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学科主题基础医学
In vitro growth inhibition of human colonic tumor cells by Verapamil
Cao, Qi-Zhen1; Niu, Gang2; Tan, Huan-Ran1
关键词HCT Verapamil
刊名WORLD JOURNAL OF GASTROENTEROLOGY
2005-04-21
11期:15页:2255-2259
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Gastroenterology & Hepatology
资助者Foundation of the National New Drug Research of China ; Foundation of the National New Drug Research of China
研究领域[WOS]Gastroenterology & Hepatology
关键词[WOS]CALCIUM-CHANNEL ANTAGONISTS ; FACTOR STIMULATION ; APOPTOSIS ; MECHANISMS ; DEATH ; LINE
英文摘要

AIM: To investigate the effects and mechanisms of Verapamil on cultured human colonic tumor (HCT) cells.

METHODS: HCT cells were treated with different concentrations of Verapamil, and their proliferation was examined by MTT assay. The areas of sub-diploid peak were measured by flow cytometry, and the DNA ladder was found by agarose gel electrophoresis. The characteristic changes in morphology were observed under light microscopy. The cell nuclei (propidium iodide labeled, PI-labeled) and cellular distribution and concentration of calcium (Fluo-3-labeled) were studied by using laser confocal scanning microscope.

RESULTS: The proliferation of HCT cells was inhibited by different concentrations of Verapamil. With the increase in concentration of Verapamil, the percent of G0-G1 phase cells in HCT cells increased and that of S phase cells decreased. After treating with different concentrations of Verapamil, flow cytometry showed that HCT cells were enlarged in areas of sub-diploid in a dose-dependent manner. Gel electrophoresis results displayed a typical DNA ladder. On staining with Wrights-Giemsa, the typical cellular apoptosis morphologic changes were also observed. PI-labeled cell nuclei were found markedly changed. In addition, we inspected that the 100 mu mol/L Verapamil could increase the intracellular calcium ion concentration [Ca2+](i) in HCT cells.

CONCLUSION: Verapamil can inhibit proliferation of HCT cells via inducing cell apoptosis. (C) 2005 The WJG Press and Elsevier Inc. All rights reserved.

语种英语
所属项目编号96-901-05-197
资助者Foundation of the National New Drug Research of China ; Foundation of the National New Drug Research of China
WOS记录号WOS:000208102700009
引用统计
被引频次:6[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/67113
专题基础医学院_药理学系
作者单位1.Beijing N& N Genetech Co, Beijing 100088, Peoples R China
2.Peking Univ, Dept Pharmacol, Peking Univ Hlth Sci Ctr, Beijing 100083, Peoples R China
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Cao, Qi-Zhen,Niu, Gang,Tan, Huan-Ran. In vitro growth inhibition of human colonic tumor cells by Verapamil[J]. WORLD JOURNAL OF GASTROENTEROLOGY,2005,11(15):2255-2259.
APA Cao, Qi-Zhen,Niu, Gang,&Tan, Huan-Ran.(2005).In vitro growth inhibition of human colonic tumor cells by Verapamil.WORLD JOURNAL OF GASTROENTEROLOGY,11(15),2255-2259.
MLA Cao, Qi-Zhen,et al."In vitro growth inhibition of human colonic tumor cells by Verapamil".WORLD JOURNAL OF GASTROENTEROLOGY 11.15(2005):2255-2259.
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