|Inhibition of the cystathionine-gamma-lyase/hydrogen sulfide pathway in rat vascular smooth muscle cells by cobalt-60 gamma radiation|
|Zhong Guang-zhen2; Yang Xin-chun2; Jia Li-ping3; Chen Feng-rong1; Cui Ming1|
|关键词||radiation restenosis hydrogen sulfide vascular smooth muscle cells|
|刊名||CHINESE MEDICAL JOURNAL|
|WOS标题词||Science & Technology|
|类目[WOS]||Medicine, General & Internal|
|研究领域[WOS]||General & Internal Medicine|
|关键词[WOS]||HYDROGEN-SULFIDE ; NITRIC-OXIDE ; BRACHYTHERAPY ; HYPERTENSION ; RESTENOSIS ; GENERATION ; GENE ; H2S|
Background Radiation is a promising treatment for in stent restenosis and restenosis following percutaneous transluminal coronary angioplasty, which has troubled interventional cardiologists for a long time. It inhibits neointima hyperplasia, vascular remodeling, and increases the mean luminal diameter. The mechanism of intracoronary brachytherapy for restenosis is not well understood. Endogenous gaseous transmitters including nitric oxide and carbon monoxide are closely related to restenosis. Hydrogen sulfide, a new endogenous gaseous transmitter, is able to inhibit the proliferation of vascular smooth muscle cells and vascular remodeling. This study aimed to clarify the effect of radiation on cystathionine-gamma-lyase/hydrogen sulfide pathway in rat smooth muscle cells.
Methods We studied the effect of radiation on the cystathionine-gamma-lyase/hydrogen sulfide pathway. Rat vascular smooth muscle cells were radiated with (60)Co gamma at doses of 14 Gy and 25 Gy respectively. Then the mRNA level of cystathionine-gamma-lyase was studied by quantitative reverse-transcription competitive polymerase chain reaction. Hydrogen sulfide concentration in culture medium was determined by methylene blue spectrophotometry. Cystathionine-gamma-lyase activity in vascular smooth muscle cells was also studied.
Results (60)Co gamma radiation at a dose of 1 Gy did not affect the cystathionine-gamma-lyase/hydrogen sulfide pathway significantly. However, (60)Co gamma radiation at doses of 14 Gy and 25 Gy decreased the hydrogen sulfide synthesis by 21.9% (P<0.05) and 26.8% (P<0.01) respectively. At the same time, they decreased the cystathionine-gamma-lyase activity by 15.1% (P<0.05) and 20.5% (P<0.01) respectively, and cystathionine-gamma-lyase mRNA expression by 29.3% (P<0.01) and 38.2% (P<0.01) respectively.
Conclusion Appropriate (60)Co gamma radiation inhibits the H(2)S synthesis by inhibiting the gene expression of cystathionine-gamma-lyase and the cystathionine-gamma-lyase activity.
|项目编号||39970804 ; 30070824 ; 7072027|
|资助机构||National Natural Science Foundation of China ; Beijing Natural Science Foundation|
|作者单位||1.Peking Univ, Hosp 3, Dept Cardiol, Beijing 100083, Peoples R China|
2.Capital Med Univ, Beijing Chaoyang Hosp, Dept Cardiol, Beijing 100020, Peoples R China
3.Chongqing Ninth People Hosp, Dept Gastroenterol, Chongqing 400700, Peoples R China
|Zhong Guang-zhen,Yang Xin-chun,Jia Li-ping,et al. Inhibition of the cystathionine-gamma-lyase/hydrogen sulfide pathway in rat vascular smooth muscle cells by cobalt-60 gamma radiation[J]. CHINESE MEDICAL JOURNAL,2009,122(3):326-330.|
|APA||Zhong Guang-zhen,Yang Xin-chun,Jia Li-ping,Chen Feng-rong,&Cui Ming.(2009).Inhibition of the cystathionine-gamma-lyase/hydrogen sulfide pathway in rat vascular smooth muscle cells by cobalt-60 gamma radiation.CHINESE MEDICAL JOURNAL,122(3),326-330.|
|MLA||Zhong Guang-zhen,et al."Inhibition of the cystathionine-gamma-lyase/hydrogen sulfide pathway in rat vascular smooth muscle cells by cobalt-60 gamma radiation".CHINESE MEDICAL JOURNAL 122.3(2009):326-330.|