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Synthesis of photolabile dUTP analogues and their enzymatic incorporation for DNA labeling
Wu JunZhou1,2; Wang Jie1,2; Tang XinJing1,2,3,4
关键词photolabile nucleotides enzymatic incorporation nucleotide analogues fluorescent labeling
刊名SCIENCE CHINA-CHEMISTRY
2014-02-01
DOI10.1007/s11426-013-5034-2
57期:2页:322-328
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Chemistry, Multidisciplinary
资助者National Natural Science Foundation of China ; National Basic Research Program of China (973 Program) ; Program for New Century Excellent Talents in University ; State Key Laboratory of Drug Research ; National Natural Science Foundation of China ; National Basic Research Program of China (973 Program) ; Program for New Century Excellent Talents in University ; State Key Laboratory of Drug Research
研究领域[WOS]Chemistry
关键词[WOS]NUCLEOSIDE TRIPHOSPHATES ; NUCLEOTIDES ; OLIGONUCLEOTIDES
英文摘要

Two new photolabile nucleotide analogues with furan-fused deoxyuridine were synthesized through Sonogashira coupling. Their enzymatic incorporation into DNA was evaluated with two DNA polymerases (Taq and Deep vent exo-) by polymerase chain reaction (PCR). Deep vent exo- recognized both nucleotides as substrates for primer extension, while Taq was much less proficient. Light irradiation of PCR products released the amino and carboxyl moieties of DNA. Further labeling with fluorescein isothiocyanate for a long DNA construct with F-dUnTP incorporation was successfully achieved.

语种英语
所属项目编号21072015 ; 2012CB720600 ; NCET-10-0203 ; SIMM1106KF-15
资助者National Natural Science Foundation of China ; National Basic Research Program of China (973 Program) ; Program for New Century Excellent Talents in University ; State Key Laboratory of Drug Research ; National Natural Science Foundation of China ; National Basic Research Program of China (973 Program) ; Program for New Century Excellent Talents in University ; State Key Laboratory of Drug Research
WOS记录号WOS:000330681700015
引用统计
被引频次:3[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/67200
专题北京大学药学院
作者单位1.Peking Univ, State Key Lab Nat & Biomimet Drugs, Beijing 100191, Peoples R China
2.Peking Univ, Sch Pharmaceut Sci, Beijing 100191, Peoples R China
3.Chinese Acad Sci, State Key Lab Drug Res, Shanghai 201203, Peoples R China
4.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China
推荐引用方式
GB/T 7714
Wu JunZhou,Wang Jie,Tang XinJing. Synthesis of photolabile dUTP analogues and their enzymatic incorporation for DNA labeling[J]. SCIENCE CHINA-CHEMISTRY,2014,57(2):322-328.
APA Wu JunZhou,Wang Jie,&Tang XinJing.(2014).Synthesis of photolabile dUTP analogues and their enzymatic incorporation for DNA labeling.SCIENCE CHINA-CHEMISTRY,57(2),322-328.
MLA Wu JunZhou,et al."Synthesis of photolabile dUTP analogues and their enzymatic incorporation for DNA labeling".SCIENCE CHINA-CHEMISTRY 57.2(2014):322-328.
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