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学科主题: 基础医学
题名:
Expression and immunohistochemical localization of aquaporin-1 in male reproductive organs of the mouse
作者: Lu, D. Y.3,4; Li, Y.4; Bi, Z. W.4; Yu, H. M.5; Li, X. J.1,2
刊名: ANATOMIA HISTOLOGIA EMBRYOLOGIA
发表日期: 2008-02-01
DOI: 10.1111/j.1439-0264.2007.00827.x
卷: 37, 期:1, 页:1-8
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Anatomy & Morphology ; Veterinary Sciences
研究领域[WOS]: Anatomy & Morphology ; Veterinary Sciences
关键词[WOS]: DUCTULI EFFERENTES TESTIS ; MESSENGER-RNA EXPRESSION ; CHIP28 WATER CHANNEL ; HUMAN VAS-DEFERENS ; EPITHELIAL-CELLS ; SPERM MOTILITY ; FLUID REABSORPTION ; MEMBRANE-PROTEIN ; PROXIMAL TUBULE ; GENE-EXPRESSION
英文摘要:

Aquaporin-1 (AQP-1), a six-transmembrane domain protein, is found to be responsible for water transport. The purpose of this study was to investigate the expression of AQP-1 mRNA and protein in the testis, epididymis, vas deferens, ventral prostate, and seminal vesicle from mature mice by using reverse transcription polymerase chain reaction (RT-PCR) and Western blotting, and the cellular localization of AQP-1 by immunohistochemistry. RT-PCR revealed that AQP-1 mRNA was expressed in all organs we examined. Western blotting displayed a 29-kDa band and a 35- to 45-kDa band corresponding to non-glycosylated and/or glycosylated AQP-1 in those organs. The immumohistochemical evidence showed that AQP-1 was mainly located on the plasma membrane of epithelial cells of the rete testis, vas deferens, ventral prostate seminal vesicle and the non-ciliated cells of the proximal an distal efferent ducts. However, AQP-1 was absent from spermatogenic cells lining the seminiferous epithelium and from the spermatozoa in the lumen of the distal efferent duct. These findings provide valuable information on the expression of AQP-1 in male reproductive organs and suggest that AQP-1 is involved in water transport to regulate water homeostasis in male reproductive physiology.

语种: 英语
WOS记录号: WOS:000253455200001
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/67196
Appears in Collections:基础医学院_药理学系_期刊论文

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作者单位: 1.Peking Univ, Hlth Sci Ctr, Dept Pharmacol, Sch Basic Med Sci, Beijing 100083, Peoples R China
2.Peking Univ, Hlth Sci Ctr, State Key Lab Nat & Biomimet Drugs, Beijing 100083, Peoples R China
3.Grad Univ Chinese Acad Sci, Beijing 100049, Peoples R China
4.Peking Univ, Hlth Sci Ctr, Sch Basic Med Sci, Dept Anat & Histol & Embryol, Beijing 100083, Peoples R China
5.Natl Res Inst Family Planning, Beijing 100081, Peoples R China

Recommended Citation:
Lu, D. Y.,Li, Y.,Bi, Z. W.,et al. Expression and immunohistochemical localization of aquaporin-1 in male reproductive organs of the mouse[J]. ANATOMIA HISTOLOGIA EMBRYOLOGIA,2008,37(1):1-8.
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