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学科主题: 临床医学
题名:
Highly expressed SLC35F2 in non-small cell lung cancer is associated with pathological staging
作者: Bu, Liang; Jiang, Guanchao; Yang, Fan; Liu, Jun; Wang, Jun
关键词: solute carrier family 35 member F2 ; non-small cell lung cancer ; polyclonal antibody ; tissue microarray ; laser capture microdissection
刊名: MOLECULAR MEDICINE REPORTS
发表日期: 2011-11-01
DOI: 10.3892/mmr.2011.572
卷: 4, 期:6, 页:1289-1293
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Oncology ; Medicine, Research & Experimental
研究领域[WOS]: Oncology ; Research & Experimental Medicine
关键词[WOS]: IDENTIFICATION ; CHEMOTHERAPY ; TISSUES ; GENES
英文摘要:

Homo sapiens solute carrier family 35 member F2 (SLC35F2) is highly homologous to the lung squamous cell cancer-related gene, LSCC3, which is highly expressed in lung squamous cell tumour tissues. However, the clinical implication of the SLC35F2 gene in tumour development and progression remains unclear. An affinity-purified polyclonal antibody raised against the human SLC35F2 peptide was used in the immunohistochemical analysis of a non-small cell lung cancer (NSCLC) tissue microarray of human NSCLC (n=129). SLC35F2 protein was also analysed with the same antibody using Western blotting. Total RNAs were extracted from tumour tissues (n=43) and from laser-dissected tumour cells (n=9). SLC35F2 gene expression was detected by fluorescent real-time quantitative PCR and compared to the expression in the corresponding adjacent normal lung tissues. It was found that both the SLC35F2 protein (by IHC analysis) and the SLS35F2 gene transcripts (by Q-PCR analysis) were expressed at significantly higher levels in the NSCLC tumour tissues than in the corresponding adjacent normal lung tissues (p<0.001 and =0.015, respectively). There was a significant correlation between the SLC35F2 transcript and pathological staging (r=0.219, p=0.029), although the correlation between SLC35F2 protein and the staging was not significant (r=0.175). SLC35F2 was highly expressed in NSCLC tissues and the levels of expression, in particular the levels of the SLC35F2 transcript, were associated with NSCLC pathological staging. SLC35F2 appears to have a significant prognostic value in NSCLC.

语种: 英语
所属项目编号: 30772486
项目资助者: National Natural Sciences Foundation of China
WOS记录号: WOS:000295389400040
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/67417
Appears in Collections:北京大学第二临床医学院_胸外科_期刊论文

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作者单位: Peking Univ, Peoples Hosp, Dept Thorac Surg, Beijing 100044, Peoples R China

Recommended Citation:
Bu, Liang,Jiang, Guanchao,Yang, Fan,et al. Highly expressed SLC35F2 in non-small cell lung cancer is associated with pathological staging[J]. MOLECULAR MEDICINE REPORTS,2011,4(6):1289-1293.
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