IR@PKUHSC  > 北京大学第二临床医学院  > 骨肿瘤科
学科主题临床医学
Smad3 is the key to transforming growth factor-beta 1-induced osteoclast differentiation in giant cell tumor of bone
Lou, Zhiyuan; Yang, Yi; Ren, Tingting; Tang, Shun; Peng, Xianbo; Lu, Qunshan; Sun, Yifeng; Guo, Wei
关键词Giant cell tumor of bone Osteoclast differentiation Receptor activator of nuclear factor kappa B ligand Osteoprotegerin Immunohistochemistry Transforming growth factor-beta 1
刊名MEDICAL ONCOLOGY
2013-09-01
DOI10.1007/s12032-013-0606-8
30期:3
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Oncology
资助者National Natural Sciences Foundation of China ; National Natural Sciences Foundation of China
研究领域[WOS]Oncology
关键词[WOS]FACTOR-BETA ; OSTEOPROTEGERIN LIGAND ; POSSIBLE INVOLVEMENT ; RECEPTOR ACTIVATOR ; HUMAN OSTEOBLASTS ; GENE-EXPRESSION ; STROMAL CELL ; TGF-BETA ; KAPPA-B ; RANKL
英文摘要

Giant cell tumor (GCT) of bone is a benign but locally aggressive neoplasm of bone. However, molecular mechanisms underlying osteolysis in GCT have not been deeply understood. The aim of this study was to investigate one of the possible mechanisms underlying the up-regulation of receptor activator of nuclear factor kappa B ligand (RANKL)/osteoprotegerin (OPG) expression. First, we performed an immunohistochemical study on transforming growth factor-beta 1 (TGF-beta 1) expression in 83 cases with GCT and found that increased TGF-beta 1 staining was significantly correlated with Campanacci stages(Spearman′s correlation = 0.335, p = 0.002). Next, we investigated the mechanism of the effect of TGF-beta 1 on osteolysis of GCT and examined the effects of TGF-beta 1 plus or minus specific inhibitor of Smad3 (SIS3) on the expression of RANKL/OPG ratio at the mRNA and protein levels in two primary GCT cell lines. The results clearly indicated that TGF-beta 1 is capable of significantly increasing RANKL/OPG ratio (p(GCT1) = 0.000, p(GCT2) = 0.000) and that SIS3 is capable of reversing the ratio, suggesting that Smad3 is the key to TGF-beta 1-induced increased the ratio. In the co-culture system, we found that SIS3 reversed the effects of TGF-beta 1-induced osteoclast formation in the co-culture system (p(GCT1) = 0.000, p(GCT2) = 0.000). Our findings indicate that TGF-beta 1 plays an important role in the osteolysis of GCT via Smad3.

语种英语
所属项目编号81172543 ; 81001193
资助者National Natural Sciences Foundation of China ; National Natural Sciences Foundation of China
WOS记录号WOS:000323662900087
引用统计
被引频次:3[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/67261
专题北京大学第二临床医学院_骨肿瘤科
作者单位Peking Univ, Peoples Hosp, Musculoskeletal Tumor Ctr, Beijing 100871, Peoples R China
推荐引用方式
GB/T 7714
Lou, Zhiyuan,Yang, Yi,Ren, Tingting,et al. Smad3 is the key to transforming growth factor-beta 1-induced osteoclast differentiation in giant cell tumor of bone[J]. MEDICAL ONCOLOGY,2013,30(3).
APA Lou, Zhiyuan.,Yang, Yi.,Ren, Tingting.,Tang, Shun.,Peng, Xianbo.,...&Guo, Wei.(2013).Smad3 is the key to transforming growth factor-beta 1-induced osteoclast differentiation in giant cell tumor of bone.MEDICAL ONCOLOGY,30(3).
MLA Lou, Zhiyuan,et al."Smad3 is the key to transforming growth factor-beta 1-induced osteoclast differentiation in giant cell tumor of bone".MEDICAL ONCOLOGY 30.3(2013).
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Lou, Zhiyuan]的文章
[Yang, Yi]的文章
[Ren, Tingting]的文章
百度学术
百度学术中相似的文章
[Lou, Zhiyuan]的文章
[Yang, Yi]的文章
[Ren, Tingting]的文章
必应学术
必应学术中相似的文章
[Lou, Zhiyuan]的文章
[Yang, Yi]的文章
[Ren, Tingting]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。