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The possible role of hydrogen sulfide as a smooth muscle cell proliferation inhibitor in rat cultured cells
Du, JB; Hui, Y; Cheung, YF; Bin, G; Jiang, HF; Chen, XB; Tang, CS
关键词hydrogen sulfide endothelin aorta mitogen-activated protein kinase gasotransmitter
刊名HEART AND VESSELS
2004-03-01
DOI10.1007/s00380-003-0743-7
19期:2页:75-80
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Cardiac & Cardiovascular Systems ; Peripheral Vascular Disease
研究领域[WOS]Cardiovascular System & Cardiology
关键词[WOS]NITRIC-OXIDE ; CARBON-MONOXIDE ; VASORELAXATION ; MECHANISMS ; H2S
英文摘要

Hydrogen sulfide (H2S) was recently suggested to be a possible endogenous gasotransmitter in physiological concentration. For the purpose of understanding its possible role in the regulation of the cardiovascular system, we explored the potential effect of H2S on the proliferation of cultured aortic vascular smooth muscle cells (VSMCs) of rats and mitrogen-activated protein kinase (MAPK) as a signaling transduction pathway. Vascular smooth muscle cells were cultured in vitro and the cells were divided into six groups: (1) control group, (2) serum group, (3) endothelin group, (4) NaHS group, (5) serum + NaHS group, and (6) endothelin + NaHS group. VSMC proliferation was measured by [H-3]thymidine ([H-3]TdR) incorporation and MAPK activity in the VSMCs was determined by radioactivity assay. The results showed that endothelin-1 increased VSMC [H-3]TdR incorporation 2.39-fold (P<0.01) and MAPK activity 1.62-fold (P<0.01), as compared with controls. Hydrogen sulfide at 5x10(-5) mol/l, 1x10(-4) mol/l, and 5x10(-4) mol/l decreased VSMC [H-3]TdR incorporation by 16.8%, 26.60%, and 37.40%, respectively, and reduced MAPK activity by 7.37% (P>0.05), 23.39%, and 33.57%, respectively (P<0.01). The results demonstrated that H,S could dose-dependently suppress the proliferation of VSMCs through the MAPK pathway.

语种英语
WOS记录号WOS:000220347000004
引用统计
被引频次:116[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/67385
专题北京大学第一临床医学院_儿科
作者单位1.Peking Univ, Hosp 1, Dept Pediat, Beijing 100034, Peoples R China
2.Univ Hong Kong, Grantham Hosp, Dept Pediat Cardiol, Hong Kong, Hong Kong, Peoples R China
3.Peking Univ, Hosp 1, Inst Cardiovasc Dis, Beijing 100871, Peoples R China
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GB/T 7714
Du, JB,Hui, Y,Cheung, YF,et al. The possible role of hydrogen sulfide as a smooth muscle cell proliferation inhibitor in rat cultured cells[J]. HEART AND VESSELS,2004,19(2):75-80.
APA Du, JB.,Hui, Y.,Cheung, YF.,Bin, G.,Jiang, HF.,...&Tang, CS.(2004).The possible role of hydrogen sulfide as a smooth muscle cell proliferation inhibitor in rat cultured cells.HEART AND VESSELS,19(2),75-80.
MLA Du, JB,et al."The possible role of hydrogen sulfide as a smooth muscle cell proliferation inhibitor in rat cultured cells".HEART AND VESSELS 19.2(2004):75-80.
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