北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 北京大学第三临床医学院  > 运动医学研究所  > 期刊论文
学科主题: 临床医学
题名:
Effects of transforming growth factor-beta 1 and vascular endothelial growth factor 165 gene transfer on Achilles tendon healing
作者: Hou, Yu1; Mao, ZeBin2; Wei, XueLei1; Lin, Lin1; Chen, LianXu1; Wang, HaiJun1; Fu, Xin1; Zhang, JiYing1; Yu, Changlong1
关键词: TGF-beta 1 ; VEGF(165) ; Gene therapy ; Adenovirus ; BMSC ; Achilles tendon
刊名: MATRIX BIOLOGY
发表日期: 2009-07-01
DOI: 10.1016/j.matbio.2009.04.007
卷: 28, 期:6, 页:324-335
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology ; Cell Biology
研究领域[WOS]: Biochemistry & Molecular Biology ; Cell Biology
关键词[WOS]: MESENCHYMAL STEM-CELLS ; ANTERIOR CRUCIATE LIGAMENT ; MEDIAL COLLATERAL LIGAMENT ; DIGITAL FLEXOR TENDON ; FACTOR-BETA ; IN-VITRO ; TGF-BETA ; PATELLAR TENDON ; MECHANICAL-PROPERTIES ; SIGNALING PATHWAY
英文摘要:

Repaired Achilles tendons typically take weeks before they are strong enough to handle physiological loads. Gene therapy is a promising treatment for Achilles tendon defects. The aim of the present study was to evaluate the histological/biomechanical effects of Transforming growth factor-beta 1 (TGF-beta 1) and vascular endothelial growth factor 165 (VEGF(165)) gene transfer on Achilles tendon healing in rabbits. Bone Marrow-Derived Mesenchymal Stem Cells (BMSCs) were transduced with adenovirus carrying human TGF-beta 1 cDNA (Ad-TGF-beta 1), human VEGF(165) cDNA (Ad-VEGF(165)), or both (PIRES-TGF-beta 1/VEGF(165)) Viruses, no cDNA (Ad-GFP), and the BMSCs without gene transfer and the intact tendon were used as control. BMSCs were surgically implanted into the experimentally injured Achilles tendons. TGF-beta 1 distribution, cellularity, nuclear aspect ratio, nuclear orientation angle, vascular number, collagen synthesis, and biomechanical features were measured at 1, 2, 4, and 8 weeks after surgery. The TGF-beta 1 and TGF-beta 1/VEGF(165) co-expression groups exhibited improved parameters compared with other groups, while the VEGF(165) expression group had a negative impact. In the co-expression group, the angiogenesis effects of VEGF(165) were diminished by TGF-beta 1, while the collagen synthesis effects of TGF-beta 1 were unaltered by VEGF(165). Thus treatment with TGF-beta 1 cDNA-transduced BMSCs grafts is a promising therapy for acceleration and improvement of tendon healing, leading to quicker recovery and improved biomechanical properties of Achilles tendons. (C) 2009 Elsevier B.V. All rights reserved.

语种: 英语
所属项目编号: 985-2-028-24
项目资助者: Ministry of Education of the People&prime ; s Republic of China
WOS记录号: WOS:000270770100004
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/67464
Appears in Collections:北京大学第三临床医学院_运动医学研究所_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Peking Univ, Beijing, Peoples R China
2.Peking Univ Third Hosp, Inst Sports Med, Beijing, Peoples R China

Recommended Citation:
Hou, Yu,Mao, ZeBin,Wei, XueLei,et al. Effects of transforming growth factor-beta 1 and vascular endothelial growth factor 165 gene transfer on Achilles tendon healing[J]. MATRIX BIOLOGY,2009,28(6):324-335.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Hou, Yu]'s Articles
[Mao, ZeBin]'s Articles
[Wei, XueLei]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Hou, Yu]‘s Articles
[Mao, ZeBin]‘s Articles
[Wei, XueLei]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace