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学科主题临床医学
Calibration and standardisation of graded optic nerve injuries in rats
Huang, Hou-Bin1; Zhang, Yi-Xin1; Zhang, Shui-Hua2; Ma, Zhi-Zhong3
关键词ON injuries Animal model Retinal ganglion cell Optic nerve axon Rats
刊名NEUROLOGICAL RESEARCH
2015-07-01
DOI10.1179/1743132815Y.0000000014
37期:7页:638-649
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Clinical Neurology ; Neurosciences
资助者National Natural Science Foundation of China ; National Natural Science Foundation of China
研究领域[WOS]Neurosciences & Neurology
关键词[WOS]RETINAL GANGLION-CELLS ; AXONAL INJURY ; SECONDARY DEGENERATION ; EXPERIMENTAL GLAUCOMA ; EXPERIMENTAL-MODEL ; ADULT-RATS ; CRUSH ; TRANSECTION ; REGENERATION ; SURVIVAL
英文摘要

Objectives: To calibrate and standardise an animal model of graded optic nerve injury (ONI) in rats to facilitate future inter-laboratory data comparisons, focussing on quantification of injury intensity, injury severity, and the correlation between them.

Methods: A pair of cross-action forceps or a pair of artery clips was used to induce optic nerve (ON) crush injuries. A lever principle and a simplified method were used to measure the crushing force. The simplified method directly measured weights as an external force exerted on the tip of the forceps or clips, which was just sufficient to maintain a gap and was equivalent to the closing (crush) force. The impulse and averaged impulse were explored as physical quantities to compare injury intensities. Graded ONIs were made by crushing the ON for 3, 6, 12, 30 or 60 seconds by the cross-action forceps, or 5, 10 or 15 seconds by the artery clips. The injury severity was evaluated by counting surviving retinal ganglion cell (RGC) through applied FluoroGold to the superior colliculus and lateral geniculate body before ON crush, intact RGC counting by applied FluoroGold after ON crush, and ON axon counting.

Results: Similar results were obtained by the lever principle method and the simplified method. The crushing force of the cross-action forceps and the artery clips was 148.0 gram force (gf) and 32.4 gf, respectively. The graded ONI animal models were successfully created in rats without retinal ischaemia post-trauma. The averaged impulse produced by the artery clips for 15 seconds was equal to that produced by a 3-second crush of the cross-action forceps. The correlation between injury intensity and injury severity was fitted for a power function.

Discussion: Our results provide a simplified and effective means to quantify and analyse data from ON crush studies compared with previously reported animal models.

语种英语
所属项目编号30271384
资助者National Natural Science Foundation of China ; National Natural Science Foundation of China
WOS记录号WOS:000355659500011
引用统计
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/67350
专题北京大学第三临床医学院_眼科
作者单位1.Gen Hosp PLA, Hainan Branch, Dept Ophthalmol, Sanya, Peoples R China
2.Haerbin Med Univ, Affiliated Hosp 2, Dept Ophthalmol, Haerbin, Peoples R China
3.Peking Univ, Ctr Eye, Beijing 100083, Peoples R China
推荐引用方式
GB/T 7714
Huang, Hou-Bin,Zhang, Yi-Xin,Zhang, Shui-Hua,et al. Calibration and standardisation of graded optic nerve injuries in rats[J]. NEUROLOGICAL RESEARCH,2015,37(7):638-649.
APA Huang, Hou-Bin,Zhang, Yi-Xin,Zhang, Shui-Hua,&Ma, Zhi-Zhong.(2015).Calibration and standardisation of graded optic nerve injuries in rats.NEUROLOGICAL RESEARCH,37(7),638-649.
MLA Huang, Hou-Bin,et al."Calibration and standardisation of graded optic nerve injuries in rats".NEUROLOGICAL RESEARCH 37.7(2015):638-649.
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