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学科主题: 药学
题名:
Gastrointestinal absorption of recombinant hirudin-2 in rats
作者: Yan, XY; Wang, XT; Zhang, XN; Zhang, Q
刊名: JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS
发表日期: 2004-02-01
DOI: 10.1124/jpet.103.056655
卷: 308, 期:2, 页:774-779
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Pharmacology & Pharmacy
研究领域[WOS]: Pharmacology & Pharmacy
关键词[WOS]: EPIDERMAL-GROWTH-FACTOR ; THROMBIN-SPECIFIC INHIBITOR ; INTESTINAL-ABSORPTION ; PROTEASE INHIBITORS ; DRUG-DELIVERY ; NEONATAL PIGS ; FACTOR-I ; INSULIN ; STABILITY ; MEMBRANE
英文摘要:

To investigate the absorption of recombinant hirudin-2 (rHV2) after oral administration to rats and its possible absorption mechanism, a series of experiments were carried out. The degradation of I-125-rHV2 in the luminal contents and variant mucosal subcellular fractions, as well as the effect of degradation inhibition of some adjuvant was investigated. The bioavailability of rHV2, with or without degradation inhibitor after oral administration to rats was estimated, whereas the in situ loop test and everted sac experiment were also conducted to understand more about the gastrointestinal absorption of rHV2 in rats. It was demonstrated that the rHV2 was not stable in the luminal contents and subfraction of the intestinal mucosa. Some enzyme inhibitor, such as bacitracin or casein, could inhibit the degradation to certain degrees. The intact rHV2 molecules were found in the rat plasma after oral administration, and the bioavailability varies obviously, dependent on the analytical method. Some of the enzyme inhibitor could enhance the rHV2 oral absorption. There is no site difference on rHV2 absorption in different segments of small intestine. The possible transport mechanism of rHV2 across the gastrointestinal tract is concerned with the endocytosis process.

语种: 英语
WOS记录号: WOS:000188467800047
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/67146
Appears in Collections:北京大学药学院_药剂学系_期刊论文

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作者单位: Peking Univ, Hlth Sci Ctr, Sch Pharmaceut Sci, Dept Pharmaceut, Beijing 100083, Peoples R China

Recommended Citation:
Yan, XY,Wang, XT,Zhang, XN,et al. Gastrointestinal absorption of recombinant hirudin-2 in rats[J]. JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS,2004,308(2):774-779.
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