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学科主题: 临床医学
题名:
The possible role of hydrogen sulfide as a smooth muscle cell proliferation inhibitor in rat cultured cells
作者: Du, JB; Hui, Y; Cheung, YF; Bin, G; Jiang, HF; Chen, XB; Tang, CS
关键词: hydrogen sulfide ; endothelin ; aorta ; mitogen-activated protein kinase ; gasotransmitter
刊名: HEART AND VESSELS
发表日期: 2004-03-01
DOI: 10.1007/s00380-003-0743-7
卷: 19, 期:2, 页:75-80
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Cardiac & Cardiovascular Systems ; Peripheral Vascular Disease
研究领域[WOS]: Cardiovascular System & Cardiology
关键词[WOS]: NITRIC-OXIDE ; CARBON-MONOXIDE ; VASORELAXATION ; MECHANISMS ; H2S
英文摘要:

Hydrogen sulfide (H2S) was recently suggested to be a possible endogenous gasotransmitter in physiological concentration. For the purpose of understanding its possible role in the regulation of the cardiovascular system, we explored the potential effect of H2S on the proliferation of cultured aortic vascular smooth muscle cells (VSMCs) of rats and mitrogen-activated protein kinase (MAPK) as a signaling transduction pathway. Vascular smooth muscle cells were cultured in vitro and the cells were divided into six groups: (1) control group, (2) serum group, (3) endothelin group, (4) NaHS group, (5) serum + NaHS group, and (6) endothelin + NaHS group. VSMC proliferation was measured by [H-3]thymidine ([H-3]TdR) incorporation and MAPK activity in the VSMCs was determined by radioactivity assay. The results showed that endothelin-1 increased VSMC [H-3]TdR incorporation 2.39-fold (P<0.01) and MAPK activity 1.62-fold (P<0.01), as compared with controls. Hydrogen sulfide at 5x10(-5) mol/l, 1x10(-4) mol/l, and 5x10(-4) mol/l decreased VSMC [H-3]TdR incorporation by 16.8%, 26.60%, and 37.40%, respectively, and reduced MAPK activity by 7.37% (P>0.05), 23.39%, and 33.57%, respectively (P<0.01). The results demonstrated that H,S could dose-dependently suppress the proliferation of VSMCs through the MAPK pathway.

语种: 英语
WOS记录号: WOS:000220347000004
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/67385
Appears in Collections:北京大学第一临床医学院_儿科_期刊论文

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作者单位: 1.Peking Univ, Hosp 1, Dept Pediat, Beijing 100034, Peoples R China
2.Univ Hong Kong, Grantham Hosp, Dept Pediat Cardiol, Hong Kong, Hong Kong, Peoples R China
3.Peking Univ, Hosp 1, Inst Cardiovasc Dis, Beijing 100871, Peoples R China

Recommended Citation:
Du, JB,Hui, Y,Cheung, YF,et al. The possible role of hydrogen sulfide as a smooth muscle cell proliferation inhibitor in rat cultured cells[J]. HEART AND VESSELS,2004,19(2):75-80.
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