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学科主题临床医学
Recombinant adenovirus-p53 (Gendicine) sensitizes a pancreatic carcinoma cell line to radiation
Li, Jinluan1; Pan, Jianji1; Zhu, Xianggao2; Su, Ying1; Bao, Lingling1; Qiu, Sufang1; Zou, Changyan1; Cai, Yong3; Wu, Junxin1; Tham, Ivan W. K.4
关键词Pancreatic carcinoma recombinant adenovirus-p53 (rAd-p53) transfection radiosensitization
刊名CHINESE JOURNAL OF CANCER RESEARCH
2013-12-01
DOI10.3978/j.issn.1000-9604.2013.11.12
25期:6页:715-721
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Oncology
研究领域[WOS]Oncology
关键词[WOS]P53 GENE-TRANSFER ; ADENOCARCINOMA CELLS ; TUMOR-REGRESSION ; CANCER ; RADIOTHERAPY ; THERAPY ; DELIVERY ; THERAPEUTICS ; GEMCITABINE ; COMBINATION
英文摘要

Objective: In this study, we examine the effects of recombinant adenovirus-p53 (rAd-p53) on the pancreatic carcinoma cell line SW1990. Specifically, we determine if expression of rAd-p53 sensitizes these cells to radiation.

Methods: Following transfection of SW1990 cells with rAd-p53, we measured expression of P53, P21 and Bax by immunocytochemistry. Both transfected and control cell lines were irradiated with a range of doses, and the survival fractions (SF) were calculated. Dose survival curves were constructed and modeled for comparison.

Results: Transfection of SW1990 cells with rAd-p53 resulted in increased expression of P53, P21 and Box in a time-dependent manner. At 96 h after transfection, 89.92% of cells expressed P53, 56.8% expressed P21, and 76.50% expressed Bax. The SF following radiation was lower in the rAd-p53 transfected cells compared to the control cells, suggesting that rAd-p53 sensitizes SW1990 cells to radiation (D-0 for the experimental and control groups was 2.199 and 2.462, respectively).

Conclusions: Use of the adenoviral vector is an effective means of transfecting SW1990 cells with wild-type P53, and this sensitizes the cell line to irradiation. This work suggests that combining rAd-p53 with radiation therapy in pancreatic cancer may be therapeutically beneficial.

语种英语
WOS记录号WOS:000329477800012
项目编号2012J05139 ; Z111107058811021
资助机构Fujian Province Natural Science Foundation ; Beijing Municipal Science &amp ; Technology Commission
引用统计
被引频次:7[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/67471
专题北京大学第二临床医学院_放疗科
北京大学临床肿瘤学院_肿瘤放疗科
作者单位1.Fujian Med Univ, Teaching Hosp, Fujian Prov Canc Hosp, Dept Radiat Oncol, Fuzhou 350014, Peoples R China
2.Peking Univ, Peoples Hosp, Dept Radiat Oncol, Beijing 100044, Peoples R China
3.Peking Univ, Canc Hosp & Inst, Dept Radiat Oncol, Key Lab Carcinogenesis & Translat Res,Minist Educ, Beijing 100142, Peoples R China
4.Natl Univ Singapore, Inst Canc, Dept Radiat Oncol, Singapore 119228, Singapore
推荐引用方式
GB/T 7714
Li, Jinluan,Pan, Jianji,Zhu, Xianggao,et al. Recombinant adenovirus-p53 (Gendicine) sensitizes a pancreatic carcinoma cell line to radiation[J]. CHINESE JOURNAL OF CANCER RESEARCH,2013,25(6):715-721.
APA Li, Jinluan.,Pan, Jianji.,Zhu, Xianggao.,Su, Ying.,Bao, Lingling.,...&Tham, Ivan W. K..(2013).Recombinant adenovirus-p53 (Gendicine) sensitizes a pancreatic carcinoma cell line to radiation.CHINESE JOURNAL OF CANCER RESEARCH,25(6),715-721.
MLA Li, Jinluan,et al."Recombinant adenovirus-p53 (Gendicine) sensitizes a pancreatic carcinoma cell line to radiation".CHINESE JOURNAL OF CANCER RESEARCH 25.6(2013):715-721.
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