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学科主题: 临床医学
题名:
Differentiation of rat bone marrow stem cells in liver after partial hepatectomy
作者: Zhan, Yu-Tao; Wang, Yu; Wei, Lai; Liu, Bin; Chen, Hong-Song; Cong, Xu; Fei, Ran
关键词: bone marrow stem cells ; liver regeneration ; differentiation
刊名: WORLD JOURNAL OF GASTROENTEROLOGY
发表日期: 2006-08-21
卷: 12, 期:31, 页:5051-5054
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Gastroenterology & Hepatology
研究领域[WOS]: Gastroenterology & Hepatology
关键词[WOS]: IN-VIVO ; HEPATOCYTES ; LYMPHOCYTES ; MICE
英文摘要:

AIM: To investigate the differentiation of rat bone marrow stem cells in liver after partial hepatectomy.

METHODS: Bone marrow cells were collected from the tibia of rat with partial hepatectomy, the medial and left hepatic lobes were excised. The bone marrow stem cells (Thy(+)CD3(-)CD45RA(-) cells) were enriched from the bone marrow cells by depleting red cells and fluorescence-activated cell sorting. The sorted bone marrow stem cells were labeled by PKH26-GL in vitro and autotransplanted by portal vein injection. After 2 wk, the transplanted bone marrow stem cells in liver were examined by the immunohistochemistry of albumin (hepatocyte-specific marker).

RESULTS: The bone marrow stem cells (Thy(+)CD3(-)CD45RA(-): cells) accounted for 2.8% of bone marrow cells without red cells. The labeling rate of 10 mu M PKH26-GL on sorted bone marrow stem cells was about 95%. There were sporadic PKH26-GL-labeled cells among hepatocytes in liver tissue section, and some of the cells expressed albumin.

CONCLUSION: Rat bone marrow stem cells can differentiate into hepatocytes in regenerative environment and may participate in liver regeneration after partial hepatectomy. (C) 2006 The WJG Press. All rights reserved.

语种: 英语
WOS记录号: WOS:000240185800019
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/67368
Appears in Collections:北京大学第二临床医学院_北京大学肝病研究所_期刊论文

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作者单位: 1.Capital Med Univ, Beijing Tongren Hosp, Dept Gastroenterol, Beijing 100730, Peoples R China
2.Beijing Univ, Peoples Hosp, Inst Hepatol, Beijing 100044, Peoples R China

Recommended Citation:
Zhan, Yu-Tao,Wang, Yu,Wei, Lai,et al. Differentiation of rat bone marrow stem cells in liver after partial hepatectomy[J]. WORLD JOURNAL OF GASTROENTEROLOGY,2006,12(31):5051-5054.
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