北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 基础医学院  > 免疫学系  > 期刊论文
学科主题: 基础医学
题名:
Identification of GPR65, a novel regulator of matrix metalloproteinases using high through-put screening
作者: Xu, Hongbo1; Chen, Xiaohong1; Huang, Junwei1; Deng, Weiwei3; Zhong, Qi1; Yue, Changli4; Wang, Pingzhang2,3; Huang, Zhigang1
关键词: Matrix metalloproteinases ; Gene expression regulation ; High through-put screening assay ; GPR65 ; Head and neck cancer
刊名: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
发表日期: 2013-06-21
DOI: 10.1016/j.bbrc.2013.05.065
卷: 436, 期:1, 页:96-103
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology ; Biophysics
研究领域[WOS]: Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]: PROTEIN-COUPLED RECEPTORS ; SQUAMOUS-CELL CARCINOMA ; TUMOR MICROENVIRONMENT ; CANCER ; EXPRESSION ; TDAG8 ; INVASION ; HEAD
英文摘要:

Matrix metalloproteinases (MMPs) are over-expressed in nearly all cancers. To study novel regulatory factors of MMP expression in head and neck cancer (HNC), we screened a total of 636 candidate genes encoding putative human transmembrane proteins using MMP promoter reporter in a dual luciferase assay system. Three genes GPR65, AXL and TNERSF10B dramatically activated the induction of MMP3 expression. The induction of MMP expression by GPR65 was further confirmed in A549 and/or FaDu cells. GPR65 mediated MMP induction under acidic conditions. The AP-1 binding site in MMP3 promoter was crucial for MMP3 induction. Moreover, the A549 cells infected by recombinant adenovirus of GPR65 showed accelerated cell invasion. In conclusion, we validate that GPR65 is vital regulatory genes upstream of MMP3, and define a novel mechanism of MMP3 regulation by proton-sensing G-protein-coupled receptors. (C) 2013 Elsevier Inc. All rights reserved.

语种: 英语
所属项目编号: 81241084 ; 7121005 ; 5122016
项目资助者: National Natural Science Foundation of China ; Beijing Natural Science Foundation
WOS记录号: WOS:000321682100017
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/67159
Appears in Collections:基础医学院_免疫学系_期刊论文

Files in This Item:
File Name/ File Size Content Type Version Access License
1-s2.0-S0006291X13008619-main.pdf(1812KB)期刊论文出版稿限制开放 联系获取全文

作者单位: 1.Capital Med Univ, Dept Otolaryngol Head & Neck Surg, Beijing Tongren Hosp, Key Lab Otolaryngol Head & Neck Surg, Beijing, Peoples R China
2.Peking Univ, Key Lab Med Immunol, Sch Basic Med Sci,Minist Hlth, Dept Immunol,Hlth Sci Ctr,Ctr Human Dis Genom, Beijing 100871, Peoples R China
3.Chinese Natl Human Genome Ctr CHGB Beijing, Funct Genom Grp, Beijing, Peoples R China
4.Capital Med Univ, Beijing Tongren Hosp, Dept Pathol, Beijing, Peoples R China

Recommended Citation:
Xu, Hongbo,Chen, Xiaohong,Huang, Junwei,et al. Identification of GPR65, a novel regulator of matrix metalloproteinases using high through-put screening[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2013,436(1):96-103.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Xu, Hongbo]'s Articles
[Chen, Xiaohong]'s Articles
[Huang, Junwei]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Xu, Hongbo]‘s Articles
[Chen, Xiaohong]‘s Articles
[Huang, Junwei]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace