|Mutations in surface and polymerase gene of chronic hepatitis B patients with coexisting HBsAg and anti-HBs|
|Lu, Hai-Ying; Zeng, Zheng; Xu, Xiao-Yuan; Zhang, Nai-Lin; Yu, Min; Gong, Wei-Bo|
|关键词||hepatitis B virus surface antigen antibody to hepatitis B virus surface mutation|
|刊名||WORLD JOURNAL OF GASTROENTEROLOGY|
|WOS标题词||Science & Technology|
|类目[WOS]||Gastroenterology & Hepatology|
|研究领域[WOS]||Gastroenterology & Hepatology|
|关键词[WOS]||LAMIVUDINE THERAPY ; VIRUS POLYMERASE ; INFECTION ; ESCAPE ; SELECTION ; VARIANTS ; EPITOPES ; ENVELOPE ; MUTANT|
AIM: To investigate the clinical significance and presence of mutations in the surface (S) and overlapping polymerase gene of hepatitis B patients with coexisting HBsAg and anti-HBs.
METHODS: Twenty-three patients with chronic hepatitis B were studied. Of the 23 patients, 11 were both positive for hepatitis B virus (HBV) surface antigen (HBsAg) and antibody to HBV surface antigen (anti-HBs), 12 were negative for anti-HBs while positive for HBsAg. DNA was extracted from 200 mu L serum of the patients. Nucleotide of the surface and overlapping polymerase gene from HBV-infected patients was amplified by PCR, and the PCR products were sequenced.
RESULTS: Forty-one mutations were found within the surface gene protein of HBV in 15 patients (10 with coexisting HBsAg and anti-HBs). Six (14.6%) out of 41 mutations were located at "alpha" determinant region in 5 patients (4 positive for HBsAg and anti-HBs). Eleven mutations (26.8%) occurred in the downstream or upstream of "alpha" determinant region. Lamivudine (LMV)-selected mutations were found in three patients who developed anti-HBs, which occurred in amino acid positions (196, 198, 199) of the surface protein and in YMDD motif (M204I/V) of the polymerase protein simultaneously. Presence of these mutations did not relate to changes in ALT and HBV DNA levels.
CONCLUSION: Besides mutations in the "a" determinant region, mutations at downstream or upstream of the "a" determinant region may contribute to the development of anti-HBs. These mutations do not block the replicating competency of HBV in the presence of high titer of anti-HBs. (c) 2006 The WIG Press. All rights reserved.
|作者单位||Peking Univ, Hosp 1, Dept Infect Dis, Beijing 100034, Peoples R China|
|Lu, Hai-Ying,Zeng, Zheng,Xu, Xiao-Yuan,et al. Mutations in surface and polymerase gene of chronic hepatitis B patients with coexisting HBsAg and anti-HBs[J]. WORLD JOURNAL OF GASTROENTEROLOGY,2006,12(26):4219-4223.|
|APA||Lu, Hai-Ying,Zeng, Zheng,Xu, Xiao-Yuan,Zhang, Nai-Lin,Yu, Min,&Gong, Wei-Bo.(2006).Mutations in surface and polymerase gene of chronic hepatitis B patients with coexisting HBsAg and anti-HBs.WORLD JOURNAL OF GASTROENTEROLOGY,12(26),4219-4223.|
|MLA||Lu, Hai-Ying,et al."Mutations in surface and polymerase gene of chronic hepatitis B patients with coexisting HBsAg and anti-HBs".WORLD JOURNAL OF GASTROENTEROLOGY 12.26(2006):4219-4223.|