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Response and Adaptation of Escherichia coli to Suppression of the Amber Stop Codon
Wang, Qian1,2; Sun, Tingting1,3; Xu, Jianfeng1; Shen, Zhouxin4; Briggs, Steven P.4; Zhou, Demin3; Wang, Lei1
关键词amber codon suppression amino acids expansion of the genetic code mutagenesis synthetic biology
刊名CHEMBIOCHEM
2014-08-18
DOI10.1002/cbic.201402235
15期:12页:1744-1749
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Chemistry, Medicinal
研究领域[WOS]Biochemistry & Molecular Biology ; Pharmacology & Pharmacy
关键词[WOS]UNNATURAL AMINO-ACIDS ; SITE-SPECIFIC INCORPORATION ; GENETIC-CODE ; MAMMALIAN-CELLS ; TRANSFER-RNAS ; PROTEINS ; TRANSPOSITION ; NONSENSE ; SYSTEM
英文摘要

Some extant organisms reassign the amber stop codon to a sense codon through evolution, and suppression of the amber codon with engineered tRNAs has been exploited to expand the genetic code for incorporating non-canonical amino acids (ncAAs) in live systems. However, it is unclear how the host cells respond and adapt to such amber suppression. Herein we suppressed the amber codon in Escherichia coli with an orthogonal tRNA/synthetase pair and cultured the cells under such a pressure for about 500 generations. We discovered that E. coli quickly counteracted the suppression with transposon insertion to inactivate the orthogonal synthetase. Persistent amber suppression evading transposon inactivation led to global proteomic changes with a notable up-regulation of a previously uncharacterized protein (YdiI) for which we identified an unexpected function of expelling plasmids. These results should be valuable for understanding codon reassignment in genetic code evolution and for improving the efficiency of ncAA incorporation.

语种英语
WOS记录号WOS:000340506200007
项目编号1DP20D004744-01 ; P30A014195
资助机构US National Institutes of Health
引用统计
被引频次:7[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/67481
专题北京大学药学院
北京大学药学院_药学院
北京大学临床肿瘤学院_医院感染管理与疾病预防控制科
作者单位1.Salk Inst Biol Studies, Jack H Skirball Ctr Chem Biol & Prote, La Jolla, CA 92037 USA
2.China Agr Univ, Coll Vet Med, Beijing 100193, Peoples R China
3.Peking Univ, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100191, Peoples R China
4.Univ Calif San Diego, Sect Cell & Dev Biol, La Jolla, CA 92093 USA
推荐引用方式
GB/T 7714
Wang, Qian,Sun, Tingting,Xu, Jianfeng,et al. Response and Adaptation of Escherichia coli to Suppression of the Amber Stop Codon[J]. CHEMBIOCHEM,2014,15(12):1744-1749.
APA Wang, Qian.,Sun, Tingting.,Xu, Jianfeng.,Shen, Zhouxin.,Briggs, Steven P..,...&Wang, Lei.(2014).Response and Adaptation of Escherichia coli to Suppression of the Amber Stop Codon.CHEMBIOCHEM,15(12),1744-1749.
MLA Wang, Qian,et al."Response and Adaptation of Escherichia coli to Suppression of the Amber Stop Codon".CHEMBIOCHEM 15.12(2014):1744-1749.
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