IR@PKUHSC  > 北京大学第三临床医学院  > 骨科
学科主题临床医学
Reversine Enhances Generation of Progenitor-like Cells by Dedifferentiation of Annulus Fibrosus Cells
Saraiya, Mansi1,2; Nasser, Rena1,2; Zeng, Yan1,2,3; Addya, Sankar4; Ponnappan, Ravi Kumar1,2; Fortina, Paolo4; Anderson, David Greg1,2; Albert, Todd J.1,2; Shapiro, Irving M.1,2; Risbud, Makarand V.1,2
刊名TISSUE ENGINEERING PART A
2010-04-01
DOI10.1089/ten.tea.2009.0343
16期:4页:1443-1455
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Cell & Tissue Engineering ; Biotechnology & Applied Microbiology ; Cell Biology
研究领域[WOS]Cell Biology ; Biotechnology & Applied Microbiology
关键词[WOS]MESENCHYMAL STEM-CELLS ; AURORA-B KINASE ; INTERVERTEBRAL DISC ; IN-VITRO ; DIFFERENTIATION ; MODEL ; TRANSPLANTATION ; THERAPY ; DISEASE
英文摘要

The aim of this study was to determine if treatment with reversine, a purine analog, promoted generation of skeletal progenitor cells from lineage-committed annulus fibrosus cells. Reversine modulated cell growth, morphology, and the actin cytoskeleton of annulus fibrosus cells. Microarray profiling coupled with Ingenuity Pathway Analysis revealed that reversine treatment resulted in a significant expression change in many genes including those required for cell-cell interaction, cell movement, cell growth, and development. Further analysis revealed that there was involvement of gene networks concerned with cellular assembly and organization, DNA replication and repair, tissue morphology, and cell-to-cell signaling. The gene expression profile was dependent on reversine concentration. In osteogenic media, cells pretreated with 300nM reversine exhibited an increased induction in alkaline phosphatase activity and enhanced expression of alkaline phosphatase, bone sialoprotein, osteocalcin, and collagen type I mRNA. Maintained in adipogenic media, the reversine-pretreated annulus cells displayed evidence of adipogenic differentiation: accumulation of cytosolic lipid droplets and increased expression of PPAR-gamma 2, LPL, and Fabp mRNA. In chondrogenic media, cells pretreated with reversine exhibited marked increase in the induction of aggrecan, collagen types II, IX, and XI, and versican. It is concluded that reversine treatment induced annulus fibrosus cell plasticity and promoted their differentiation along mesenchymal lineages. This agent could be used to generate skeletal progenitor cells to orchestrate the repair of the intervertebral disc.

语种英语
WOS记录号WOS:000276356700030
引用统计
被引频次:24[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/67424
专题北京大学第三临床医学院_骨科
作者单位1.Thomas Jefferson Univ, Dept Orthopaed Surg, Philadelphia, PA 19107 USA
2.Thomas Jefferson Univ, Grad Program Tissue Engn & Regenerat Med, Philadelphia, PA 19107 USA
3.Peking Univ, Dept Orthopaed Surg, Hosp 3, Beijing 100871, Peoples R China
4.Thomas Jefferson Univ, Kimmel Canc Ctr, Dept Canc Biol, Philadelphia, PA 19107 USA
推荐引用方式
GB/T 7714
Saraiya, Mansi,Nasser, Rena,Zeng, Yan,et al. Reversine Enhances Generation of Progenitor-like Cells by Dedifferentiation of Annulus Fibrosus Cells[J]. TISSUE ENGINEERING PART A,2010,16(4):1443-1455.
APA Saraiya, Mansi.,Nasser, Rena.,Zeng, Yan.,Addya, Sankar.,Ponnappan, Ravi Kumar.,...&Risbud, Makarand V..(2010).Reversine Enhances Generation of Progenitor-like Cells by Dedifferentiation of Annulus Fibrosus Cells.TISSUE ENGINEERING PART A,16(4),1443-1455.
MLA Saraiya, Mansi,et al."Reversine Enhances Generation of Progenitor-like Cells by Dedifferentiation of Annulus Fibrosus Cells".TISSUE ENGINEERING PART A 16.4(2010):1443-1455.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Saraiya, Mansi]的文章
[Nasser, Rena]的文章
[Zeng, Yan]的文章
百度学术
百度学术中相似的文章
[Saraiya, Mansi]的文章
[Nasser, Rena]的文章
[Zeng, Yan]的文章
必应学术
必应学术中相似的文章
[Saraiya, Mansi]的文章
[Nasser, Rena]的文章
[Zeng, Yan]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。