|Effects of nitric oxide and hydrogen sulfide on the relaxation of pulmonary arteries in rats|
|Wang Yan-Fei1; Mainali, Prabha1; Tang Chao-Shu2,3; Shi Lin1; Zhang Chun-Yu1; Yan Hui1; Liu Xue-Qin1; Du Jun-Bao1|
|关键词||pulmonary artery nitric oxide hydrogen sulfide relaxation|
|刊名||CHINESE MEDICAL JOURNAL|
|WOS标题词||Science & Technology|
|类目[WOS]||Medicine, General & Internal|
|研究领域[WOS]||General & Internal Medicine|
|关键词[WOS]||VASCULAR SMOOTH-MUSCLE ; RELAXING FACTOR ; L-ARGININE ; HYPERTENSION ; PATHOGENESIS ; PATHWAY ; CELLS ; VEIN ; H2S|
Background The balance between vasodilation and vasoconstriction plays a major role in maintaining vascular homeostasis. However, the underlying mechanisms are unclear. More and more evidence suggested that there was an interaction in the regulation of vasorelaxation between nitric oxide (NO) and hydrogen sulfide (H(2)S). We explored the interaction between and effects of NO and H(2)S on the relaxation of pulmonary arteries in rats.
Methods Seven male Sprague-Dawley rats were anaesthetized with chloral hydrate and the pulmonary arteries of each rat separated for the study of vascular activities. The vasorelaxing activities of pulmonary artery rings in response to different doses of a NO donor, sodium nitroprusside (SNP), or a H(2)S donor, sodium hydrogensulfide (NaHS), were measured in vitro. When pulmonary artery rings were treated with a cystathionine-gamma-lyase inhibitor, DL-propargylglycine, in the presence of SNP or a nitric oxide synthase inhibitor, N(omega)-nitro-L-arginine methyl ester, in the presence of NaHS, the changes in relaxing activities were analyzed.
Results The relaxation of pulmonary artery rings was in a dose dependent manner in response to either SNP or NaHS. The relaxation rates of pulmonary artery rings increased from (30.90 +/- 4.62) % to (60.50 +/- 8.08) % when the concentration of SNP increased from 1 mu mol/L to 3 mu mol/L and from (26.13 +/- 4.12) % to (53.09 +/- 14.01) % when the concentration of NaHS increased from 25 mu mol/L to 100 mu mol/L. However, when appropriate inhibitor was added, the relaxation responses to SNP and NaHS decreased.
Conclusions The results suggested that similarly to NO, H(2)S acted as a vasorelaxant either independently of, or synergistically with NO in the regulation of vasorelaxation. The interaction between NO and H(2)S played an important role in regulating relaxing activities of pulmonary arteries.
|作者单位||1.Peking Univ, Hosp 1, Dept Pediat, Beijing 100034, Peoples R China|
2.Peking Univ, Hosp 1, Inst Cardiovasc Res, Beijing 100034, Peoples R China
3.Minist Educ, Key Lab Mol Cardiovasc Res, Beijing 100034, Peoples R China
|Wang Yan-Fei,Mainali, Prabha,Tang Chao-Shu,et al. Effects of nitric oxide and hydrogen sulfide on the relaxation of pulmonary arteries in rats[J]. CHINESE MEDICAL JOURNAL,2008,121(5):420-423.|
|APA||Wang Yan-Fei.,Mainali, Prabha.,Tang Chao-Shu.,Shi Lin.,Zhang Chun-Yu.,...&Du Jun-Bao.(2008).Effects of nitric oxide and hydrogen sulfide on the relaxation of pulmonary arteries in rats.CHINESE MEDICAL JOURNAL,121(5),420-423.|
|MLA||Wang Yan-Fei,et al."Effects of nitric oxide and hydrogen sulfide on the relaxation of pulmonary arteries in rats".CHINESE MEDICAL JOURNAL 121.5(2008):420-423.|