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Involvement of follicular basement membrane and vascular endothelium in blood-follicle barrier formation of mice revealed by ′in vivo cryotechnique′
Zhou, Hong; Ohno, Nobuhiko; Terada, Nobuo; Saitoh, Sei; Fujii, Yasuhisa; Ohno, Shinichi
刊名REPRODUCTION
2007-08-01
DOI10.1530/REP-07-0062
134期:2页:307-317
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Developmental Biology ; Reproductive Biology
研究领域[WOS]Developmental Biology ; Reproductive Biology
关键词[WOS]FREEZE-SUBSTITUTION ; TIGHT JUNCTIONS ; LIVING MOUSE ; BRAIN-BARRIER ; IMMUNOHISTOCHEMICAL ANALYSES ; EXTRACELLULAR-MATRIX ; CHARGE SELECTIVITY ; OVARIAN-FOLLICLES ; ANIMAL ORGANS ; PERMEABILITY
英文摘要

The molecular sieve with size- and charge selectivity in ovarian follicles, the so-called blood-follicle barrier (BFB), was examined during follicular development under physiological conditions to reveal ovarian structures responsible for the BFB by using our ′in vivo cryotechnique′ (IVCT). Mouse ovary specimens were prepared with different methods including lVCT, immersion, or perfusion chemical fixation and quick-freezing following resection or perfusion. Their paraffin sections or cryosections were stained with hematoxylin-eosin or immunostained for serum proteins with different molecular weights: albumin, immunoglobulin (Ig) G1 heavy chain, inter-alpha-trypsin inhibitor (I alpha I), fibrinogen, and IgM. Their immunoreactivity was better preserved with IVCT. The immunostaining for albumin was clearly observed in blood vessels, interstitium, and developing follicles, but that of IgGI, I alpha I, or fibrinogen was significantly decreased inside the follicles. IgM was immunohistochernically decreased throughout the interstitium outside blood vessels. The immunoreactivities of IgG1 and IgM, as compared with albumin, were clearly changed along follicular basement membranes and around vascular endothelial cells respectively. These findings indicate that BFB functions throughout follicular development, and the follicular basement membrane and the vascular endothelium could play some significant roles in the permselectivity for such soluble proteins with intermediate and high molecular weight respectively.

语种英语
WOS记录号WOS:000248806900011
Citation statistics
Cited Times:21[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/67431
Collection北京大学公共卫生学院
作者单位1.Yamanashi Univ, Interdisciplinary Grad Sch Med & Engn, Dept Anat, Yamanashi, Japan
2.Peking Univ, Sch Publ Hlth, Adolescent & Womens Hlth, Dept Child, Beijing 100083, Peoples R China
Recommended Citation
GB/T 7714
Zhou, Hong,Ohno, Nobuhiko,Terada, Nobuo,et al. Involvement of follicular basement membrane and vascular endothelium in blood-follicle barrier formation of mice revealed by ′in vivo cryotechnique′[J]. REPRODUCTION,2007,134(2):307-317.
APA Zhou, Hong,Ohno, Nobuhiko,Terada, Nobuo,Saitoh, Sei,Fujii, Yasuhisa,&Ohno, Shinichi.(2007).Involvement of follicular basement membrane and vascular endothelium in blood-follicle barrier formation of mice revealed by ′in vivo cryotechnique′.REPRODUCTION,134(2),307-317.
MLA Zhou, Hong,et al."Involvement of follicular basement membrane and vascular endothelium in blood-follicle barrier formation of mice revealed by ′in vivo cryotechnique′".REPRODUCTION 134.2(2007):307-317.
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