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学科主题: 临床医学
题名:
Determination of silodosin in human plasma by liquid chromatography-tandem mass spectrometry
作者: Zhao, Xia2; Liu, Yuwang2; Xu, Junyu2; Zhang, Dan1,3; Zhou, Ying2; Gu, Jingkai1; Cui, Yimin2
关键词: Silodosin ; alpha(1A)-Adrenergic receptor antagonist ; LC-MS/MS
刊名: JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
发表日期: 2009-11-01
DOI: 10.1016/j.jchromb.2009.08.025
卷: 877, 期:29, 页:3724-3728
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]: BENIGN PROSTATIC HYPERPLASIA ; ALPHA(1)-ADRENOCEPTOR SUBTYPES ; KMD-3213 ; ANTAGONIST ; TAMSULOSIN
英文摘要:

A rapid, sensitive and specific liquid chromatography-tandem mass spectrometric (LC-MS/MS) method has been developed and validated for the determination of silodosin in human plasma. Silodosin and internal standard (IS) were extracted from human plasma by liquid-liquid extraction using methyl t-butyl ether and analyzed on an Agilent C(8) column with the mobile phase of acetonitrile-10 mM ammonium acetate (40:60, v/v) adjusted to pH 4.5 with acetic acid. Detection was carried out by MS/MS using TurbolonSpray (TIS) ionization and multiple reaction monitoring (MRM) in the positive-ion mode. The mass transitions monitored were m/z 496.3 -> 261.4 and m/z 440.4 -> 259.3 for silodosin and IS, respectively. The standard Curve was linear in the range of 0.50-50.0 ng/ml with intra- and inter-day precision of 3.2-7.2% and 2.0-7.5%, respectively. The lower limit of quantification (LLOQ) for silodosin was 0.50 ng/ml using 500 mu l plasma sample. This method was successfully applied to the pharmacokinetic study in healthy volunteers. (C) 2009 Elsevier B.V. All rights reserved.

语种: 英语
WOS记录号: WOS:000271168100036
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/67139
Appears in Collections:北京大学第一临床医学院_药剂科_期刊论文

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作者单位: 1.Peking Univ, Hosp 1, Dept Pharm, Beijing 100034, Peoples R China
2.Jilin Univ, Res Ctr Drug Metab, Changchun 130023, Peoples R China
3.Peking Univ, Dept Clin Pharmacol, Aerosp Clin Med Coll, Beijing 100049, Peoples R China

Recommended Citation:
Zhao, Xia,Liu, Yuwang,Xu, Junyu,et al. Determination of silodosin in human plasma by liquid chromatography-tandem mass spectrometry[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2009,877(29):3724-3728.
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