IR@PKUHSC  > 北京大学第三临床医学院  > 运动医学研究所
学科主题临床医学
Sonic Hedgehog Improves Redifferentiation of Dedifferentiated Chondrocytes for Articular Cartilage Repair
Lin, Lin1; Shen, Qi2; Xue, Tao1; Duan, Xiaoning1; Fu, Xin1; Yu, Changlong1
刊名PLOS ONE
2014-02-12
DOI10.1371/journal.pone.0088550
9期:2
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Multidisciplinary Sciences
研究领域[WOS]Science & Technology - Other Topics
关键词[WOS]HUMAN OSTEOARTHRITIC CARTILAGE ; CHONDROGENIC PROGENITOR CELLS ; LONG-TERM CULTURE ; SOMITIC CHONDROGENESIS ; FEEDBACK LOOP ; LIMB BUD ; COLLAGEN ; GROWTH ; EXPRESSION ; PROTEIN
英文摘要

Sonic hedgehog (Shh) is involved in the induction of early cartilaginous differentiation of mesenchymal cells in the limb. We investigated whether Shh could promote redifferentiation of dedifferentiated chondrocytes and have a favorable effect on the regeneration of cartilage. Articular chondrocytes of rats were separated and cultured. The redifferentiation of dedifferentiated chondrocytes transfected with Shh was evaluated using monolayer and pellet culture system. The signaling molecules (Ptc 1, Gli 1 and Sox9) of the hedgehog pathway were investigated. A rat model of articular cartilage defect was used to evaluate cartilage repair after transplantation with dedifferentiated chondrocytes. After Shh gene transfer, the hedgehog pathway was upregulated in dedifferentiated chondrocytes. Real time-PCR and western blot analysis verified the stronger expression of Ptc1, Gli1 and Sox9 in Shh transfected cells. Shh upregulates the Shh signaling pathway and multiple cytokines (bone morphogenetic protein 2 and insulin-like growth factor 1) in dedifferentiated chondrocytes. After transplantation in the joint, histologic analysis of the regenerative tissues revealed that significantly better cartilage repair in rats transplanted with Shh transfected cells. These data suggest that Shh could induce redifferentiation of dedifferentiated chondrocytes through up-regulating Shh signaling pathway, and have considerable therapeutic potential in cartilage repair.

语种英语
WOS记录号WOS:000331262600063
项目编号30900711 ; 2008B04
资助机构National Natural Scientific Foundation of China ; Beijing NOVA Program
引用统计
被引频次:12[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/67305
专题北京大学第三临床医学院_运动医学研究所
北京大学精神卫生研究所_护理部
作者单位1.Peking Univ, Hosp 1, Inst Urol, Beijing 100871, Peoples R China
2.Peking Univ, Hosp 3, Inst Sports Med, Beijing 100871, Peoples R China
推荐引用方式
GB/T 7714
Lin, Lin,Shen, Qi,Xue, Tao,et al. Sonic Hedgehog Improves Redifferentiation of Dedifferentiated Chondrocytes for Articular Cartilage Repair[J]. PLOS ONE,2014,9(2).
APA Lin, Lin,Shen, Qi,Xue, Tao,Duan, Xiaoning,Fu, Xin,&Yu, Changlong.(2014).Sonic Hedgehog Improves Redifferentiation of Dedifferentiated Chondrocytes for Articular Cartilage Repair.PLOS ONE,9(2).
MLA Lin, Lin,et al."Sonic Hedgehog Improves Redifferentiation of Dedifferentiated Chondrocytes for Articular Cartilage Repair".PLOS ONE 9.2(2014).
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