北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 北京大学第一临床医学院  > 泌尿外科  > 期刊论文
学科主题: 临床医学
题名:
M-ds-P21 induces cell apoptosis in bladder cancer T24 cells through P53 independent pathway
作者: Wang, Haifeng; Liu, Wujiang1,2; Jin, Jie; Zhou, Liqun; Liang, Lili; Guo, Yinglu
关键词: 2 &prime ; -Fluoride modification of ds-P21 ; apoptosis ; bladder cancer cells ; ds-P21 ; T24
刊名: JOURNAL OF CANCER RESEARCH AND THERAPEUTICS
发表日期: 2013
DOI: 10.4103/0973-1482.110367
卷: 9, 期:1, 页:54-59
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Oncology
研究领域[WOS]: Oncology
关键词[WOS]: RNA ; COMPLEX ; PHOSPHORYLATION ; ACTIVATION ; DUPLEXES ; SIRNAS ; DEATH ; P21
英文摘要:

Objectives: To investigate the effect of M-ds-P21 on the apoptosis of bladder cancer T24 cells and its potential mechanism.

Materials and Methods: Effect of M-ds-P21 on T24 cells were assessed by cell morphology and Western blot. Apoptosis was quantified by Annexin-V flow-cytometry analysis. To uncover the role of P53 in M-ds-P21-mediated apoptosis of T24 cells, we knocked down P53 before treating cells with M-ds-P21, and then assayed P21 and apoptosis-related protein by Western blot. To uncover the mechanism by which M-ds-P21 played stronger effect than ds-P21, we performed confocal microscope analyses.

Results: Both M-ds-P21 and ds-P21 treatment changed the cell morphology, leading to cell apoptosis after 3 days. Apoptosis induced by M-ds-P21 and ds-P21 treatment is not P53-dependent but caspase-dependent. Compared with ds-P21, M-ds-P21 significantly increased the bioavailability of ds-RNA in T24 cells.

Conclusions: M-ds-P21 treatment induces more apoptotic population than ds-P21 does. The mechanism for stronger effect of M-ds-P21 is partly due to the enhanced bioavailability of ds-RNA in human bladder cancer T24 cells, and not P53-dependent but caspase-dependent.

语种: 英语
所属项目编号: 81172419 ; 81170700
项目资助者: National Nature Science Foundation of China
WOS记录号: WOS:000321897000012
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/67486
Appears in Collections:北京大学第一临床医学院_泌尿外科_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Peking Univ, Dept Urol, Hosp 1, Beijing 100871, Peoples R China
2.Peking Univ, Inst Urol, Natl Res Ctr Genitourinary Oncol, Beijing 100871, Peoples R China

Recommended Citation:
Wang, Haifeng,Liu, Wujiang,Jin, Jie,et al. M-ds-P21 induces cell apoptosis in bladder cancer T24 cells through P53 independent pathway[J]. JOURNAL OF CANCER RESEARCH AND THERAPEUTICS,2013,9(1):54-59.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Wang, Haifeng]'s Articles
[Liu, Wujiang]'s Articles
[Jin, Jie]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Wang, Haifeng]‘s Articles
[Liu, Wujiang]‘s Articles
[Jin, Jie]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace