IR@PKUHSC  > 北京大学基础医学院
学科主题基础医学
Homocysteine upregulates resistin production from adipocytes in vivo and in vitro
Li, Yin; Jiang, Changtao; Xu, Guoheng; Wang, Nanping; Zhu, Yi; Tang, Chaoshu; Wang, Xian1
刊名DIABETES
2008-04-01
57期:4页:817-827
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Endocrinology & Metabolism
研究领域[WOS]Endocrinology & Metabolism
关键词[WOS]MONOCYTE CHEMOATTRACTANT PROTEIN-1 ; TYPE-2 DIABETES-MELLITUS ; FACTOR-KAPPA-B ; INSULIN-RESISTANCE ; 3T3-L1 ADIPOCYTES ; OXIDATIVE STRESS ; GLUCOSE-UPTAKE ; KINASE-C ; ENDOTHELIAL-CELLS ; VASCULAR-DISEASE
英文摘要

OBJECTIVE - Homocysteine (Hcy) is epidemiologically related to insulin resistance, which has been speculated to be a low-grade systemic inflammatory condition. Resistin acts as a critical mediator of insulin resistance associated with inflammatory conditions. We aimed to determine whether Hey can induce insulin resistance by directly regulating the expression and secretion of resistin from adipose tissue.

RESEARCH DESIGN AND METHODS - The effect of Hey on the expression and secretion of resistin and insulin resistance was investigated using primary rat adipocytes and mice with hyperhomocysteinemia (HHcy).

RESULTS - Hcy impaired glucose transport and, particularly, the insulin signaling pathway as shown by decreased insulin-stimulated tyrosine phosphorylation of insulin receptor and insulin receptor substrate (IRS)-1, increased serine phosphorylation of IRS-1, and inhibited Akt phosphorylation both in vitro and in vivo, and these impairments were accompanied by an increase in resistin expression. Compared with normal mice, HHcy mice with a clinically relevant level of plasma Hey (19 mu mol/l) showed significantly increased resistin production from adipose tissue (33.38 +/- 3.08 vs. 19.27 +/- 1.71 ng/ml, P < 0.01). Hey (300-1000 mu mot/l) also increased mRNA expression of resistin in primary rat adipocytes in a time- and concentration-dependent manner, with maximal induction at 24 h of approximately fourfold with 1,000 mu mol/l. In addition, Hcy-induced resistin expression attenuated by treatment with reactive oxygen species (ROS) scavengers, protein kinase C (PKC), and nuclear factor (NF)-kappa B inhibitors implies a role in the process for ROS, PKC, and NF-kappa B.

CONCLUSIONS - HHcy may promote insulin resistance through the induction of resistin expression and secretion from adipocytes via the activation of the ROS-PKC-NF-kappa B pathway.

语种英语
WOS记录号WOS:000254591700005
引用统计
被引频次:54[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/67440
专题北京大学基础医学院
作者单位1.Minist Educ, Key Lab Mol Cardiovasc Sci, Beijing, Peoples R China
2.Peking Univ, Sch Basic Med Sci, Dept Physiol & Pathophysiol, Beijing 100083, Peoples R China
推荐引用方式
GB/T 7714
Li, Yin,Jiang, Changtao,Xu, Guoheng,et al. Homocysteine upregulates resistin production from adipocytes in vivo and in vitro[J]. DIABETES,2008,57(4):817-827.
APA Li, Yin.,Jiang, Changtao.,Xu, Guoheng.,Wang, Nanping.,Zhu, Yi.,...&Wang, Xian.(2008).Homocysteine upregulates resistin production from adipocytes in vivo and in vitro.DIABETES,57(4),817-827.
MLA Li, Yin,et al."Homocysteine upregulates resistin production from adipocytes in vivo and in vitro".DIABETES 57.4(2008):817-827.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Li, Yin]的文章
[Jiang, Changtao]的文章
[Xu, Guoheng]的文章
百度学术
百度学术中相似的文章
[Li, Yin]的文章
[Jiang, Changtao]的文章
[Xu, Guoheng]的文章
必应学术
必应学术中相似的文章
[Li, Yin]的文章
[Jiang, Changtao]的文章
[Xu, Guoheng]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。