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学科主题: 临床医学
题名:
Sonic Hedgehog Improves Redifferentiation of Dedifferentiated Chondrocytes for Articular Cartilage Repair
作者: Lin, Lin1; Shen, Qi2; Xue, Tao1; Duan, Xiaoning1; Fu, Xin1; Yu, Changlong1
刊名: PLOS ONE
发表日期: 2014-02-12
DOI: 10.1371/journal.pone.0088550
卷: 9, 期:2
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Multidisciplinary Sciences
研究领域[WOS]: Science & Technology - Other Topics
关键词[WOS]: HUMAN OSTEOARTHRITIC CARTILAGE ; CHONDROGENIC PROGENITOR CELLS ; LONG-TERM CULTURE ; SOMITIC CHONDROGENESIS ; FEEDBACK LOOP ; LIMB BUD ; COLLAGEN ; GROWTH ; EXPRESSION ; PROTEIN
英文摘要:

Sonic hedgehog (Shh) is involved in the induction of early cartilaginous differentiation of mesenchymal cells in the limb. We investigated whether Shh could promote redifferentiation of dedifferentiated chondrocytes and have a favorable effect on the regeneration of cartilage. Articular chondrocytes of rats were separated and cultured. The redifferentiation of dedifferentiated chondrocytes transfected with Shh was evaluated using monolayer and pellet culture system. The signaling molecules (Ptc 1, Gli 1 and Sox9) of the hedgehog pathway were investigated. A rat model of articular cartilage defect was used to evaluate cartilage repair after transplantation with dedifferentiated chondrocytes. After Shh gene transfer, the hedgehog pathway was upregulated in dedifferentiated chondrocytes. Real time-PCR and western blot analysis verified the stronger expression of Ptc1, Gli1 and Sox9 in Shh transfected cells. Shh upregulates the Shh signaling pathway and multiple cytokines (bone morphogenetic protein 2 and insulin-like growth factor 1) in dedifferentiated chondrocytes. After transplantation in the joint, histologic analysis of the regenerative tissues revealed that significantly better cartilage repair in rats transplanted with Shh transfected cells. These data suggest that Shh could induce redifferentiation of dedifferentiated chondrocytes through up-regulating Shh signaling pathway, and have considerable therapeutic potential in cartilage repair.

语种: 英语
所属项目编号: 30900711 ; 2008B04
项目资助者: National Natural Scientific Foundation of China ; Beijing NOVA Program
WOS记录号: WOS:000331262600063
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/67305
Appears in Collections:北京大学第三临床医学院_运动医学研究所_期刊论文

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作者单位: 1.Peking Univ, Hosp 1, Inst Urol, Beijing 100871, Peoples R China
2.Peking Univ, Hosp 3, Inst Sports Med, Beijing 100871, Peoples R China

Recommended Citation:
Lin, Lin,Shen, Qi,Xue, Tao,et al. Sonic Hedgehog Improves Redifferentiation of Dedifferentiated Chondrocytes for Articular Cartilage Repair[J]. PLOS ONE,2014,9(2).
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