北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 北京大学第三临床医学院  > 生殖医学中心  > 期刊论文
学科主题: 临床医学
题名:
Validation of multiple annealing and looping-based amplification cycle sequencing for 24-chromosome aneuploidy screening of cleavage-stage embryos
作者: Huang, Jin1,3,4; Yan, Liying1,3,4; Fan, Wei2; Zhao, Nan1; Zhang, Yan1,3,4; Tang, Fuchou2; Xie, X. Sunney5; Qiao, Jie1,3,4
关键词: Cleavage embryo ; 24-chromosome aneuploidy screening ; comparative genomic hybridization (CGH) ; single nucleotide polymorphism (SNP) ; multiple annealing and looping-based amplification cycles (MALBAC)
刊名: FERTILITY AND STERILITY
发表日期: 2014-12-01
DOI: 10.1016/j.fertnstert.2014.08.015
卷: 102, 期:6, 页:1685-1691
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Obstetrics & Gynecology ; Reproductive Biology
研究领域[WOS]: Obstetrics & Gynecology ; Reproductive Biology
关键词[WOS]: COMPARATIVE GENOMIC HYBRIDIZATION ; PREIMPLANTATION GENETIC DIAGNOSIS ; ADVANCED MATERNAL AGE ; SELF-CORRECTION ; CHROMOSOME-ABNORMALITIES ; SINGLE-NUCLEOTIDE ; ANALYSIS REVEALS ; POLAR BODY ; CELL ; BLASTOMERES
英文摘要:

Objective: To validate multiple annealing and looping-based amplification cycle (MALBAC) sequencing for 24-chromosome aneuploidy screening of cleavage embryos and to explore the chromosomal characteristics of embryos at the cleavage stage.

Design: The 24-chromosome aneuploidy analyses of the blastomeres included comparative genomic hybridization (CGH), single nucleotide polymorphism (SNP), and MALBAC sequencing.

Setting: University-affiliated IVF center.

Patient(s): Three couples who delivered babies from the same IVF cycle, which included 23 donated, frozen cleavage embryos.

Intervention(s): None.

Main Outcome Measure(s): Three blastomeres were selected from each single embryo and subject to CGH, SNP, and MALBAC sequencing for 24-chromosome aneuploidy, respectively. The results of MALBAC sequencing were compared with the results of CGH and SNP. The chromosomal status and occurrence of the abnormal chromosomes were investigated. The relationship between the embryos′ morphology and the euploid state was analyzed.

Result(s): Among the 23 donated embryos, the MALBAC sequencing results of 18 (78.26%) embryos were identical to those of CGH or SNP, including 8 embryos that had identical results by all three techniques. In terms of euploidy, only 6 of these 23 embryos (26.09%) were diploid. Blastomere abnormality was observed in all autosomes and sex chromosomes. In addition, the frequency of abnormality was different for certain chromosomes.

Conclusion(s): With sequencing at 0.04 x genome depth, MALBAC sequencing has been validated as a satisfactory method for 24-chromosome aneuploidy screening. The proportion of abnormal chromosomes was high in cleavage-stage embryos, and any chromosome could be abnormal. (C) 2014 by American Society for Reproductive Medicine.

语种: 英语
所属项目编号: Z131100005213006 ; 31230047
项目资助者: Peking-Tsinghua Center for Life Sciences ; Beijing Municipal Science and Technology Commission ; National Natural Science of China
WOS记录号: WOS:000345613600033
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/67122
Appears in Collections:北京大学第三临床医学院_生殖医学中心_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Harvard Univ, Dept Chem & Chem Biol, Cambridge, MA 02138 USA
2.Peking Univ, Hosp 3, Reprod Med Ctr, Dept Obstet & Gynecol, Beijing 100871, Peoples R China
3.Peking Univ, Coll Life Sci, Biodynam Opt Imaging Ctr, Beijing 100871, Peoples R China
4.Minist Educ, Key Lab Assisted Reprod, Beijing, Peoples R China
5.Key Lab Reprod Endocrinol & Assisted Reprod Techn, Beijing, Peoples R China

Recommended Citation:
Huang, Jin,Yan, Liying,Fan, Wei,et al. Validation of multiple annealing and looping-based amplification cycle sequencing for 24-chromosome aneuploidy screening of cleavage-stage embryos[J]. FERTILITY AND STERILITY,2014,102(6):1685-1691.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Huang, Jin]'s Articles
[Yan, Liying]'s Articles
[Fan, Wei]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Huang, Jin]‘s Articles
[Yan, Liying]‘s Articles
[Fan, Wei]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace