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学科主题: 临床医学
题名:
Mutations in surface and polymerase gene of chronic hepatitis B patients with coexisting HBsAg and anti-HBs
作者: Lu, Hai-Ying; Zeng, Zheng; Xu, Xiao-Yuan; Zhang, Nai-Lin; Yu, Min; Gong, Wei-Bo
关键词: hepatitis B virus surface antigen ; antibody to hepatitis B virus surface ; mutation
刊名: WORLD JOURNAL OF GASTROENTEROLOGY
发表日期: 2006-07-14
卷: 12, 期:26, 页:4219-4223
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Gastroenterology & Hepatology
研究领域[WOS]: Gastroenterology & Hepatology
关键词[WOS]: LAMIVUDINE THERAPY ; VIRUS POLYMERASE ; INFECTION ; ESCAPE ; SELECTION ; VARIANTS ; EPITOPES ; ENVELOPE ; MUTANT
英文摘要:

AIM: To investigate the clinical significance and presence of mutations in the surface (S) and overlapping polymerase gene of hepatitis B patients with coexisting HBsAg and anti-HBs.

METHODS: Twenty-three patients with chronic hepatitis B were studied. Of the 23 patients, 11 were both positive for hepatitis B virus (HBV) surface antigen (HBsAg) and antibody to HBV surface antigen (anti-HBs), 12 were negative for anti-HBs while positive for HBsAg. DNA was extracted from 200 mu L serum of the patients. Nucleotide of the surface and overlapping polymerase gene from HBV-infected patients was amplified by PCR, and the PCR products were sequenced.

RESULTS: Forty-one mutations were found within the surface gene protein of HBV in 15 patients (10 with coexisting HBsAg and anti-HBs). Six (14.6%) out of 41 mutations were located at "alpha" determinant region in 5 patients (4 positive for HBsAg and anti-HBs). Eleven mutations (26.8%) occurred in the downstream or upstream of "alpha" determinant region. Lamivudine (LMV)-selected mutations were found in three patients who developed anti-HBs, which occurred in amino acid positions (196, 198, 199) of the surface protein and in YMDD motif (M204I/V) of the polymerase protein simultaneously. Presence of these mutations did not relate to changes in ALT and HBV DNA levels.

CONCLUSION: Besides mutations in the "a" determinant region, mutations at downstream or upstream of the "a" determinant region may contribute to the development of anti-HBs. These mutations do not block the replicating competency of HBV in the presence of high titer of anti-HBs. (c) 2006 The WIG Press. All rights reserved.

语种: 英语
WOS记录号: WOS:000239997800021
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/67291
Appears in Collections:北京大学第一临床医学院_感染疾病科_期刊论文

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作者单位: Peking Univ, Hosp 1, Dept Infect Dis, Beijing 100034, Peoples R China

Recommended Citation:
Lu, Hai-Ying,Zeng, Zheng,Xu, Xiao-Yuan,et al. Mutations in surface and polymerase gene of chronic hepatitis B patients with coexisting HBsAg and anti-HBs[J]. WORLD JOURNAL OF GASTROENTEROLOGY,2006,12(26):4219-4223.
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