesion kinase (FAK) was significantly promoted in 24 h evaluated by Western blot analysis. These findings indicate that La(3+) promotes osteoblast activity through the phosphorylation of FAK and reorganization of the cytoskeleton. (C) 2008 National Natural Science Foundation of China and Chinese Academy of Sciences. Published by Elsevier Limited and Science in China Press. All rights reserved.

语种英语
WOS记录号WOS:000263557200006
项目编号20271005 ; 20571006 ; 20637010 ; 2062007
资助机构National Natural Science Foundation of China ; Natural Science Foundation of Beijing ; Beijing Municipal Education Commission ; Beijing Talent Project
引用统计
被引频次:13[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/67102
专题北京大学药学院_化学生物学系
北京大学第三临床医学院_骨科
作者单位Peking Univ, Dept Biol Chem, Sch Pharmaceut Sci, Beijing 100083, Peoples R China
推荐引用方式
GB/T 7714
Wang, Xi,Huang, Jian,Zhang, Tianlan,et al. Cytoskeleton reorganization and FAK phosphorylation are involved in lanthanum (III)-promoted proliferation and differentiation in rat osteoblasts[J]. PROGRESS IN NATURAL SCIENCE,2009,19(3):331-335.
APA Wang, Xi,Huang, Jian,Zhang, Tianlan,&Wang, Kui.(2009).Cytoskeleton reorganization and FAK phosphorylation are involved in lanthanum (III)-promoted proliferation and differentiation in rat osteoblasts.PROGRESS IN NATURAL SCIENCE,19(3),331-335.
MLA Wang, Xi,et al."Cytoskeleton reorganization and FAK phosphorylation are involved in lanthanum (III)-promoted proliferation and differentiation in rat osteoblasts".PROGRESS IN NATURAL SCIENCE 19.3(2009):331-335.
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