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Transport of nerve growth factor encapsulated into liposomes across the blood-brain barrier: In vitro and in vivo studies
Xie, Y; Ye, LY; Zhang, XB; Cui, W; Lou, JN; Nagai, T; Hou, XP
关键词NGF RMP-7 liposomes in vitro BBB model in vitro and in vivo studies
刊名JOURNAL OF CONTROLLED RELEASE
2005-06-20
DOI10.1016/j.jconrel.2005.03.005
105期:1-2页:106-119
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Chemistry, Multidisciplinary ; Pharmacology & Pharmacy
研究领域[WOS]Chemistry ; Pharmacology & Pharmacy
关键词[WOS]MICROVASCULAR ENDOTHELIAL-CELLS ; DRUG-DELIVERY ; RAT-BRAIN ; NEUROTROPHIC FACTORS ; SPINAL-CORD ; PERMEABILITY ; RMP-7 ; CIRCULATION ; MODULATION
英文摘要

A nerve growth factor (NGF) was encapsulated into liposomes in order to protect it from the enzyme degradation in vivo and promote it permeability across the blood-brain barrier (BBB). RMP-7, a ligand to the B2 receptor on brain microvascular endothelial cells (BMVEC), was combined with 1,2-dioleoyl-sn-glycero-3-phosphoetbanolamine-n-[poly(ethylenegly-col)]hydroxy succinamide (DSPE-PEG-NHS) to obtain DSPE-PEG-RMP-7. Then DSPE-PEG-RMP-7 was incorporated into the liposomes′ surface to target sterically stabilized liposomes (SSL-T) to the brain. The highest percent of NGF encapsulated into liposomes was about 34%, and the average size of liposomes was below 100 nm. A primary model of 131313 was established and evaluated by morphological, permeability, and transendothelial electrical resistance (TEER). The BBB model was employed to study the permeability of NGF liposomes in vitro. The results indicated that the liposomes could enhance transport of NGF across the BBB. The best transport rate was received with NGF-SSL-T. The brain distribution of NGF liposomes was studied in vivo, the amount of NGF in the brain was increased in the order: NGF-SSL-T > NGF-SSL+RMP-7 > NGF-SSL > NGF-L. The maximum concentration of NGF was recorded in 30 min following the intravenous injection. In particular, a majority of NGF was distributed in striatum, hippocampus and cortex, and the concentration of NGF was relatively lower in olfactory bulb, cerebellum and brain stem. There was a close relationship between P-e (permeability coefficient on in vitro BBB model) and T, (brain targeted coefficient in vivo) for NGF encapsulated into the liposomes. (c) 2005 Elsevier B.V. All rights reserved.

语种英语
WOS记录号WOS:000230576600010
引用统计
被引频次:101[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/67342
专题北京大学药学院
作者单位1.Peking Univ, Sch Pharmaceut Sci, Dept Phys Pharm, Beijing 100083, Peoples R China
2.China Japan Friendship Hosp, Inst Clin Med Sci, Minist Hlth, Beijing 100029, Peoples R China
3.Hoshi Univ, Dept Pharmaceut Fac, Tokyo 142, Japan
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GB/T 7714
Xie, Y,Ye, LY,Zhang, XB,et al. Transport of nerve growth factor encapsulated into liposomes across the blood-brain barrier: In vitro and in vivo studies[J]. JOURNAL OF CONTROLLED RELEASE,2005,105(1-2):106-119.
APA Xie, Y.,Ye, LY.,Zhang, XB.,Cui, W.,Lou, JN.,...&Hou, XP.(2005).Transport of nerve growth factor encapsulated into liposomes across the blood-brain barrier: In vitro and in vivo studies.JOURNAL OF CONTROLLED RELEASE,105(1-2),106-119.
MLA Xie, Y,et al."Transport of nerve growth factor encapsulated into liposomes across the blood-brain barrier: In vitro and in vivo studies".JOURNAL OF CONTROLLED RELEASE 105.1-2(2005):106-119.
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