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学科主题临床医学
Expression of human VEGF(121) cDNA in mouse bone marrow stromal cells
Cai, SB; Ma, QJ; Yu, XJ; Dang, GT; Ma, DL
关键词endothelial growth factor retrovirus vector gene therapy marrow stromal cell
刊名CHINESE MEDICAL JOURNAL
2002-06-01
115期:6页:914-918
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Medicine, General & Internal
研究领域[WOS]General & Internal Medicine
关键词[WOS]VASCULAR-PERMEABILITY FACTOR ; ENDOTHELIAL GROWTH-FACTOR ; LIMB ISCHEMIA ; ANGIOGENESIS ; GENE ; VEGF
英文摘要

Objective To construct a retroviral vector carrying human vascular endothelial growth factor ( hVEGF(121) cDNA for evaluation of the possibility of VEGF gene therapy in ischemic bone disease.

Methods hVEGF(121) cDNA was obtained from the plasmid pCDI/VEGF(121), and cloned into retroviral plasmid pLXSN. Recombinant plasmid was transferred to the retrovirus packaging cell, PT-67, by lipofectamine mediated gene transfer. Mouse bone marrow stromal cells (MSCs) were transfected by the retrovirus. The integration of the hVEGF(121) cDNA into MSC genomic DNA and expression of the VEGF gene was detected. Proliferation assays of human umbilical vein endothelial cells (HUVECs) by VEGF(121) in culture medium were performed.

Results Recombinant pLXSN/VEGF(121) was correctly constructed and confirmed by restriction endonuclease analysis and DNA sequencing analysis. hVEGF(121) gene was integrated into MSC genomic DNA after transfection, and the VEGF(121) protein was expressed. Proliferation assays showed VEGF(121) in culture medium was a biologically active protein and had a mitogenic effect on HUVEC.

Conclusions Recombinant retroviral vector carrying hVEGF(121) cDNA was successfully constructed. VEGF(121) protein expressed by MSCs had mitogenic effect biologically. This provides a further foundation for VEGF gene therapy for bone ischemic disease and bone tissue engineering.

语种英语
WOS记录号WOS:000176693300026
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被引频次:9[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/67288
专题北京大学第三临床医学院_骨科
作者单位1.Peking Univ, Dept Orthoped, Hosp 3, Beijing 100083, Peoples R China
2.Peking Univ, Ctr Human Dis Genom, Beijing 100083, Peoples R China
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GB/T 7714
Cai, SB,Ma, QJ,Yu, XJ,et al. Expression of human VEGF(121) cDNA in mouse bone marrow stromal cells[J]. CHINESE MEDICAL JOURNAL,2002,115(6):914-918.
APA Cai, SB,Ma, QJ,Yu, XJ,Dang, GT,&Ma, DL.(2002).Expression of human VEGF(121) cDNA in mouse bone marrow stromal cells.CHINESE MEDICAL JOURNAL,115(6),914-918.
MLA Cai, SB,et al."Expression of human VEGF(121) cDNA in mouse bone marrow stromal cells".CHINESE MEDICAL JOURNAL 115.6(2002):914-918.
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