|Expression of methionine adenosyltransferase 2A in renal cell carcinomas and potential mechanism for kidney carcinogenesis|
|Wang, Xuliang1,2; Guo, Xiaoqiang1; Yu, Wenshui2,3; Li, Cailing2; Gui, Yaoting2; Cai, Zhiming1,2|
|关键词||Methionine adenosyltransferase 2A Renal cell carcinomas S-adenosylmethionine Heme oxygenase-1|
|WOS标题词||Science & Technology|
|关键词[WOS]||S-ADENOSYLMETHIONINE ; CANCER CELLS ; MAT2A GENE ; HISTONE METHYLATION ; FUMARATE-HYDRATASE ; DNA METHYLATION ; COLON-CANCER ; METABOLISM ; APOPTOSIS ; MODULATION|
Background: Methionine adenosyltransferase 2A (MAT2A) is an enzyme that catalyzes the formation of S-adenosylmethionine (SAMe) by joining methionine and ATP. SAMe is a methyl donor for transmethylation and has an important role for DNA and/ or protein methylation. MAT2A is expressed widely in many tissues especially in kidney. Several studies have demonstrated that there are abnormal expressions of MAT2A in several kinds of cancers such as liver and colon cancers. But the relationship of MAT2A between renal cell carcinomas (RCC) is less understood.
Methods: The mRNA expression level of the MAT2A gene was determined in 24 RCC patients and 4 RCC cell lines, using real-time quantitative-polymerase chain reaction (RT-PCR). The MAT2A protein content was measured by western blotting and immunohistochemical analysis in 55 RCC patients. The mRNA levels of heme oxygenase-1 (HO-1) and cyclooxygenase-2 (COX-2) were also analysized in patients using RT-PCR. The correlations between the MAT2A and HO-1 as well as COX-2 were analyzed with nonparametric Spearman method.
Results: MAT2A transcript was significantly downregulated in cancer tissues compared to normal tissues (P < 0.05). Immunohistochemical analysis and western blotting indicated that level of MAT2A protein was decreased in cancer tissues. The statistical analysis reveals a negative correlation between MAT2A and HO-1 expression in RCC patients and cell lines (P < 0.01).
Conclusions: This study demonstrated that MAT2A was lower expression in cancer tissues, suggesting that it may be involved in the development of RCC. MAT2A is a transcriptional corepressor for HO-1 expression by supplying SAM for methyltransferases, which may be one of potential mechanism of MAT2A as tumor suppressor in kidney carcinogenesis.
|项目编号||2012001 ; ZDSY20120615154448514 ; 81270740|
|资助机构||Emerging Scientist Project of Shenzhen Second People&prime ; s Hospital ; promotion Program for Shenzhen Key Laboratory ; National Natural Science Foundation of China|
|作者单位||1.Shenzhen Univ, Affiliated Hosp 1, Shenzhen Peoples Hosp 2, Shenzhen Key Lab Genitourinary Tumor, Shenzhen 518035, Guangdong, Peoples R China|
2.Peking Univ, Shenzhen Hosp, Shenzhen PKU HKUST Med Ctr,Dept Urol, Guangdong & Shenzhen Key Lab Male Reprod Med & Ge, Shenzhen 518036, Guangdong, Peoples R China
3.Shantou Univ, Coll Med, Shantou 515041, Guangdong, Peoples R China
|Wang, Xuliang,Guo, Xiaoqiang,Yu, Wenshui,et al. Expression of methionine adenosyltransferase 2A in renal cell carcinomas and potential mechanism for kidney carcinogenesis[J]. BMC CANCER,2014,14.|
|APA||Wang, Xuliang,Guo, Xiaoqiang,Yu, Wenshui,Li, Cailing,Gui, Yaoting,&Cai, Zhiming.(2014).Expression of methionine adenosyltransferase 2A in renal cell carcinomas and potential mechanism for kidney carcinogenesis.BMC CANCER,14.|
|MLA||Wang, Xuliang,et al."Expression of methionine adenosyltransferase 2A in renal cell carcinomas and potential mechanism for kidney carcinogenesis".BMC CANCER 14(2014).|