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学科主题: 临床医学
题名:
Cloning and identification of novel MicroRNAs from rat hippocampus
作者: He, Xiangjun; Zhang, Qi; Liu, Yujing; Pan, Xiuying
关键词: microRNA ; cloning ; hippocampus
刊名: ACTA BIOCHIMICA ET BIOPHYSICA SINICA
发表日期: 2007-09-01
DOI: 10.1111/j.1745-7270.2007.00324.x
卷: 39, 期:9, 页:708-714
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology ; Biophysics
研究领域[WOS]: Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]: DENTATE GYRUS ; NEUROGENESIS ; PCR ; SYSTEM ; GENES ; MOUSE
英文摘要:

MicroRNAs (miRNAs) are small regulatory molecules post-transcriptionally suppressing mRNA activity. Many miRNAs in various organisms have been cloned but many unknown miRNAs remain to be identified. Here we describe the cloning of six new miRNAs from rat hippocampus. Among them, four were not found in the rat miRBase, but were identical to their human and/or mouse homolog, therefore they were designated as rno-miR-92b, rno-miR-146b, rno-let-7g, and rno-miR-551b. The other two were derived from the other arms of the known miRNA precursors of rno-miR-330 and rno-miR-384, and were not found in miRBase of all organisms. They were designated as rno-miR-330* and rno-miR-384*. The expression of these miRNAs was confirmed by RNA-tailing and primer-extension real-time reverse transcription-polymerase chain reaction. These six miRNAs were expressed at significantly higher levels in the hippocampus than in other tissues, including cerebral cortex, heart, liver, lung and kidney. miR-384* was 10 times more abundant than miR-384 in rat hippocampus, but little difference was found between miR-330* and miR-330 expression in the same tissue.

语种: 英语
WOS记录号: WOS:000249515100009
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/67359
Appears in Collections:北京大学第二临床医学院_临床分子生物学研究所_期刊论文

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作者单位: Peking Univ, Peoples Hosp, Inst Clin Mol Biol, Beijing 100044, Peoples R China

Recommended Citation:
He, Xiangjun,Zhang, Qi,Liu, Yujing,et al. Cloning and identification of novel MicroRNAs from rat hippocampus[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2007,39(9):708-714.
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