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Effects of U0126 on growth and activation of mitogen-activated protein kinases in Aspergillus fumigatus
Ma Dong-mei; Ji Ya-juan; Yang Fang; Liu Wei; Wan Zhe; Li Ruo-ya
关键词Aspergillus fumigatus protein kinase inhibitors mitogen-activated protein kinases
刊名CHINESE MEDICAL JOURNAL
2013-01-20
DOI10.3760/cma.j.issn.0366-6999.20121528
126期:2页:220-225
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Medicine, General & Internal
资助者National Foundation of Natural Sciences in China ; National Foundation of Natural Sciences in China
研究领域[WOS]General & Internal Medicine
关键词[WOS]CANCER CELL-LINES ; TRANSPLANT RECIPIENTS ; CANDIDA-ALBICANS ; REGULATED KINASE ; MAP KINASE ; SACCHAROMYCES-CEREVISIAE ; INVASIVE ASPERGILLOSIS ; FUNCTIONAL-ANALYSIS ; SIGNALING PATHWAY ; INHIBITOR U0126
英文摘要

Background Invasive aspergillosis (IA), which is mainly caused by Aspergillus fumigatus (A. fumigatus), is a major cause of morbidity and mortality in immunocompromised patients. Despite considerable progress in currently available antifungals the mortality still remains high in critically ill patients. U0126 which is a highly selective inhibitor of MEK1 and MEK2 in the RAF/MEK/ERK pathway in mammalian cells has been demonstrated to have an anti-proliferative role in cancer cells. The purpose of this study was to explore the role of U0126 on growth inhibition and activation of mitogen-activated protein kinases (MAPKs) in A. fumigatus.

Methods Germination percentage and hyphae growth in A. fumigatus treated with U0126 were observed and compared with untreated controls. Western blotting analysis was used to detect changes in activation of SakA, MpkA and MpkB.

Results U0126 inhibited germination and hyphae growth in A. fumigatus and enhanced the phosphorylation of SakA and MpkA under oxidative stress. U0126 at 10 mu mol/L did not block the activation of MpkB during nitrogen starvation stress.

Conclusion U0126 shows promise as an antifungal candidate and the MAPK pathway may be a possible antifungal drug target for A. fumigatus.

语种英语
所属项目编号30930006
资助者National Foundation of Natural Sciences in China ; National Foundation of Natural Sciences in China
WOS记录号WOS:000319632800005
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被引频次:1[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/67248
专题北京大学第一临床医学院_皮肤性病科
作者单位Peking Univ, Res Ctr Med Mycol, Hosp 1, Dept Dermatol, Beijing 100034, Peoples R China
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Ma Dong-mei,Ji Ya-juan,Yang Fang,et al. Effects of U0126 on growth and activation of mitogen-activated protein kinases in Aspergillus fumigatus[J]. CHINESE MEDICAL JOURNAL,2013,126(2):220-225.
APA Ma Dong-mei,Ji Ya-juan,Yang Fang,Liu Wei,Wan Zhe,&Li Ruo-ya.(2013).Effects of U0126 on growth and activation of mitogen-activated protein kinases in Aspergillus fumigatus.CHINESE MEDICAL JOURNAL,126(2),220-225.
MLA Ma Dong-mei,et al."Effects of U0126 on growth and activation of mitogen-activated protein kinases in Aspergillus fumigatus".CHINESE MEDICAL JOURNAL 126.2(2013):220-225.
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