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IR@PKUHSC  > 北京大学第三临床医学院  > 心血管内科  > 期刊论文
学科主题: 临床医学
题名:
Comparison of zonal elution and nonlinear chromatography in determination of the interaction between seven drugs and immobilised beta(2)-adrenoceptor
作者: Li, Qian1; Wang, Jing1; Zheng, Yuqing Yuan2; Yang, Lingjian1; Zhang, Yajun1; Bian, Liujiao1; Zheng, Jianbin1; Li, Zijian3,4; Zhao, Xinfeng1; Zhang, Youyi3,4
关键词: Affinity chromatography ; Zonal elution ; Nonlinear chromatography ; Drug-protein interaction ; beta(2)-Adrenoceptor
刊名: JOURNAL OF CHROMATOGRAPHY A
发表日期: 2015-07-03
DOI: 10.1016/j.chroma.2015.05.012
卷: 1401, 页:75-83
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]: PERFORMANCE AFFINITY-CHROMATOGRAPHY ; HUMAN SERUM-ALBUMIN ; SMALL MOLECULE INTERACTIONS ; SURFACE-PLASMON RESONANCE ; PROTEIN-COUPLED RECEPTOR ; LIGAND BINDING-AFFINITY ; LIQUID-CHROMATOGRAPHY ; NONCOMPETITIVE INHIBITORS ; CONFORMATIONAL MOBILITY ; STATIONARY PHASES
英文摘要:

Zonal elution and nonlinear chromatography are two mainstream models for the determination of drug-protein interaction in affinity chromatography. This work intended to compare the results by zonal elution with that by nonlinear chromatography when it comes to the analysis of the interaction between seven drugs and immobilised beta(2)-adrenoceptor (beta(2)-AR). The results of the zonal elution showed that clorprenaline, clenbuterol, methoxyphenamine, salbutamol, terbutaline, tulobuterol and bambuterol have only one type of binding site on immobilised beta(2)-AR, while nonlinear chromatography confirmed the existence of at least two types of binding sites between beta(2)-AR and clorprenaline, clenbuterol and bambuterol. On these sites, both zonal elution and nonlinear chromatography presented the same rank order for the association constants of the seven drugs. Compared with the data from zonal elution, the association constants calculated using nonlinear chromatography gave a good linear response to the corresponding values by radio-ligand binding assay. The sampling efficiencies of nonlinear chromatography were clearly higher than zonal elution. Nonlinear chromatography will probably become a powerful alternative for the high throughput determination of drug-protein interaction. (C) 2015 Elsevier B.V. All rights reserved.

语种: 英语
所属项目编号: 21475103 ; IRT1174 ; 2013KCT-24 ; 2013YQ170525 ; 2013YQ17052509
项目资助者: National Natural Science Foundation of China ; Program for Changjiang Scholars and Innovative Research Team in University ; Project for Innovative Research Team of Research and Technology of Shaanxi Province ; National Key Scientific Instrument and Equipment Development Project of China
WOS记录号: WOS:000356550800010
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/67338
Appears in Collections:北京大学第三临床医学院_心血管内科_期刊论文

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作者单位: 1.Yanan Univ, Xian Innovat Coll, Xian 710100, Peoples R China
2.Minist Educ, Key Lab Mol Cardiovasc Sci, Beijing 100083, Peoples R China
3.NW Univ Xian, Coll Life Sci, Minist Educ, Key Lab Resource Biol & Biotechnol Western China, Xian 710069, Peoples R China
4.Peking Univ, Hosp 3, Inst Vasc Med, Beijing 100083, Peoples R China

Recommended Citation:
Li, Qian,Wang, Jing,Zheng, Yuqing Yuan,et al. Comparison of zonal elution and nonlinear chromatography in determination of the interaction between seven drugs and immobilised beta(2)-adrenoceptor[J]. JOURNAL OF CHROMATOGRAPHY A,2015,1401:75-83.
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