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学科主题: 临床医学
题名:
Minocycline protects retinal ganglion cells after optic nerve crush injury in mice by delaying autophagy and upregulating nuclear factor-kappa B2
作者: Jiao Xiaoling1,2; Peng Yuan1,2; Yang Liu1,2
关键词: minocycline ; autophagy ; LC3 protein ; nuclear factor-kappa B2 p52 subunit ; retinal ganglion cells ; optic nerve injury
刊名: CHINESE MEDICAL JOURNAL
发表日期: 2014-05-05
DOI: 10.3760/cma.j.issn.0366-6999.20140129
卷: 127, 期:9, 页:1749-1754
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Medicine, General & Internal
研究领域[WOS]: General & Internal Medicine
关键词[WOS]: NF-KAPPA-B ; DEGENERATION ; NEUROPROTECTION ; INHIBITION ; GLAUCOMA ; MOUSE ; REL
英文摘要:

Background Currently, no medicine is available that can prevent or treat neural damage associated with optic nerve injury. Minocycline is recently reported to have a neuroprotective function. The aims of this study were to exarmine the neuroprotective effect of minocycline on retinal ganglion cells (RGCs) and determine its underlying mechanisms, using a mouse model of optic nerve crush (ONC).

Methods ONC was performed in the left eye of adult male mice, and the mice were randomly divided into minocycline-treated group and saline-treated control group. The mice without receiving ONC injury were used as positive controls. RGC densities were assessed in retinal whole mounts with immunofluorescence labeling of beta III-tubulin. Transmission electron microscopy was used to detect RGC morphologies, and Western blotting and real-time PCR were applied to investigate the expression of autophagy markers LC3-I, LC3-II, and transcriptional factors nuclear factor-kappa B1 (NF-kappa B1), NF-kappa B2.

Results In the early stage after ONC (at Days 4 and 7), the density of RGCs in the minocycline-treated group was higher than that of the saline-treated group. Electron micrographs showed that minocycline prevented nuclei and mitochondria injuries at Day 4. Western blotting analysis demonstrated that the conversion of LC3-I to LC3-II was reduced in the minocycline-treated group at Days 4 and 7, which meant autophagy process was inhibited by minocycline. In addition, the gene expression of NF-kappa B2 was upregulated by minocycline at Day 4.

Conclusion The neuroprotective effect of minocycline is generated in the early stage after ONC in mice, partly through delaying autophagy process and regulating NF-kappa B2 pathway.

语种: 英语
所属项目编号: 81170837
项目资助者: National Natural Science Foundation of China
WOS记录号: WOS:000335936800026
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/67412
Appears in Collections:北京大学第一临床医学院_眼科_期刊论文

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作者单位: 1.Peking Univ, Hosp 1, Dept Ophthalmol, Beijing 100034, Peoples R China
2.Minist Educ, Key Lab Vis Loss & Restorat, Beijing 100034, Peoples R China

Recommended Citation:
Jiao Xiaoling,Peng Yuan,Yang Liu. Minocycline protects retinal ganglion cells after optic nerve crush injury in mice by delaying autophagy and upregulating nuclear factor-kappa B2[J]. CHINESE MEDICAL JOURNAL,2014,127(9):1749-1754.
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