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Mutations in surface and polymerase gene of chronic hepatitis B patients with coexisting HBsAg and anti-HBs
Lu, Hai-Ying; Zeng, Zheng; Xu, Xiao-Yuan; Zhang, Nai-Lin; Yu, Min; Gong, Wei-Bo
关键词hepatitis B virus surface antigen antibody to hepatitis B virus surface mutation
刊名WORLD JOURNAL OF GASTROENTEROLOGY
2006-07-14
12期:26页:4219-4223
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Gastroenterology & Hepatology
研究领域[WOS]Gastroenterology & Hepatology
关键词[WOS]LAMIVUDINE THERAPY ; VIRUS POLYMERASE ; INFECTION ; ESCAPE ; SELECTION ; VARIANTS ; EPITOPES ; ENVELOPE ; MUTANT
英文摘要

AIM: To investigate the clinical significance and presence of mutations in the surface (S) and overlapping polymerase gene of hepatitis B patients with coexisting HBsAg and anti-HBs.

METHODS: Twenty-three patients with chronic hepatitis B were studied. Of the 23 patients, 11 were both positive for hepatitis B virus (HBV) surface antigen (HBsAg) and antibody to HBV surface antigen (anti-HBs), 12 were negative for anti-HBs while positive for HBsAg. DNA was extracted from 200 mu L serum of the patients. Nucleotide of the surface and overlapping polymerase gene from HBV-infected patients was amplified by PCR, and the PCR products were sequenced.

RESULTS: Forty-one mutations were found within the surface gene protein of HBV in 15 patients (10 with coexisting HBsAg and anti-HBs). Six (14.6%) out of 41 mutations were located at "alpha" determinant region in 5 patients (4 positive for HBsAg and anti-HBs). Eleven mutations (26.8%) occurred in the downstream or upstream of "alpha" determinant region. Lamivudine (LMV)-selected mutations were found in three patients who developed anti-HBs, which occurred in amino acid positions (196, 198, 199) of the surface protein and in YMDD motif (M204I/V) of the polymerase protein simultaneously. Presence of these mutations did not relate to changes in ALT and HBV DNA levels.

CONCLUSION: Besides mutations in the "a" determinant region, mutations at downstream or upstream of the "a" determinant region may contribute to the development of anti-HBs. These mutations do not block the replicating competency of HBV in the presence of high titer of anti-HBs. (c) 2006 The WIG Press. All rights reserved.

语种英语
WOS记录号WOS:000239997800021
引用统计
被引频次:10[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/67291
专题北京大学第一临床医学院_感染疾病科
作者单位Peking Univ, Hosp 1, Dept Infect Dis, Beijing 100034, Peoples R China
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GB/T 7714
Lu, Hai-Ying,Zeng, Zheng,Xu, Xiao-Yuan,et al. Mutations in surface and polymerase gene of chronic hepatitis B patients with coexisting HBsAg and anti-HBs[J]. WORLD JOURNAL OF GASTROENTEROLOGY,2006,12(26):4219-4223.
APA Lu, Hai-Ying,Zeng, Zheng,Xu, Xiao-Yuan,Zhang, Nai-Lin,Yu, Min,&Gong, Wei-Bo.(2006).Mutations in surface and polymerase gene of chronic hepatitis B patients with coexisting HBsAg and anti-HBs.WORLD JOURNAL OF GASTROENTEROLOGY,12(26),4219-4223.
MLA Lu, Hai-Ying,et al."Mutations in surface and polymerase gene of chronic hepatitis B patients with coexisting HBsAg and anti-HBs".WORLD JOURNAL OF GASTROENTEROLOGY 12.26(2006):4219-4223.
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