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学科主题: 药学
题名:
Synthesis of photolabile dUTP analogues and their enzymatic incorporation for DNA labeling
作者: Wu JunZhou1,2; Wang Jie1,2; Tang XinJing1,2,3,4
关键词: photolabile nucleotides ; enzymatic incorporation ; nucleotide analogues ; fluorescent labeling
刊名: SCIENCE CHINA-CHEMISTRY
发表日期: 2014-02-01
DOI: 10.1007/s11426-013-5034-2
卷: 57, 期:2, 页:322-328
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Chemistry, Multidisciplinary
研究领域[WOS]: Chemistry
关键词[WOS]: NUCLEOSIDE TRIPHOSPHATES ; NUCLEOTIDES ; OLIGONUCLEOTIDES
英文摘要:

Two new photolabile nucleotide analogues with furan-fused deoxyuridine were synthesized through Sonogashira coupling. Their enzymatic incorporation into DNA was evaluated with two DNA polymerases (Taq and Deep vent exo-) by polymerase chain reaction (PCR). Deep vent exo- recognized both nucleotides as substrates for primer extension, while Taq was much less proficient. Light irradiation of PCR products released the amino and carboxyl moieties of DNA. Further labeling with fluorescein isothiocyanate for a long DNA construct with F-dUnTP incorporation was successfully achieved.

语种: 英语
所属项目编号: 21072015 ; 2012CB720600 ; NCET-10-0203 ; SIMM1106KF-15
项目资助者: National Natural Science Foundation of China ; National Basic Research Program of China (973 Program) ; Program for New Century Excellent Talents in University ; State Key Laboratory of Drug Research
WOS记录号: WOS:000330681700015
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/67200
Appears in Collections:北京大学药学院_期刊论文

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作者单位: 1.Peking Univ, State Key Lab Nat & Biomimet Drugs, Beijing 100191, Peoples R China
2.Peking Univ, Sch Pharmaceut Sci, Beijing 100191, Peoples R China
3.Chinese Acad Sci, State Key Lab Drug Res, Shanghai 201203, Peoples R China
4.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China

Recommended Citation:
Wu JunZhou,Wang Jie,Tang XinJing. Synthesis of photolabile dUTP analogues and their enzymatic incorporation for DNA labeling[J]. SCIENCE CHINA-CHEMISTRY,2014,57(2):322-328.
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