学科主题临床医学
Cloning and identification of novel MicroRNAs from rat hippocampus
He, Xiangjun; Zhang, Qi; Liu, Yujing; Pan, Xiuying
关键词microRNA cloning hippocampus
刊名ACTA BIOCHIMICA ET BIOPHYSICA SINICA
2007-09-01
DOI10.1111/j.1745-7270.2007.00324.x
39期:9页:708-714
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Biophysics
研究领域[WOS]Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]DENTATE GYRUS ; NEUROGENESIS ; PCR ; SYSTEM ; GENES ; MOUSE
英文摘要

MicroRNAs (miRNAs) are small regulatory molecules post-transcriptionally suppressing mRNA activity. Many miRNAs in various organisms have been cloned but many unknown miRNAs remain to be identified. Here we describe the cloning of six new miRNAs from rat hippocampus. Among them, four were not found in the rat miRBase, but were identical to their human and/or mouse homolog, therefore they were designated as rno-miR-92b, rno-miR-146b, rno-let-7g, and rno-miR-551b. The other two were derived from the other arms of the known miRNA precursors of rno-miR-330 and rno-miR-384, and were not found in miRBase of all organisms. They were designated as rno-miR-330* and rno-miR-384*. The expression of these miRNAs was confirmed by RNA-tailing and primer-extension real-time reverse transcription-polymerase chain reaction. These six miRNAs were expressed at significantly higher levels in the hippocampus than in other tissues, including cerebral cortex, heart, liver, lung and kidney. miR-384* was 10 times more abundant than miR-384 in rat hippocampus, but little difference was found between miR-330* and miR-330 expression in the same tissue.

语种英语
WOS记录号WOS:000249515100009
引用统计
被引频次:23[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/67359
专题北京大学第二临床医学院_临床分子生物学研究所
作者单位Peking Univ, Peoples Hosp, Inst Clin Mol Biol, Beijing 100044, Peoples R China
推荐引用方式
GB/T 7714
He, Xiangjun,Zhang, Qi,Liu, Yujing,et al. Cloning and identification of novel MicroRNAs from rat hippocampus[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2007,39(9):708-714.
APA He, Xiangjun,Zhang, Qi,Liu, Yujing,&Pan, Xiuying.(2007).Cloning and identification of novel MicroRNAs from rat hippocampus.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,39(9),708-714.
MLA He, Xiangjun,et al."Cloning and identification of novel MicroRNAs from rat hippocampus".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 39.9(2007):708-714.
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