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Polyaspartoyl L-arginine protects endothelial cells against injury
Jian, Yang1; Tang, Zhiyu1; Wang, Yinye1; Peng, Shiqi2
关键词Polyaspartoyl L-arginine Rat aorta endothelial cell Oxidative injury Apoptosis L-arginine Nitric oxide
刊名EUROPEAN JOURNAL OF PHARMACOLOGY
2008-12-03
DOI10.1016/j.ejphar.2008.09.040
599期:1-3页:96-101
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Pharmacology & Pharmacy
研究领域[WOS]Pharmacology & Pharmacy
关键词[WOS]NITRIC-OXIDE ; APOPTOSIS ; PROSTACYCLIN ; STIMULATION ; MACROPHAGES ; THROMBOSIS ; DEATH
英文摘要

Polyaspartoyl L-arginine (PDR) is an anti-thrombotic agent and its anti-thrombotic effect is related with endothelial cells. This study is to investigate the effect of PDR on the endothelial cells. In cell injury assay 1.7-170 mu g/ml of PDR significantly increased the viability of rat aorta endothelial cells (RAECs) injured by H(2)O(2), this effect was comparable with that of 95 mu g/ml of alpha-tocopherol, and was more powerful than that of L-arginine. Nitric oxide synthase(NOS) inhibitor, L-NAME, almost abolished the effect of PDR, but not influence the effect of alpha-tocopherol or L-arginine. PDR enhanced the viability of RAECs injured by oxidized-low density lipoprotein (ox-LDL) either, which was comparable to that of alpha-tocopherol, whereas L-arginine, L-aspartic acid alone or their combined use failed to showed effects. PDR (17-170 mu g/ml) raised nitrite level in RAEC medium, which is the major end-product of NO, but L-arginine (170 mu g/ml) produced insignificant nitrite level rise. In addition, in the absence of RAEC PDR and L-arginine but alpha-tocopherol failed to lower the concentration of oxidative product (Fe(3+)) in a cell free system, whereas in the presence of RAEC PDR L-arginine or alpha-tocopherol all significantly reduced the concentration of Fe(3+). In cell apoptosis assay PDR (17-170 mu g/ml) lowered the percentage of early apoptotic and late apoptotic RAECs, consequently increased the percentage of normal cells. Furthermore PDR significantly inhibited caspase-3 activity in RAECs: this effect is comparable with alpha-tocopherol and more potent than that of L-arginine. In conclusion, PDR is a cell protector. it protects endothelial cell against oxidative injury and apoptosis; its cell protective effect against H(2)O(2) injuries is NOS dependent and is related with NO production; PDR is anti-oxidant, its anti-oxidant effect needs endothelial cell′s participation. The findings suggest PDR may play a much better beneficial role than L-arginine in the prevention and treatment for those diseases with endothelial dysfunction. (C) 2008 Elsevier B.V. All rights reserved.

语种英语
WOS记录号WOS:000261157500015
资助机构National new drug developed funds ; National Economy and Trade Ministry Funds ; North China Pharmaceutical Groups New Drug R D funds
引用统计
被引频次:1[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/67399
专题北京大学药学院
北京大学药学院_分子与细胞药理学系
医学人文研究院/公共教学部_哲学与社会科学系
作者单位1.Peking Univ, Sch Pharmaceut Sci, Dept Mol & Cellular Pharmacol, Beijing 100083, Peoples R China
2.Capital Univ Med Sci, Beijing Key Labs Hydrone & Peptides, Beijing 100069, Peoples R China
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GB/T 7714
Jian, Yang,Tang, Zhiyu,Wang, Yinye,et al. Polyaspartoyl L-arginine protects endothelial cells against injury[J]. EUROPEAN JOURNAL OF PHARMACOLOGY,2008,599(1-3):96-101.
APA Jian, Yang,Tang, Zhiyu,Wang, Yinye,&Peng, Shiqi.(2008).Polyaspartoyl L-arginine protects endothelial cells against injury.EUROPEAN JOURNAL OF PHARMACOLOGY,599(1-3),96-101.
MLA Jian, Yang,et al."Polyaspartoyl L-arginine protects endothelial cells against injury".EUROPEAN JOURNAL OF PHARMACOLOGY 599.1-3(2008):96-101.
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