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学科主题: 基础医学
题名:
Proteomic Analysis of Rat Hypothalamus Revealed the Role of Ubiquitin-Proteasome System in the Genesis of DR or DIO
作者: Wang, Qi-ming1; Yang, Hui1; Tian, De-run1; Cai, Ying1; Wei, Zhong-nan1; Wang, Fei2; Yu, Albert Ch2; Han, Ji-sheng2
关键词: Ubiquitin ; UCHL-1 ; Diet-induced obesity ; Diet-resistant ; Two-dimensional gel electrophoresis ; Mass spectrometry
刊名: NEUROCHEMICAL RESEARCH
发表日期: 2011-06-01
DOI: 10.1007/s11064-011-0423-4
卷: 36, 期:6, 页:939-946
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology ; Neurosciences
研究领域[WOS]: Biochemistry & Molecular Biology ; Neurosciences & Neurology
关键词[WOS]: DIET-INDUCED OBESE ; ENERGY-BALANCE ; PHYSICAL-ACTIVITY ; BODY-WEIGHT ; FOOD-INTAKE ; EXPRESSION ; MICE ; PROTEINS ; RECEPTOR ; PEPTIDE
英文摘要:

Obesity has become a global epidemic, contributing to the increasing burdens of cardiovascular disease and type 2 diabetes. However, the precise molecular mechanisms of obesity remain poorly elucidated. The hypothalamus plays a major part in regulating energy homeostasis by integrating all kinds of nutritional signals. This study investigated the hypothalamus protein profile in diet-induced obese (DIO) and diet-resistant (DR) rats using two dimensional gel electrophoresis (2-DE) combined with MALDI-TOF/TOF-MS analysis. Twenty-two proteins were identified in the hypothalamus of DIO or DR rats. These include metabolic enzymes, antioxidant proteins, proteasome related proteins, and signaling proteins, some of which are related to AMP-activated protein kinase (AMPK) signaling or mitochondrial respiration. Among these proteins, in comparison with the normal-diet group, Ubiquitin was significantly decreased in DR rats but not changed in DIO rats, while Ubiquitin carboxyl-terminal esterase L1 (UCHL-1) was decreased in DIO rats but not changed in DR rats. The expression level of Ubiquitin and UCHL-1 were further validated using Western blot analysis. Our study reveals that Ubiquitin and UCHL-1 are obesity-related factors in the hypothalamus that may play an important role in the genesis of DR or DIO by interfering with the integrated signaling network that control energy balance and feeding.

语种: 英语
所属项目编号: 3087791 ; 07JCDJC08100
项目资助者: National Natural Science Foundation of China ; Natural Science Foundation of Tianjin
WOS记录号: WOS:000290034000002
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/67294
Appears in Collections:基础医学院_神经生物学系_期刊论文

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作者单位: 1.Peking Univ, Neurosci Res Inst, Beijing 100083, Peoples R China
2.Tianjin Med Univ, Dept Anat, Tianjin 300070, Peoples R China

Recommended Citation:
Wang, Qi-ming,Yang, Hui,Tian, De-run,et al. Proteomic Analysis of Rat Hypothalamus Revealed the Role of Ubiquitin-Proteasome System in the Genesis of DR or DIO[J]. NEUROCHEMICAL RESEARCH,2011,36(6):939-946.
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