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学科主题基础医学
Cytotoxicity of a novel fibroblast growth factor receptor targeted immunotoxin on a human ovarian teratocarcinoma cell line
Zhang, Huajie; Zhang, Shumin; Zhuang, Hui; Lu, Fengmin
关键词diphtheria toxin basic fibroblast growth factor fibroblast growth factor receptor immunotoxin immunofluorescence
刊名CANCER BIOTHERAPY AND RADIOPHARMACEUTICALS
2006-08-01
21期:4页:321-332
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Oncology ; Medicine, Research & Experimental ; Pharmacology & Pharmacy ; Radiology, Nuclear Medicine & Medical Imaging
研究领域[WOS]Oncology ; Research & Experimental Medicine ; Pharmacology & Pharmacy ; Radiology, Nuclear Medicine & Medical Imaging
关键词[WOS]DIPHTHERIA-TOXIN ; INTERLEUKIN-3 RECEPTOR ; FUSION PROTEINS ; CANCER ; PROLIFERATION ; ANGIOGENESIS ; INHIBITION ; MECHANISMS ; LEUKEMIA
英文摘要

Basic fibroblast growth factor (bFGF) is an important tumor-associated growth factor that contributes to proliferation and angiogenesis of tumor. The high-affinity receptor for bFGF, fibroblast growth factor receptor (FGFR), is found to be overexpressed in a number of tumor cells. For the purpose of exploring the significance of bFGF/FGFR in tumor-targeted therapy, a recombinant immunotoxin contained the N-terminal 389 residues of diphtheria toxin (DT), and the full length of human bFGF was designed, expressed, and purified. The bioactivity of the product was evaluated by testing the cytotoxicity on PA-1 cells (a human ovarian teratocarcinoma cell line with high-level expression of FGFR) in vitro. The immunotoxin showed a significant cytotoxicity on PA-1 cells (IC50 8 similar to 9 ng/mL), and this effect could be antagonized by equine diphtheria antitoxin (DAT), bFGF, anti-bFGF monoclonal antibody (MAb), and anti-FGFR polyclonal antibody (PAb), respectively. Additionally, Chinese hamster ovary (CHO) cells and Hep-2 cells (a human epidermoid laryngocarcinoma cell line) with low expression of FGFR were tested to be resistant to the immunotoxin. The results indicated that FGFR might be an effective target for tumor therapy, and bFGF-mediated immunotoxin could be a potential candidate in the treatment of cancer.

语种英语
WOS记录号WOS:000240899900004
引用统计
被引频次:11[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/67470
专题基础医学院_病原生物学系
作者单位1.Normal Inst Control Pharmaceut & Biol Prod, Serum Div, Beijing 100050, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Dept Microbiol, Beijing 100871, Peoples R China
推荐引用方式
GB/T 7714
Zhang, Huajie,Zhang, Shumin,Zhuang, Hui,et al. Cytotoxicity of a novel fibroblast growth factor receptor targeted immunotoxin on a human ovarian teratocarcinoma cell line[J]. CANCER BIOTHERAPY AND RADIOPHARMACEUTICALS,2006,21(4):321-332.
APA Zhang, Huajie,Zhang, Shumin,Zhuang, Hui,&Lu, Fengmin.(2006).Cytotoxicity of a novel fibroblast growth factor receptor targeted immunotoxin on a human ovarian teratocarcinoma cell line.CANCER BIOTHERAPY AND RADIOPHARMACEUTICALS,21(4),321-332.
MLA Zhang, Huajie,et al."Cytotoxicity of a novel fibroblast growth factor receptor targeted immunotoxin on a human ovarian teratocarcinoma cell line".CANCER BIOTHERAPY AND RADIOPHARMACEUTICALS 21.4(2006):321-332.
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