IR@PKUHSC  > 北京大学第二临床医学院  > 肝胆外科
学科主题临床医学
Diagnostic value of cancer-testis antigen mRNA in peripheral blood from hepatocellular carcinoma patients
Zhao, Li2; Mou, Dong-Cheng2; Peng, Ji-Run1; Huang, Lei1; Wu, Zeng-An2; Leng, Xi-Sheng1
关键词Hepatocellular carcinoma alpha-fetoprotein Cancer-testis antigen Diagnosis Nested reverse transcripts-polymerase chain reaction
刊名WORLD JOURNAL OF GASTROENTEROLOGY
2010-08-28
DOI10.3748/wjg.v16.i32.4072
16期:32页:4072-4078
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Gastroenterology & Hepatology
研究领域[WOS]Gastroenterology & Hepatology
关键词[WOS]POLYMERASE-CHAIN-REACTION ; CIRCULATING TUMOR-CELLS ; ALPHA-FETOPROTEIN ; SOLID TUMORS ; CANCER/TESTIS ANTIGENS ; REVERSE-TRANSCRIPTASE ; REACTION TECHNOLOGY ; BREAST-CANCER ; GENE FAMILY ; MICROMETASTASES
英文摘要

AIM: To evaluate the diagnostic value of cancer-testis antigen (CTA) mRNA in peripheral blood samples from hepatocellular carcinoma (HCC) patients.

METHODS: Peripheral blood samples were taken from 90 patients with HCC before operation. Expression of melanoma antigen-1 (MAGE-1), synovial sarcoma X break-point-1 (SSX-1), and cancer-testis-associated protein of 11 kDa (CTp11) mRNA in peripheral blood mononuclear cells (PBMC) was tested by nested reverse transcripts-polymerase chain reaction (RT-PCR). Serum a-fetoprotein (AFP) in these patients was also determined.

RESULTS: The positive rate of MAGE-1, SSX-1 and CTp11 transcripts was 37.7%, 34.4%, 31.1% in PBMC samples, and 74.4%, 73.3%, 62.2% in their resected tumor samples, respectively. The positive rate for at least one of the transcripts of three CTA genes was 66.7% in PBMC samples and 91.1% in their resected tumor samples. MAGE-1, SSX-1 and/or CTp11 mRNA were not detected in the PBMC of those patients from whom the resected tumor samples were MAGE-1, SSX-1 and/or CTp11 mRNA negative, nor in the PBMC samples from 20 healthy donors and 10 cirrhotic patients. Among the 90 patients, the serum AFP in 44 patients met the general diagnostic standard (AFP > 400 mu g/L) for HCC, and was negative (AFP <= 20 mu g/L) or positive with a low concentration (20 mu g/L < AFP 400 <= mu g/L) in the other patients. The positive rate for at least one of the transcripts of three CIA genes in PBMC samples from the AFP negative or positive patients with a low concentration was 69.2% and 45.0%, respectively. Of the 90 patients, 71 (78.9%) were diagnosed as HCC by nested RT-PCR and serum AFP. Although the positive rate for at least one of the transcripts of three CIA genes in PBMC samples from 53 patients at TNM stage III or IV was obviously higher than that in PBMC samples from 37 patients at stage I or II (77.9% vs 51.4%, P = 0.010), the CIA mRNA was detected in 41.7% and 56.0% of PBMC samples from HCC patients at stages I and II, respectively.

CONCLUSION: Detecting MAGE-1, SSX-1 and CTp11 mRNA in PBMC improves the total diagnostic rate of HCC.

语种英语
WOS记录号WOS:000281406200012
项目编号30200271
资助机构National Natural Science Foundation of China
引用统计
被引频次:7[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/67244
专题北京大学第二临床医学院_肝胆外科
作者单位1.Peking Univ, Peoples Hosp, Ctr Hepatobiliary Surg, Beijing 100044, Peoples R China
2.China Acad Chinese Med Sci, Wangjing Hosp, Dept Surg, Beijing 100102, Peoples R China
推荐引用方式
GB/T 7714
Zhao, Li,Mou, Dong-Cheng,Peng, Ji-Run,et al. Diagnostic value of cancer-testis antigen mRNA in peripheral blood from hepatocellular carcinoma patients[J]. WORLD JOURNAL OF GASTROENTEROLOGY,2010,16(32):4072-4078.
APA Zhao, Li,Mou, Dong-Cheng,Peng, Ji-Run,Huang, Lei,Wu, Zeng-An,&Leng, Xi-Sheng.(2010).Diagnostic value of cancer-testis antigen mRNA in peripheral blood from hepatocellular carcinoma patients.WORLD JOURNAL OF GASTROENTEROLOGY,16(32),4072-4078.
MLA Zhao, Li,et al."Diagnostic value of cancer-testis antigen mRNA in peripheral blood from hepatocellular carcinoma patients".WORLD JOURNAL OF GASTROENTEROLOGY 16.32(2010):4072-4078.
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