|Diagnostic value of cancer-testis antigen mRNA in peripheral blood from hepatocellular carcinoma patients|
|Zhao, Li2; Mou, Dong-Cheng2; Peng, Ji-Run1; Huang, Lei1; Wu, Zeng-An2; Leng, Xi-Sheng1|
|关键词||Hepatocellular carcinoma alpha-fetoprotein Cancer-testis antigen Diagnosis Nested reverse transcripts-polymerase chain reaction|
|刊名||WORLD JOURNAL OF GASTROENTEROLOGY|
|WOS标题词||Science & Technology|
|类目[WOS]||Gastroenterology & Hepatology|
|研究领域[WOS]||Gastroenterology & Hepatology|
|关键词[WOS]||POLYMERASE-CHAIN-REACTION ; CIRCULATING TUMOR-CELLS ; ALPHA-FETOPROTEIN ; SOLID TUMORS ; CANCER/TESTIS ANTIGENS ; REVERSE-TRANSCRIPTASE ; REACTION TECHNOLOGY ; BREAST-CANCER ; GENE FAMILY ; MICROMETASTASES|
AIM: To evaluate the diagnostic value of cancer-testis antigen (CTA) mRNA in peripheral blood samples from hepatocellular carcinoma (HCC) patients.
METHODS: Peripheral blood samples were taken from 90 patients with HCC before operation. Expression of melanoma antigen-1 (MAGE-1), synovial sarcoma X break-point-1 (SSX-1), and cancer-testis-associated protein of 11 kDa (CTp11) mRNA in peripheral blood mononuclear cells (PBMC) was tested by nested reverse transcripts-polymerase chain reaction (RT-PCR). Serum a-fetoprotein (AFP) in these patients was also determined.
RESULTS: The positive rate of MAGE-1, SSX-1 and CTp11 transcripts was 37.7%, 34.4%, 31.1% in PBMC samples, and 74.4%, 73.3%, 62.2% in their resected tumor samples, respectively. The positive rate for at least one of the transcripts of three CTA genes was 66.7% in PBMC samples and 91.1% in their resected tumor samples. MAGE-1, SSX-1 and/or CTp11 mRNA were not detected in the PBMC of those patients from whom the resected tumor samples were MAGE-1, SSX-1 and/or CTp11 mRNA negative, nor in the PBMC samples from 20 healthy donors and 10 cirrhotic patients. Among the 90 patients, the serum AFP in 44 patients met the general diagnostic standard (AFP > 400 mu g/L) for HCC, and was negative (AFP <= 20 mu g/L) or positive with a low concentration (20 mu g/L < AFP 400 <= mu g/L) in the other patients. The positive rate for at least one of the transcripts of three CIA genes in PBMC samples from the AFP negative or positive patients with a low concentration was 69.2% and 45.0%, respectively. Of the 90 patients, 71 (78.9%) were diagnosed as HCC by nested RT-PCR and serum AFP. Although the positive rate for at least one of the transcripts of three CIA genes in PBMC samples from 53 patients at TNM stage III or IV was obviously higher than that in PBMC samples from 37 patients at stage I or II (77.9% vs 51.4%, P = 0.010), the CIA mRNA was detected in 41.7% and 56.0% of PBMC samples from HCC patients at stages I and II, respectively.
CONCLUSION: Detecting MAGE-1, SSX-1 and CTp11 mRNA in PBMC improves the total diagnostic rate of HCC.
|资助机构||National Natural Science Foundation of China|
|作者单位||1.Peking Univ, Peoples Hosp, Ctr Hepatobiliary Surg, Beijing 100044, Peoples R China|
2.China Acad Chinese Med Sci, Wangjing Hosp, Dept Surg, Beijing 100102, Peoples R China
|Zhao, Li,Mou, Dong-Cheng,Peng, Ji-Run,et al. Diagnostic value of cancer-testis antigen mRNA in peripheral blood from hepatocellular carcinoma patients[J]. WORLD JOURNAL OF GASTROENTEROLOGY,2010,16(32):4072-4078.|
|APA||Zhao, Li,Mou, Dong-Cheng,Peng, Ji-Run,Huang, Lei,Wu, Zeng-An,&Leng, Xi-Sheng.(2010).Diagnostic value of cancer-testis antigen mRNA in peripheral blood from hepatocellular carcinoma patients.WORLD JOURNAL OF GASTROENTEROLOGY,16(32),4072-4078.|
|MLA||Zhao, Li,et al."Diagnostic value of cancer-testis antigen mRNA in peripheral blood from hepatocellular carcinoma patients".WORLD JOURNAL OF GASTROENTEROLOGY 16.32(2010):4072-4078.|