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学科主题: 临床医学
题名:
M-ds-P21 induces cell apoptosis in bladder cancer T24 cells through P53 independent pathway
作者: Wang, Haifeng; Liu, Wujiang1,2; Jin, Jie; Zhou, Liqun; Liang, Lili; Guo, Yinglu
关键词: 2 &prime ; -Fluoride modification of ds-P21 ; apoptosis ; bladder cancer cells ; ds-P21 ; T24
刊名: JOURNAL OF CANCER RESEARCH AND THERAPEUTICS
发表日期: 2013
DOI: 10.4103/0973-1482.110367
卷: 9, 期:1, 页:54-59
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Oncology
研究领域[WOS]: Oncology
关键词[WOS]: RNA ; COMPLEX ; PHOSPHORYLATION ; ACTIVATION ; DUPLEXES ; SIRNAS ; DEATH ; P21
英文摘要:

Objectives: To investigate the effect of M-ds-P21 on the apoptosis of bladder cancer T24 cells and its potential mechanism.

Materials and Methods: Effect of M-ds-P21 on T24 cells were assessed by cell morphology and Western blot. Apoptosis was quantified by Annexin-V flow-cytometry analysis. To uncover the role of P53 in M-ds-P21-mediated apoptosis of T24 cells, we knocked down P53 before treating cells with M-ds-P21, and then assayed P21 and apoptosis-related protein by Western blot. To uncover the mechanism by which M-ds-P21 played stronger effect than ds-P21, we performed confocal microscope analyses.

Results: Both M-ds-P21 and ds-P21 treatment changed the cell morphology, leading to cell apoptosis after 3 days. Apoptosis induced by M-ds-P21 and ds-P21 treatment is not P53-dependent but caspase-dependent. Compared with ds-P21, M-ds-P21 significantly increased the bioavailability of ds-RNA in T24 cells.

Conclusions: M-ds-P21 treatment induces more apoptotic population than ds-P21 does. The mechanism for stronger effect of M-ds-P21 is partly due to the enhanced bioavailability of ds-RNA in human bladder cancer T24 cells, and not P53-dependent but caspase-dependent.

语种: 英语
所属项目编号: 81172419 ; 81170700
项目资助者: National Nature Science Foundation of China
WOS记录号: WOS:000321897000012
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/67486
Appears in Collections:北京大学第一临床医学院_泌尿外科_期刊论文

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作者单位: 1.Peking Univ, Dept Urol, Hosp 1, Beijing 100871, Peoples R China
2.Peking Univ, Inst Urol, Natl Res Ctr Genitourinary Oncol, Beijing 100871, Peoples R China

Recommended Citation:
Wang, Haifeng,Liu, Wujiang,Jin, Jie,et al. M-ds-P21 induces cell apoptosis in bladder cancer T24 cells through P53 independent pathway[J]. JOURNAL OF CANCER RESEARCH AND THERAPEUTICS,2013,9(1):54-59.
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