北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 基础医学院  > 免疫学系  > 期刊论文
学科主题: 基础医学
题名:
Pyronin Y as a fluorescent stain for paraffin sections
作者: Li, B; Wu, Y; Gao, XM
刊名: HISTOCHEMICAL JOURNAL
发表日期: 2002-06-01
卷: 34, 期:6-7, 页:299-303
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Cell Biology
研究领域[WOS]: Cell Biology
关键词[WOS]: NUCLEIC-ACIDS ; HEMATOXYLIN-EOSIN ; CELLS ; RNA ; DNA ; MICROSCOPY ; COMPLEXES ; PHENOTYPE ; DYES
英文摘要:

Pyronin Y has long been used, in combination with other dyes such as Methyl Green, as a differential stain for nucleic acids in paraffin tissue sections. It also forms fluorescent complexes with double-stranded nucleic acids, especially RNA, enabling semi-quantitative analysis of cellular RNA in flow cytometry. However, the possibility of using pyronin Y as a fluorescent stain for paraffin tissue sections has rarely been investigated. We herein report that in sections stained with Methyl Green pyronin Y, red blood cells, elastic fibre of blood vessels, zymogen granules of pancreatic acinar cells, surface membrane of heptocytes and kidney tubular cells showed strikingly strong green and/or red fluorescence, while the nuclei of cells appeared non-fluorescent. The use of confocal laser-scanning microscope greatly improved the resolution and selectivity of the fluorescent images. Staining with pyronin Y alone gave similar results in terms of fluorescence properties of the specimens. Pretreatment of paraffin sections with RNase significantly reduced cytoplasmic pyronin Y staining as judged by transmission light microscopy, but it had little effect on the fluorescence intensity of red blood cells, elastic fibres and zymogen granules.

语种: 英语
WOS记录号: WOS:000182132300005
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/67239
Appears in Collections:基础医学院_免疫学系_期刊论文

Files in This Item:
File Name/ File Size Content Type Version Access License
art%3A10.1023%2FA%3A1023325213198.pdf(364KB)期刊论文出版稿限制开放 联系获取全文

作者单位: Peking Univ, Dept Immunol, Hlth Sci Ctr, Beijing 100083, Peoples R China

Recommended Citation:
Li, B,Wu, Y,Gao, XM. Pyronin Y as a fluorescent stain for paraffin sections[J]. HISTOCHEMICAL JOURNAL,2002,34(6-7):299-303.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Li, B]'s Articles
[Wu, Y]'s Articles
[Gao, XM]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Li, B]‘s Articles
[Wu, Y]‘s Articles
[Gao, XM]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace