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学科主题: 公共卫生
题名:
Involvement of follicular basement membrane and vascular endothelium in blood-follicle barrier formation of mice revealed by ′in vivo cryotechnique′
作者: Zhou, Hong; Ohno, Nobuhiko; Terada, Nobuo; Saitoh, Sei; Fujii, Yasuhisa; Ohno, Shinichi
刊名: REPRODUCTION
发表日期: 2007-08-01
DOI: 10.1530/REP-07-0062
卷: 134, 期:2, 页:307-317
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Developmental Biology ; Reproductive Biology
研究领域[WOS]: Developmental Biology ; Reproductive Biology
关键词[WOS]: FREEZE-SUBSTITUTION ; TIGHT JUNCTIONS ; LIVING MOUSE ; BRAIN-BARRIER ; IMMUNOHISTOCHEMICAL ANALYSES ; EXTRACELLULAR-MATRIX ; CHARGE SELECTIVITY ; OVARIAN-FOLLICLES ; ANIMAL ORGANS ; PERMEABILITY
英文摘要:

The molecular sieve with size- and charge selectivity in ovarian follicles, the so-called blood-follicle barrier (BFB), was examined during follicular development under physiological conditions to reveal ovarian structures responsible for the BFB by using our ′in vivo cryotechnique′ (IVCT). Mouse ovary specimens were prepared with different methods including lVCT, immersion, or perfusion chemical fixation and quick-freezing following resection or perfusion. Their paraffin sections or cryosections were stained with hematoxylin-eosin or immunostained for serum proteins with different molecular weights: albumin, immunoglobulin (Ig) G1 heavy chain, inter-alpha-trypsin inhibitor (I alpha I), fibrinogen, and IgM. Their immunoreactivity was better preserved with IVCT. The immunostaining for albumin was clearly observed in blood vessels, interstitium, and developing follicles, but that of IgGI, I alpha I, or fibrinogen was significantly decreased inside the follicles. IgM was immunohistochernically decreased throughout the interstitium outside blood vessels. The immunoreactivities of IgG1 and IgM, as compared with albumin, were clearly changed along follicular basement membranes and around vascular endothelial cells respectively. These findings indicate that BFB functions throughout follicular development, and the follicular basement membrane and the vascular endothelium could play some significant roles in the permselectivity for such soluble proteins with intermediate and high molecular weight respectively.

语种: 英语
WOS记录号: WOS:000248806900011
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/67431
Appears in Collections:北京大学公共卫生学院_期刊论文

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作者单位: 1.Yamanashi Univ, Interdisciplinary Grad Sch Med & Engn, Dept Anat, Yamanashi, Japan
2.Peking Univ, Sch Publ Hlth, Adolescent & Womens Hlth, Dept Child, Beijing 100083, Peoples R China

Recommended Citation:
Zhou, Hong,Ohno, Nobuhiko,Terada, Nobuo,et al. Involvement of follicular basement membrane and vascular endothelium in blood-follicle barrier formation of mice revealed by ′in vivo cryotechnique′[J]. REPRODUCTION,2007,134(2):307-317.
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