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学科主题基础医学
Proteomic Analysis of Rat Hypothalamus Revealed the Role of Ubiquitin-Proteasome System in the Genesis of DR or DIO
Wang, Qi-ming1; Yang, Hui1; Tian, De-run1; Cai, Ying1; Wei, Zhong-nan1; Wang, Fei2; Yu, Albert Ch2; Han, Ji-sheng2
关键词Ubiquitin UCHL-1 Diet-induced obesity Diet-resistant Two-dimensional gel electrophoresis Mass spectrometry
刊名NEUROCHEMICAL RESEARCH
2011-06-01
DOI10.1007/s11064-011-0423-4
36期:6页:939-946
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Neurosciences
研究领域[WOS]Biochemistry & Molecular Biology ; Neurosciences & Neurology
关键词[WOS]DIET-INDUCED OBESE ; ENERGY-BALANCE ; PHYSICAL-ACTIVITY ; BODY-WEIGHT ; FOOD-INTAKE ; EXPRESSION ; MICE ; PROTEINS ; RECEPTOR ; PEPTIDE
英文摘要

Obesity has become a global epidemic, contributing to the increasing burdens of cardiovascular disease and type 2 diabetes. However, the precise molecular mechanisms of obesity remain poorly elucidated. The hypothalamus plays a major part in regulating energy homeostasis by integrating all kinds of nutritional signals. This study investigated the hypothalamus protein profile in diet-induced obese (DIO) and diet-resistant (DR) rats using two dimensional gel electrophoresis (2-DE) combined with MALDI-TOF/TOF-MS analysis. Twenty-two proteins were identified in the hypothalamus of DIO or DR rats. These include metabolic enzymes, antioxidant proteins, proteasome related proteins, and signaling proteins, some of which are related to AMP-activated protein kinase (AMPK) signaling or mitochondrial respiration. Among these proteins, in comparison with the normal-diet group, Ubiquitin was significantly decreased in DR rats but not changed in DIO rats, while Ubiquitin carboxyl-terminal esterase L1 (UCHL-1) was decreased in DIO rats but not changed in DR rats. The expression level of Ubiquitin and UCHL-1 were further validated using Western blot analysis. Our study reveals that Ubiquitin and UCHL-1 are obesity-related factors in the hypothalamus that may play an important role in the genesis of DR or DIO by interfering with the integrated signaling network that control energy balance and feeding.

语种英语
WOS记录号WOS:000290034000002
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被引频次:4[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/67294
专题北京大学基础医学院_神经生物学系
北京大学第三临床医学院_特需病房
作者单位1.Peking Univ, Neurosci Res Inst, Beijing 100083, Peoples R China
2.Tianjin Med Univ, Dept Anat, Tianjin 300070, Peoples R China
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Wang, Qi-ming,Yang, Hui,Tian, De-run,et al. Proteomic Analysis of Rat Hypothalamus Revealed the Role of Ubiquitin-Proteasome System in the Genesis of DR or DIO[J]. NEUROCHEMICAL RESEARCH,2011,36(6):939-946.
APA Wang, Qi-ming.,Yang, Hui.,Tian, De-run.,Cai, Ying.,Wei, Zhong-nan.,...&Han, Ji-sheng.(2011).Proteomic Analysis of Rat Hypothalamus Revealed the Role of Ubiquitin-Proteasome System in the Genesis of DR or DIO.NEUROCHEMICAL RESEARCH,36(6),939-946.
MLA Wang, Qi-ming,et al."Proteomic Analysis of Rat Hypothalamus Revealed the Role of Ubiquitin-Proteasome System in the Genesis of DR or DIO".NEUROCHEMICAL RESEARCH 36.6(2011):939-946.
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