IR@PKUHSC  > 北京大学药学院  > 药剂学系
学科主题药学
Gastrointestinal absorption of recombinant hirudin-2 in rats
Yan, XY; Wang, XT; Zhang, XN; Zhang, Q
刊名JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS
2004-02-01
DOI10.1124/jpet.103.056655
308期:2页:774-779
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Pharmacology & Pharmacy
研究领域[WOS]Pharmacology & Pharmacy
关键词[WOS]EPIDERMAL-GROWTH-FACTOR ; THROMBIN-SPECIFIC INHIBITOR ; INTESTINAL-ABSORPTION ; PROTEASE INHIBITORS ; DRUG-DELIVERY ; NEONATAL PIGS ; FACTOR-I ; INSULIN ; STABILITY ; MEMBRANE
英文摘要

To investigate the absorption of recombinant hirudin-2 (rHV2) after oral administration to rats and its possible absorption mechanism, a series of experiments were carried out. The degradation of I-125-rHV2 in the luminal contents and variant mucosal subcellular fractions, as well as the effect of degradation inhibition of some adjuvant was investigated. The bioavailability of rHV2, with or without degradation inhibitor after oral administration to rats was estimated, whereas the in situ loop test and everted sac experiment were also conducted to understand more about the gastrointestinal absorption of rHV2 in rats. It was demonstrated that the rHV2 was not stable in the luminal contents and subfraction of the intestinal mucosa. Some enzyme inhibitor, such as bacitracin or casein, could inhibit the degradation to certain degrees. The intact rHV2 molecules were found in the rat plasma after oral administration, and the bioavailability varies obviously, dependent on the analytical method. Some of the enzyme inhibitor could enhance the rHV2 oral absorption. There is no site difference on rHV2 absorption in different segments of small intestine. The possible transport mechanism of rHV2 across the gastrointestinal tract is concerned with the endocytosis process.

语种英语
WOS记录号WOS:000188467800047
Citation statistics
Cited Times:9[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/67146
Collection北京大学药学院_药剂学系
作者单位Peking Univ, Hlth Sci Ctr, Sch Pharmaceut Sci, Dept Pharmaceut, Beijing 100083, Peoples R China
Recommended Citation
GB/T 7714
Yan, XY,Wang, XT,Zhang, XN,et al. Gastrointestinal absorption of recombinant hirudin-2 in rats[J]. JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS,2004,308(2):774-779.
APA Yan, XY,Wang, XT,Zhang, XN,&Zhang, Q.(2004).Gastrointestinal absorption of recombinant hirudin-2 in rats.JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS,308(2),774-779.
MLA Yan, XY,et al."Gastrointestinal absorption of recombinant hirudin-2 in rats".JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS 308.2(2004):774-779.
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