IR@PKUHSC  > 北京大学基础医学院  > 免疫学系
学科主题基础医学
Pyronin Y as a fluorescent stain for paraffin sections
Li, B; Wu, Y; Gao, XM
刊名HISTOCHEMICAL JOURNAL
2002-06-01
34期:6-7页:299-303
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Cell Biology
研究领域[WOS]Cell Biology
关键词[WOS]NUCLEIC-ACIDS ; HEMATOXYLIN-EOSIN ; CELLS ; RNA ; DNA ; MICROSCOPY ; COMPLEXES ; PHENOTYPE ; DYES
英文摘要

Pyronin Y has long been used, in combination with other dyes such as Methyl Green, as a differential stain for nucleic acids in paraffin tissue sections. It also forms fluorescent complexes with double-stranded nucleic acids, especially RNA, enabling semi-quantitative analysis of cellular RNA in flow cytometry. However, the possibility of using pyronin Y as a fluorescent stain for paraffin tissue sections has rarely been investigated. We herein report that in sections stained with Methyl Green pyronin Y, red blood cells, elastic fibre of blood vessels, zymogen granules of pancreatic acinar cells, surface membrane of heptocytes and kidney tubular cells showed strikingly strong green and/or red fluorescence, while the nuclei of cells appeared non-fluorescent. The use of confocal laser-scanning microscope greatly improved the resolution and selectivity of the fluorescent images. Staining with pyronin Y alone gave similar results in terms of fluorescence properties of the specimens. Pretreatment of paraffin sections with RNase significantly reduced cytoplasmic pyronin Y staining as judged by transmission light microscopy, but it had little effect on the fluorescence intensity of red blood cells, elastic fibres and zymogen granules.

语种英语
WOS记录号WOS:000182132300005
Citation statistics
Cited Times:5[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/67239
Collection北京大学基础医学院_免疫学系
作者单位Peking Univ, Dept Immunol, Hlth Sci Ctr, Beijing 100083, Peoples R China
Recommended Citation
GB/T 7714
Li, B,Wu, Y,Gao, XM. Pyronin Y as a fluorescent stain for paraffin sections[J]. HISTOCHEMICAL JOURNAL,2002,34(6-7):299-303.
APA Li, B,Wu, Y,&Gao, XM.(2002).Pyronin Y as a fluorescent stain for paraffin sections.HISTOCHEMICAL JOURNAL,34(6-7),299-303.
MLA Li, B,et al."Pyronin Y as a fluorescent stain for paraffin sections".HISTOCHEMICAL JOURNAL 34.6-7(2002):299-303.
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