IR@PKUHSC  > 北京大学深圳医院
学科主题临床医学
Expression of methionine adenosyltransferase 2A in renal cell carcinomas and potential mechanism for kidney carcinogenesis
Wang, Xuliang1,2; Guo, Xiaoqiang1; Yu, Wenshui2,3; Li, Cailing2; Gui, Yaoting2; Cai, Zhiming1,2
关键词Methionine adenosyltransferase 2A Renal cell carcinomas S-adenosylmethionine Heme oxygenase-1
刊名BMC CANCER
2014-03-17
DOI10.1186/1471-2407-14-196
14
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Oncology
研究领域[WOS]Oncology
关键词[WOS]S-ADENOSYLMETHIONINE ; CANCER CELLS ; MAT2A GENE ; HISTONE METHYLATION ; FUMARATE-HYDRATASE ; DNA METHYLATION ; COLON-CANCER ; METABOLISM ; APOPTOSIS ; MODULATION
英文摘要

Background: Methionine adenosyltransferase 2A (MAT2A) is an enzyme that catalyzes the formation of S-adenosylmethionine (SAMe) by joining methionine and ATP. SAMe is a methyl donor for transmethylation and has an important role for DNA and/ or protein methylation. MAT2A is expressed widely in many tissues especially in kidney. Several studies have demonstrated that there are abnormal expressions of MAT2A in several kinds of cancers such as liver and colon cancers. But the relationship of MAT2A between renal cell carcinomas (RCC) is less understood.

Methods: The mRNA expression level of the MAT2A gene was determined in 24 RCC patients and 4 RCC cell lines, using real-time quantitative-polymerase chain reaction (RT-PCR). The MAT2A protein content was measured by western blotting and immunohistochemical analysis in 55 RCC patients. The mRNA levels of heme oxygenase-1 (HO-1) and cyclooxygenase-2 (COX-2) were also analysized in patients using RT-PCR. The correlations between the MAT2A and HO-1 as well as COX-2 were analyzed with nonparametric Spearman method.

Results: MAT2A transcript was significantly downregulated in cancer tissues compared to normal tissues (P < 0.05). Immunohistochemical analysis and western blotting indicated that level of MAT2A protein was decreased in cancer tissues. The statistical analysis reveals a negative correlation between MAT2A and HO-1 expression in RCC patients and cell lines (P < 0.01).

Conclusions: This study demonstrated that MAT2A was lower expression in cancer tissues, suggesting that it may be involved in the development of RCC. MAT2A is a transcriptional corepressor for HO-1 expression by supplying SAM for methyltransferases, which may be one of potential mechanism of MAT2A as tumor suppressor in kidney carcinogenesis.

语种英语
WOS记录号WOS:000334530000001
引用统计
被引频次:5[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/67107
专题北京大学深圳医院
作者单位1.Shenzhen Univ, Affiliated Hosp 1, Shenzhen Peoples Hosp 2, Shenzhen Key Lab Genitourinary Tumor, Shenzhen 518035, Guangdong, Peoples R China
2.Peking Univ, Shenzhen Hosp, Shenzhen PKU HKUST Med Ctr,Dept Urol, Guangdong & Shenzhen Key Lab Male Reprod Med & Ge, Shenzhen 518036, Guangdong, Peoples R China
3.Shantou Univ, Coll Med, Shantou 515041, Guangdong, Peoples R China
推荐引用方式
GB/T 7714
Wang, Xuliang,Guo, Xiaoqiang,Yu, Wenshui,et al. Expression of methionine adenosyltransferase 2A in renal cell carcinomas and potential mechanism for kidney carcinogenesis[J]. BMC CANCER,2014,14.
APA Wang, Xuliang,Guo, Xiaoqiang,Yu, Wenshui,Li, Cailing,Gui, Yaoting,&Cai, Zhiming.(2014).Expression of methionine adenosyltransferase 2A in renal cell carcinomas and potential mechanism for kidney carcinogenesis.BMC CANCER,14.
MLA Wang, Xuliang,et al."Expression of methionine adenosyltransferase 2A in renal cell carcinomas and potential mechanism for kidney carcinogenesis".BMC CANCER 14(2014).
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Wang, Xuliang]的文章
[Guo, Xiaoqiang]的文章
[Yu, Wenshui]的文章
百度学术
百度学术中相似的文章
[Wang, Xuliang]的文章
[Guo, Xiaoqiang]的文章
[Yu, Wenshui]的文章
必应学术
必应学术中相似的文章
[Wang, Xuliang]的文章
[Guo, Xiaoqiang]的文章
[Yu, Wenshui]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。