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学科主题临床医学
Smad3 is the key to transforming growth factor-beta 1-induced osteoclast differentiation in giant cell tumor of bone
Lou, Zhiyuan; Yang, Yi; Ren, Tingting; Tang, Shun; Peng, Xianbo; Lu, Qunshan; Sun, Yifeng; Guo, Wei
关键词Giant cell tumor of bone Osteoclast differentiation Receptor activator of nuclear factor kappa B ligand Osteoprotegerin Immunohistochemistry Transforming growth factor-beta 1
刊名MEDICAL ONCOLOGY
2013-09-01
DOI10.1007/s12032-013-0606-8
30期:3
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Oncology
研究领域[WOS]Oncology
关键词[WOS]FACTOR-BETA ; OSTEOPROTEGERIN LIGAND ; POSSIBLE INVOLVEMENT ; RECEPTOR ACTIVATOR ; HUMAN OSTEOBLASTS ; GENE-EXPRESSION ; STROMAL CELL ; TGF-BETA ; KAPPA-B ; RANKL
英文摘要

Giant cell tumor (GCT) of bone is a benign but locally aggressive neoplasm of bone. However, molecular mechanisms underlying osteolysis in GCT have not been deeply understood. The aim of this study was to investigate one of the possible mechanisms underlying the up-regulation of receptor activator of nuclear factor kappa B ligand (RANKL)/osteoprotegerin (OPG) expression. First, we performed an immunohistochemical study on transforming growth factor-beta 1 (TGF-beta 1) expression in 83 cases with GCT and found that increased TGF-beta 1 staining was significantly correlated with Campanacci stages(Spearman′s correlation = 0.335, p = 0.002). Next, we investigated the mechanism of the effect of TGF-beta 1 on osteolysis of GCT and examined the effects of TGF-beta 1 plus or minus specific inhibitor of Smad3 (SIS3) on the expression of RANKL/OPG ratio at the mRNA and protein levels in two primary GCT cell lines. The results clearly indicated that TGF-beta 1 is capable of significantly increasing RANKL/OPG ratio (p(GCT1) = 0.000, p(GCT2) = 0.000) and that SIS3 is capable of reversing the ratio, suggesting that Smad3 is the key to TGF-beta 1-induced increased the ratio. In the co-culture system, we found that SIS3 reversed the effects of TGF-beta 1-induced osteoclast formation in the co-culture system (p(GCT1) = 0.000, p(GCT2) = 0.000). Our findings indicate that TGF-beta 1 plays an important role in the osteolysis of GCT via Smad3.

语种英语
WOS记录号WOS:000323662900087
Citation statistics
Cited Times:4[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/67261
Collection北京大学第二临床医学院_骨肿瘤科
北京大学药学院_天然药物与仿生药物国家重点实验室
作者单位Peking Univ, Peoples Hosp, Musculoskeletal Tumor Ctr, Beijing 100871, Peoples R China
Recommended Citation
GB/T 7714
Lou, Zhiyuan,Yang, Yi,Ren, Tingting,et al. Smad3 is the key to transforming growth factor-beta 1-induced osteoclast differentiation in giant cell tumor of bone[J]. MEDICAL ONCOLOGY,2013,30(3).
APA Lou, Zhiyuan.,Yang, Yi.,Ren, Tingting.,Tang, Shun.,Peng, Xianbo.,...&Guo, Wei.(2013).Smad3 is the key to transforming growth factor-beta 1-induced osteoclast differentiation in giant cell tumor of bone.MEDICAL ONCOLOGY,30(3).
MLA Lou, Zhiyuan,et al."Smad3 is the key to transforming growth factor-beta 1-induced osteoclast differentiation in giant cell tumor of bone".MEDICAL ONCOLOGY 30.3(2013).
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