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学科主题基础医学
Identification of GPR65, a novel regulator of matrix metalloproteinases using high through-put screening
Xu, Hongbo1; Chen, Xiaohong1; Huang, Junwei1; Deng, Weiwei3; Zhong, Qi1; Yue, Changli4; Wang, Pingzhang2,3; Huang, Zhigang1
关键词Matrix metalloproteinases Gene expression regulation High through-put screening assay GPR65 Head and neck cancer
刊名BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
2013-06-21
DOI10.1016/j.bbrc.2013.05.065
436期:1页:96-103
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Biophysics
研究领域[WOS]Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]PROTEIN-COUPLED RECEPTORS ; SQUAMOUS-CELL CARCINOMA ; TUMOR MICROENVIRONMENT ; CANCER ; EXPRESSION ; TDAG8 ; INVASION ; HEAD
英文摘要

Matrix metalloproteinases (MMPs) are over-expressed in nearly all cancers. To study novel regulatory factors of MMP expression in head and neck cancer (HNC), we screened a total of 636 candidate genes encoding putative human transmembrane proteins using MMP promoter reporter in a dual luciferase assay system. Three genes GPR65, AXL and TNERSF10B dramatically activated the induction of MMP3 expression. The induction of MMP expression by GPR65 was further confirmed in A549 and/or FaDu cells. GPR65 mediated MMP induction under acidic conditions. The AP-1 binding site in MMP3 promoter was crucial for MMP3 induction. Moreover, the A549 cells infected by recombinant adenovirus of GPR65 showed accelerated cell invasion. In conclusion, we validate that GPR65 is vital regulatory genes upstream of MMP3, and define a novel mechanism of MMP3 regulation by proton-sensing G-protein-coupled receptors. (C) 2013 Elsevier Inc. All rights reserved.

语种英语
WOS记录号WOS:000321682100017
项目编号81241084 ; 7121005 ; 5122016
资助机构National Natural Science Foundation of China ; Beijing Natural Science Foundation
引用统计
被引频次:6[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/67159
专题北京大学基础医学院_免疫学系
北京大学基础医学院
作者单位1.Capital Med Univ, Dept Otolaryngol Head & Neck Surg, Beijing Tongren Hosp, Key Lab Otolaryngol Head & Neck Surg, Beijing, Peoples R China
2.Peking Univ, Key Lab Med Immunol, Sch Basic Med Sci,Minist Hlth, Dept Immunol,Hlth Sci Ctr,Ctr Human Dis Genom, Beijing 100871, Peoples R China
3.Chinese Natl Human Genome Ctr CHGB Beijing, Funct Genom Grp, Beijing, Peoples R China
4.Capital Med Univ, Beijing Tongren Hosp, Dept Pathol, Beijing, Peoples R China
推荐引用方式
GB/T 7714
Xu, Hongbo,Chen, Xiaohong,Huang, Junwei,et al. Identification of GPR65, a novel regulator of matrix metalloproteinases using high through-put screening[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2013,436(1):96-103.
APA Xu, Hongbo.,Chen, Xiaohong.,Huang, Junwei.,Deng, Weiwei.,Zhong, Qi.,...&Huang, Zhigang.(2013).Identification of GPR65, a novel regulator of matrix metalloproteinases using high through-put screening.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,436(1),96-103.
MLA Xu, Hongbo,et al."Identification of GPR65, a novel regulator of matrix metalloproteinases using high through-put screening".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 436.1(2013):96-103.
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