IR@PKUHSC  > 北京大学口腔医学院
学科主题口腔医学
Proliferation and Osteogenic Differentiation of Mesenchymal Stem Cells Induced by a Short Isoform of NELL-1
Pang, Shen1,2; Shen, Jia3,4; Liu, Yi3,4,7,8; Chen, Feng3,4,9; Zheng, Zhong3,4; James, Aaron W.1,2,3,4,5; Hsu, Ching-Yun3,4; Zhang, Hong3,4,10; Lee, Kevin S.3,4; Wang, Chunling7,8; Li, Chenshuang3,4; Chen, Xuepeng1,2; Jia, Haichao3,4,11; Zhang, Xinli3,4; Soo, Chia1,2,6; Ting, Kang3,4
关键词MSC proliferation NELL-1 Short NELL-1 isoform Osteogenesis Secretory protein
刊名STEM CELLS
2015-03-01
DOI10.1002/stem.1884
33期:3页:904-915
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Cell & Tissue Engineering ; Biotechnology & Applied Microbiology ; Oncology ; Cell Biology ; Hematology
研究领域[WOS]Cell Biology ; Biotechnology & Applied Microbiology ; Oncology ; Hematology
关键词[WOS]MARROW STROMAL CELLS ; SPINAL-FUSION MODEL ; CALVARIAL DEFECTS ; BONE-FORMATION ; IN-VITRO ; OSTEOBLAST DIFFERENTIATION ; MORPHOGENETIC PROTEIN-2 ; RECEPTOR-GAMMA ; GROWTH-FACTOR ; NOTCH
英文摘要

Neural epidermal growth factor-like (NEL)-like protein 1 (NELL-1) has been identified as an osteoinductive differentiation factor that promotes mesenchymal stem cell (MSC) osteogenic differentiation. In addition to full-length NELL-1, there are several NELL-1-related transcripts reported. We used rapid amplification of cDNA ends to recover potential cDNA of NELL-1 isoforms. A NELL-1 isoform with the N-terminal 240 amino acid (aa) residues truncated was identified. While full-length NELL-1 that contains 810 aa residues (NELL-1(810)) plays an important role in embryologic skeletal development, the N-terminal-truncated NELL-1 isoform (NELL-1(570)) was expressed postnatally. Similar to NELL-1(810), NELL-1(570) induced MSC osteogenic differentiation. In addition, NELL-1(570) significantly stimulated MSC proliferation in multiple MSC-like populations such as murine C3H10T1/2 MSC cell line, mouse primary MSCs, and perivascular stem cells, which is a type of stem cells proposed as the perivascular origin of MSCs. In contrast, NELL-1(810) demonstrated only limited stimulation of MSC proliferation. Similar to NELL-1(810), NELL-1(570) was found to be secreted from host cells. Both NELL-1(570) expression lentiviral vector and column-purified recombinant protein NELL-1(570) demonstrated almost identical effects in MSC proliferation and osteogenic differentiation, suggesting that NELL-1(570) may function as a pro-osteogenic growth factor. In vivo, NELL-1(570) induced significant calvarial defect regeneration accompanied by increased cell proliferation. Thus, NELL-1(570) has the potential to be used for cell-based or hormone-based therapy of bone regeneration.

语种英语
WOS记录号WOS:000349849000024
引用统计
被引频次:9[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/67327
专题北京大学口腔医学院
作者单位1.Peking Univ, Cent Lab, Sch Stomatol, Beijing 100871, Peoples R China
2.Capital Med Univ, Sch Stomatol, Dept Orthodont, Beijing, Peoples R China
3.Shandong Univ, Sch & Hosp Stomatol, UCLA & Orthopaed Hosp, Dept Orthopaed Surg, Jinan, Shandong, Peoples R China
4.Shandong Univ, Sch & Hosp Stomatol, Orthopaed Hosp Res Ctr, Jinan, Shandong, Peoples R China
5.Shandong Univ, Sch & Hosp Stomatol, Dent & Craniofacial Res Inst, Jinan, Shandong, Peoples R China
6.Shandong Univ, Sch & Hosp Stomatol, Sect Orthodont, Sch Dent, Jinan, Shandong, Peoples R China
7.Shandong Univ, Sch & Hosp Stomatol, Dept Pathol & Lab Med, David Geffen Sch Med, Jinan, Shandong, Peoples R China
8.Shandong Univ, Sch & Hosp Stomatol, Dept Surg, Div Plast & Reconstruct Surg, Jinan, Shandong, Peoples R China
9.Shandong Univ, Sch & Hosp Stomatol, Shandong Prov Lab Oral Biomed, Jinan, Shandong, Peoples R China
10.Shandong Univ, Sch & Hosp Stomatol, Dept Orthodont, Jinan, Shandong, Peoples R China
11.Sun Yat Sen Univ, Guanghua Sch Stomatol, Dept Orthodont, Guangzhou 510275, Guangdong, Peoples R China
推荐引用方式
GB/T 7714
Pang, Shen,Shen, Jia,Liu, Yi,et al. Proliferation and Osteogenic Differentiation of Mesenchymal Stem Cells Induced by a Short Isoform of NELL-1[J]. STEM CELLS,2015,33(3):904-915.
APA Pang, Shen.,Shen, Jia.,Liu, Yi.,Chen, Feng.,Zheng, Zhong.,...&Ting, Kang.(2015).Proliferation and Osteogenic Differentiation of Mesenchymal Stem Cells Induced by a Short Isoform of NELL-1.STEM CELLS,33(3),904-915.
MLA Pang, Shen,et al."Proliferation and Osteogenic Differentiation of Mesenchymal Stem Cells Induced by a Short Isoform of NELL-1".STEM CELLS 33.3(2015):904-915.
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