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Biotransformation of columbianadin by rat hepatic microsomes and inhibition of biotransformation products on NO production in RAW 264.7 cells in vitro
Zhang, You-Bo; Li, Wei; Yang, Xiu-Wei
关键词Angelica pubescens f. biserrata Umbelliferae Biotransformation Columbianadin Columbianadiratimetins A-H Nitric oxide
刊名PHYTOCHEMISTRY
2012-09-01
DOI10.1016/j.phytochem.2012.06.015
81页:109-116
收录类别SCI ; IC
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Plant Sciences
研究领域[WOS]Biochemistry & Molecular Biology ; Plant Sciences
关键词[WOS]MECHANISM-BASED INACTIVATION ; HUMAN LIVER ; UMBELLIFEROUS PLANTS ; CYTOCHROME-P450 2A6 ; PEUCEDANUM-PALUSTRE ; COUMARIN ; METABOLISM ; CARBOXYLESTERASES ; CONSTITUENTS ; MYRISLIGNAN
英文摘要

Columbianadin (CBN, 1), 1-[(8S)-8,9-dihydro-2-oxo-2H-furo[2,3-h]-1-benzopyran-8-yl]-1-methylethyl-[(2Z)-2-methyl-2-butenoic acid jester is a coumarin-type compound and one of the main bioactive constituents of the underground part of Angelica pubescens Maxim. f. biserrata Shan et Yuan. Although numerous investigations have been undertaken to study the biological activities of CBN, such as analgesic, anti-inflammatory, calcium-channel blocking, and platelet aggregation inhibiting functions, little attention has been paid to its metabolism and/or biotransformation. Biotransformation of CBN by rat liver microsomes in vitro was studied, and thirteen biotransformation products including eight hitherto unknown compounds [columbianadiratimetins A-H (3-10)] and five known compounds [columbianadin oxide (2), (+)-2,3-dihydro-4-hydroxy-2-(1-hydroxy-1-methylethyl)-5-benzofurancarboxaldehyde (11), oroselol (12), columbianetin (13), and vaginol (14)] were produced by liver microsomes from rats pre-treated with sodium phenobarbital. The structures of these compounds were elucidated on the basis of extensive spectroscopic analyses which included IR, UV, EIMS, HRESIMS, 1D NMR and 2D NMR, respectively. The inhibition of CBN and its main biotransformation products on nitric oxide production induced by lipopolysaccharide was assayed in RAW 264.7 cells at concentrations ranging from 10 to 200 mu M to evaluate the biological significance of biotransformation. (C) 2012 Elsevier Ltd. All rights reserved.

语种英语
WOS记录号WOS:000308121000012
项目编号2011BAI07B08 ; 30672609 ; 2009ZX09301-010 ; Z0004105040311
资助机构National Key Technology R&amp ; D Program of China ; National Natural Science Foundation of China ; "Major New Medicine Project" in Mega-projects of Science Research of China ; Beijing Municipal Special-purpose Science Foundation of China
引用统计
被引频次:22[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/67206
专题北京大学药学院
作者单位Peking Univ, Hlth Sci Ctr, Sch Pharmaceut Sci, Dept Nat Med,State Key Lab Nat & Biomimet Drugs, Beijing 100191, Peoples R China
推荐引用方式
GB/T 7714
Zhang, You-Bo,Li, Wei,Yang, Xiu-Wei. Biotransformation of columbianadin by rat hepatic microsomes and inhibition of biotransformation products on NO production in RAW 264.7 cells in vitro[J]. PHYTOCHEMISTRY,2012,81:109-116.
APA Zhang, You-Bo,Li, Wei,&Yang, Xiu-Wei.(2012).Biotransformation of columbianadin by rat hepatic microsomes and inhibition of biotransformation products on NO production in RAW 264.7 cells in vitro.PHYTOCHEMISTRY,81,109-116.
MLA Zhang, You-Bo,et al."Biotransformation of columbianadin by rat hepatic microsomes and inhibition of biotransformation products on NO production in RAW 264.7 cells in vitro".PHYTOCHEMISTRY 81(2012):109-116.
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