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Validation of multiple annealing and looping-based amplification cycle sequencing for 24-chromosome aneuploidy screening of cleavage-stage embryos
Huang, Jin1,3,4; Yan, Liying1,3,4; Fan, Wei2; Zhao, Nan1; Zhang, Yan1,3,4; Tang, Fuchou2; Xie, X. Sunney5; Qiao, Jie1,3,4
关键词Cleavage embryo 24-chromosome aneuploidy screening comparative genomic hybridization (CGH) single nucleotide polymorphism (SNP) multiple annealing and looping-based amplification cycles (MALBAC)
刊名FERTILITY AND STERILITY
2014-12-01
DOI10.1016/j.fertnstert.2014.08.015
102期:6页:1685-1691
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Obstetrics & Gynecology ; Reproductive Biology
研究领域[WOS]Obstetrics & Gynecology ; Reproductive Biology
关键词[WOS]COMPARATIVE GENOMIC HYBRIDIZATION ; PREIMPLANTATION GENETIC DIAGNOSIS ; ADVANCED MATERNAL AGE ; SELF-CORRECTION ; CHROMOSOME-ABNORMALITIES ; SINGLE-NUCLEOTIDE ; ANALYSIS REVEALS ; POLAR BODY ; CELL ; BLASTOMERES
英文摘要

Objective: To validate multiple annealing and looping-based amplification cycle (MALBAC) sequencing for 24-chromosome aneuploidy screening of cleavage embryos and to explore the chromosomal characteristics of embryos at the cleavage stage.

Design: The 24-chromosome aneuploidy analyses of the blastomeres included comparative genomic hybridization (CGH), single nucleotide polymorphism (SNP), and MALBAC sequencing.

Setting: University-affiliated IVF center.

Patient(s): Three couples who delivered babies from the same IVF cycle, which included 23 donated, frozen cleavage embryos.

Intervention(s): None.

Main Outcome Measure(s): Three blastomeres were selected from each single embryo and subject to CGH, SNP, and MALBAC sequencing for 24-chromosome aneuploidy, respectively. The results of MALBAC sequencing were compared with the results of CGH and SNP. The chromosomal status and occurrence of the abnormal chromosomes were investigated. The relationship between the embryos′ morphology and the euploid state was analyzed.

Result(s): Among the 23 donated embryos, the MALBAC sequencing results of 18 (78.26%) embryos were identical to those of CGH or SNP, including 8 embryos that had identical results by all three techniques. In terms of euploidy, only 6 of these 23 embryos (26.09%) were diploid. Blastomere abnormality was observed in all autosomes and sex chromosomes. In addition, the frequency of abnormality was different for certain chromosomes.

Conclusion(s): With sequencing at 0.04 x genome depth, MALBAC sequencing has been validated as a satisfactory method for 24-chromosome aneuploidy screening. The proportion of abnormal chromosomes was high in cleavage-stage embryos, and any chromosome could be abnormal. (C) 2014 by American Society for Reproductive Medicine.

语种英语
WOS记录号WOS:000345613600033
项目编号Z131100005213006 ; 31230047
资助机构Peking-Tsinghua Center for Life Sciences ; Beijing Municipal Science and Technology Commission ; National Natural Science of China
引用统计
被引频次:18[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/67122
专题北京大学第三临床医学院_妇产科
北京大学第三临床医学院_生殖医学中心
作者单位1.Harvard Univ, Dept Chem & Chem Biol, Cambridge, MA 02138 USA
2.Peking Univ, Hosp 3, Reprod Med Ctr, Dept Obstet & Gynecol, Beijing 100871, Peoples R China
3.Peking Univ, Coll Life Sci, Biodynam Opt Imaging Ctr, Beijing 100871, Peoples R China
4.Minist Educ, Key Lab Assisted Reprod, Beijing, Peoples R China
5.Key Lab Reprod Endocrinol & Assisted Reprod Techn, Beijing, Peoples R China
推荐引用方式
GB/T 7714
Huang, Jin,Yan, Liying,Fan, Wei,et al. Validation of multiple annealing and looping-based amplification cycle sequencing for 24-chromosome aneuploidy screening of cleavage-stage embryos[J]. FERTILITY AND STERILITY,2014,102(6):1685-1691.
APA Huang, Jin.,Yan, Liying.,Fan, Wei.,Zhao, Nan.,Zhang, Yan.,...&Qiao, Jie.(2014).Validation of multiple annealing and looping-based amplification cycle sequencing for 24-chromosome aneuploidy screening of cleavage-stage embryos.FERTILITY AND STERILITY,102(6),1685-1691.
MLA Huang, Jin,et al."Validation of multiple annealing and looping-based amplification cycle sequencing for 24-chromosome aneuploidy screening of cleavage-stage embryos".FERTILITY AND STERILITY 102.6(2014):1685-1691.
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