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学科主题: 临床医学
题名:
Reversine Enhances Generation of Progenitor-like Cells by Dedifferentiation of Annulus Fibrosus Cells
作者: Saraiya, Mansi1,2; Nasser, Rena1,2; Zeng, Yan1,2,3; Addya, Sankar4; Ponnappan, Ravi Kumar1,2; Fortina, Paolo4; Anderson, David Greg1,2; Albert, Todd J.1,2; Shapiro, Irving M.1,2; Risbud, Makarand V.1,2
刊名: TISSUE ENGINEERING PART A
发表日期: 2010-04-01
DOI: 10.1089/ten.tea.2009.0343
卷: 16, 期:4, 页:1443-1455
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Cell & Tissue Engineering ; Biotechnology & Applied Microbiology ; Cell Biology
研究领域[WOS]: Cell Biology ; Biotechnology & Applied Microbiology
关键词[WOS]: MESENCHYMAL STEM-CELLS ; AURORA-B KINASE ; INTERVERTEBRAL DISC ; IN-VITRO ; DIFFERENTIATION ; MODEL ; TRANSPLANTATION ; THERAPY ; DISEASE
英文摘要:

The aim of this study was to determine if treatment with reversine, a purine analog, promoted generation of skeletal progenitor cells from lineage-committed annulus fibrosus cells. Reversine modulated cell growth, morphology, and the actin cytoskeleton of annulus fibrosus cells. Microarray profiling coupled with Ingenuity Pathway Analysis revealed that reversine treatment resulted in a significant expression change in many genes including those required for cell-cell interaction, cell movement, cell growth, and development. Further analysis revealed that there was involvement of gene networks concerned with cellular assembly and organization, DNA replication and repair, tissue morphology, and cell-to-cell signaling. The gene expression profile was dependent on reversine concentration. In osteogenic media, cells pretreated with 300nM reversine exhibited an increased induction in alkaline phosphatase activity and enhanced expression of alkaline phosphatase, bone sialoprotein, osteocalcin, and collagen type I mRNA. Maintained in adipogenic media, the reversine-pretreated annulus cells displayed evidence of adipogenic differentiation: accumulation of cytosolic lipid droplets and increased expression of PPAR-gamma 2, LPL, and Fabp mRNA. In chondrogenic media, cells pretreated with reversine exhibited marked increase in the induction of aggrecan, collagen types II, IX, and XI, and versican. It is concluded that reversine treatment induced annulus fibrosus cell plasticity and promoted their differentiation along mesenchymal lineages. This agent could be used to generate skeletal progenitor cells to orchestrate the repair of the intervertebral disc.

语种: 英语
所属项目编号: R01AR050087 ; R01AR055655
项目资助者: NIH
WOS记录号: WOS:000276356700030
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/67424
Appears in Collections:北京大学第三临床医学院_骨科_期刊论文

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作者单位: 1.Thomas Jefferson Univ, Dept Orthopaed Surg, Philadelphia, PA 19107 USA
2.Thomas Jefferson Univ, Grad Program Tissue Engn & Regenerat Med, Philadelphia, PA 19107 USA
3.Peking Univ, Dept Orthopaed Surg, Hosp 3, Beijing 100871, Peoples R China
4.Thomas Jefferson Univ, Kimmel Canc Ctr, Dept Canc Biol, Philadelphia, PA 19107 USA

Recommended Citation:
Saraiya, Mansi,Nasser, Rena,Zeng, Yan,et al. Reversine Enhances Generation of Progenitor-like Cells by Dedifferentiation of Annulus Fibrosus Cells[J]. TISSUE ENGINEERING PART A,2010,16(4):1443-1455.
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