IR@PKUHSC  > 北京大学第一临床医学院  > 男科中心
学科主题临床医学
Effect of gyromagnetic fields on human prostatic adenocarcinoma cells
Lei, Hongen1; Xu, Yongde1; Guan, Ruili1; Li, Meng2; Hui, Yu3; Gao, Zhezhu1; Yang, Bicheng1; Xin, Zhongcheng1
关键词apoptosis gyromagnetic fields PC-3 cells prostate cancer
刊名ONCOTARGETS AND THERAPY
2015
DOI10.2147/OTT.S95306
8页:3489-3497
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biotechnology & Applied Microbiology ; Oncology
研究领域[WOS]Biotechnology & Applied Microbiology ; Oncology
关键词[WOS]PULSED ELECTROMAGNETIC-FIELDS ; STATIC MAGNETIC-FIELDS ; FOLLOW-UP ; CANCER ; GUIDELINES ; MANAGEMENT ; THERAPY ; OSTEOARTHRITIS ; HYPERPLASIA ; CARCINOMA
英文摘要

Purpose: To investigate the biological effect of gyromagnetic fields (GMFs) on cell proliferation and apoptosis of human prostatic adenocarcinoma cells and explore the underlying mechanisms.

Methods: PC-3 cells were grouped into normal control (NC) and GMF treatment groups. Cell proliferation was analyzed with kit-8 and Ki67 immunofluorescence staining, while cell apoptosis was analyzed with flow cytometry double staining of Annexin V-PE/7-AAD. The Akt and p38 MAPK/Caspase signaling pathways were analyzed by western blotting and immunofluorescence staining, and cell polarization was analyzed with PARD3.

Results: Cell proliferation and activity of the Akt pathway were significantly decreased by the GMF, while cell apoptosis, activity of p38 MAPK, and PARD3-positive cell number were significantly increased in the GMF group compared to the NC group.

Conclusion: GMFs inhibit cell proliferation, induce apoptosis, and regulate tumor cell polarity conditions, potentially through down-regulating Akt, activating the p38 MAPK/Caspase pathway, and promoting PARD3 expression in PC-3 cells.

语种英语
WOS记录号WOS:000366036300001
Citation statistics
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/67131
Collection北京大学第一临床医学院_男科中心
作者单位1.Peking Univ, Hosp 1, Androl Ctr, Beijing 100034, Peoples R China
2.Ningxia Med Univ, Gen Hosp, Dept Urol, Yinchuan, Peoples R China
3.Soochow Univ, Affiliated Hosp 1, Dept Urol, Suzhou, Peoples R China
Recommended Citation
GB/T 7714
Lei, Hongen,Xu, Yongde,Guan, Ruili,et al. Effect of gyromagnetic fields on human prostatic adenocarcinoma cells[J]. ONCOTARGETS AND THERAPY,2015,8:3489-3497.
APA Lei, Hongen.,Xu, Yongde.,Guan, Ruili.,Li, Meng.,Hui, Yu.,...&Xin, Zhongcheng.(2015).Effect of gyromagnetic fields on human prostatic adenocarcinoma cells.ONCOTARGETS AND THERAPY,8,3489-3497.
MLA Lei, Hongen,et al."Effect of gyromagnetic fields on human prostatic adenocarcinoma cells".ONCOTARGETS AND THERAPY 8(2015):3489-3497.
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