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学科主题临床医学
Highly expressed SLC35F2 in non-small cell lung cancer is associated with pathological staging
Bu, Liang; Jiang, Guanchao; Yang, Fan; Liu, Jun; Wang, Jun
关键词solute carrier family 35 member F2 non-small cell lung cancer polyclonal antibody tissue microarray laser capture microdissection
刊名MOLECULAR MEDICINE REPORTS
2011-11-01
DOI10.3892/mmr.2011.572
4期:6页:1289-1293
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Oncology ; Medicine, Research & Experimental
资助者National Natural Sciences Foundation of China ; National Natural Sciences Foundation of China
研究领域[WOS]Oncology ; Research & Experimental Medicine
关键词[WOS]IDENTIFICATION ; CHEMOTHERAPY ; TISSUES ; GENES
英文摘要

Homo sapiens solute carrier family 35 member F2 (SLC35F2) is highly homologous to the lung squamous cell cancer-related gene, LSCC3, which is highly expressed in lung squamous cell tumour tissues. However, the clinical implication of the SLC35F2 gene in tumour development and progression remains unclear. An affinity-purified polyclonal antibody raised against the human SLC35F2 peptide was used in the immunohistochemical analysis of a non-small cell lung cancer (NSCLC) tissue microarray of human NSCLC (n=129). SLC35F2 protein was also analysed with the same antibody using Western blotting. Total RNAs were extracted from tumour tissues (n=43) and from laser-dissected tumour cells (n=9). SLC35F2 gene expression was detected by fluorescent real-time quantitative PCR and compared to the expression in the corresponding adjacent normal lung tissues. It was found that both the SLC35F2 protein (by IHC analysis) and the SLS35F2 gene transcripts (by Q-PCR analysis) were expressed at significantly higher levels in the NSCLC tumour tissues than in the corresponding adjacent normal lung tissues (p<0.001 and =0.015, respectively). There was a significant correlation between the SLC35F2 transcript and pathological staging (r=0.219, p=0.029), although the correlation between SLC35F2 protein and the staging was not significant (r=0.175). SLC35F2 was highly expressed in NSCLC tissues and the levels of expression, in particular the levels of the SLC35F2 transcript, were associated with NSCLC pathological staging. SLC35F2 appears to have a significant prognostic value in NSCLC.

语种英语
所属项目编号30772486
资助者National Natural Sciences Foundation of China ; National Natural Sciences Foundation of China
WOS记录号WOS:000295389400040
引用统计
被引频次:5[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/67417
专题北京大学第二临床医学院_胸外科
作者单位Peking Univ, Peoples Hosp, Dept Thorac Surg, Beijing 100044, Peoples R China
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GB/T 7714
Bu, Liang,Jiang, Guanchao,Yang, Fan,et al. Highly expressed SLC35F2 in non-small cell lung cancer is associated with pathological staging[J]. MOLECULAR MEDICINE REPORTS,2011,4(6):1289-1293.
APA Bu, Liang,Jiang, Guanchao,Yang, Fan,Liu, Jun,&Wang, Jun.(2011).Highly expressed SLC35F2 in non-small cell lung cancer is associated with pathological staging.MOLECULAR MEDICINE REPORTS,4(6),1289-1293.
MLA Bu, Liang,et al."Highly expressed SLC35F2 in non-small cell lung cancer is associated with pathological staging".MOLECULAR MEDICINE REPORTS 4.6(2011):1289-1293.
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