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学科主题临床医学
Comparison of zonal elution and nonlinear chromatography in determination of the interaction between seven drugs and immobilised beta(2)-adrenoceptor
Li, Qian1; Wang, Jing1; Zheng, Yuqing Yuan2; Yang, Lingjian1; Zhang, Yajun1; Bian, Liujiao1; Zheng, Jianbin1; Li, Zijian3,4; Zhao, Xinfeng1; Zhang, Youyi3,4
关键词Affinity chromatography Zonal elution Nonlinear chromatography Drug-protein interaction beta(2)-Adrenoceptor
刊名JOURNAL OF CHROMATOGRAPHY A
2015-07-03
DOI10.1016/j.chroma.2015.05.012
1401页:75-83
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]PERFORMANCE AFFINITY-CHROMATOGRAPHY ; HUMAN SERUM-ALBUMIN ; SMALL MOLECULE INTERACTIONS ; SURFACE-PLASMON RESONANCE ; PROTEIN-COUPLED RECEPTOR ; LIGAND BINDING-AFFINITY ; LIQUID-CHROMATOGRAPHY ; NONCOMPETITIVE INHIBITORS ; CONFORMATIONAL MOBILITY ; STATIONARY PHASES
英文摘要

Zonal elution and nonlinear chromatography are two mainstream models for the determination of drug-protein interaction in affinity chromatography. This work intended to compare the results by zonal elution with that by nonlinear chromatography when it comes to the analysis of the interaction between seven drugs and immobilised beta(2)-adrenoceptor (beta(2)-AR). The results of the zonal elution showed that clorprenaline, clenbuterol, methoxyphenamine, salbutamol, terbutaline, tulobuterol and bambuterol have only one type of binding site on immobilised beta(2)-AR, while nonlinear chromatography confirmed the existence of at least two types of binding sites between beta(2)-AR and clorprenaline, clenbuterol and bambuterol. On these sites, both zonal elution and nonlinear chromatography presented the same rank order for the association constants of the seven drugs. Compared with the data from zonal elution, the association constants calculated using nonlinear chromatography gave a good linear response to the corresponding values by radio-ligand binding assay. The sampling efficiencies of nonlinear chromatography were clearly higher than zonal elution. Nonlinear chromatography will probably become a powerful alternative for the high throughput determination of drug-protein interaction. (C) 2015 Elsevier B.V. All rights reserved.

语种英语
WOS记录号WOS:000356550800010
项目编号21475103 ; IRT1174 ; 2013KCT-24 ; 2013YQ170525 ; 2013YQ17052509
资助机构National Natural Science Foundation of China ; Program for Changjiang Scholars and Innovative Research Team in University ; Project for Innovative Research Team of Research and Technology of Shaanxi Province ; National Key Scientific Instrument and Equipment Development Project of China
引用统计
被引频次:6[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/67338
专题北京大学第三临床医学院_心血管内科
作者单位1.Yanan Univ, Xian Innovat Coll, Xian 710100, Peoples R China
2.Minist Educ, Key Lab Mol Cardiovasc Sci, Beijing 100083, Peoples R China
3.NW Univ Xian, Coll Life Sci, Minist Educ, Key Lab Resource Biol & Biotechnol Western China, Xian 710069, Peoples R China
4.Peking Univ, Hosp 3, Inst Vasc Med, Beijing 100083, Peoples R China
推荐引用方式
GB/T 7714
Li, Qian,Wang, Jing,Zheng, Yuqing Yuan,et al. Comparison of zonal elution and nonlinear chromatography in determination of the interaction between seven drugs and immobilised beta(2)-adrenoceptor[J]. JOURNAL OF CHROMATOGRAPHY A,2015,1401:75-83.
APA Li, Qian.,Wang, Jing.,Zheng, Yuqing Yuan.,Yang, Lingjian.,Zhang, Yajun.,...&Zhang, Youyi.(2015).Comparison of zonal elution and nonlinear chromatography in determination of the interaction between seven drugs and immobilised beta(2)-adrenoceptor.JOURNAL OF CHROMATOGRAPHY A,1401,75-83.
MLA Li, Qian,et al."Comparison of zonal elution and nonlinear chromatography in determination of the interaction between seven drugs and immobilised beta(2)-adrenoceptor".JOURNAL OF CHROMATOGRAPHY A 1401(2015):75-83.
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