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学科主题: 临床医学
题名:
Bortezomib induces apoptosis and autophagy in osteosarcoma cells through mitogen-activated protein kinase pathway in vitro
作者: Lou, Zhiyuan; Ren, Tingting; Peng, Xianbo; Sun, Yifeng; Jiao, Guangjun; Lu, Qunshan; Zhang, Shuai; Lu, Xinchang; Guo, Wei
关键词: Apoptosis ; autophagy ; bortezomib ; mitogen-activated protein kinase (MAPK) ; cell-cycle arrest ; osteosarcoma ; proteasome inhibition ; transmission electron microscopy (TEM)
刊名: JOURNAL OF INTERNATIONAL MEDICAL RESEARCH
发表日期: 2013-10-01
DOI: 10.1177/0300060513490618
卷: 41, 期:5, 页:1505-1519
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Medicine, Research & Experimental ; Pharmacology & Pharmacy
研究领域[WOS]: Research & Experimental Medicine ; Pharmacology & Pharmacy
关键词[WOS]: PROTEASOME INHIBITOR BORTEZOMIB ; LEUKEMIA-CELLS ; CANCER-CELLS ; CYCLE ARREST ; RESISTANCE ; THERAPY ; FUTURE ; GROWTH
英文摘要:

Objective To investigate the effects of bortezomib on human osteosarcoma cells from the HOS cell line, and the underlying associated mechanisms.

Methods Viability of HOS cells treated with bortezomib (5-20nM) for different time periods was measured and changes in the cell cycle were assessed. Apoptosis and autophagy in HOS cells treated with bortezomib were analysed using annexin V-fluorescein isothiocyanate assay, transmission electron microscopy and Western blotting. Surges in mitogen-activated protein kinase (MAPK) pathways including MAPK/extracellular signal-regulated kinase (ERK) kinase (MEK1/2), ERK1/2, c-Jun N-terminal kinase (JNK) and p38 MAPK were analysed using Western blotting.

Results Bortezomib induced growth inhibition in a time- and dose-dependent manner, and autophagy and apoptosis in a dose-dependent manner, in HOS cells. HOS cell autophagy and apoptosis in response to bortezomib, corresponded with changing levels of intracellular MAPK signalling molecules.

Conclusions This study provided new insights into the mechanisms underlying bortezomib-induced apoptosis in human osteosarcoma HOS cells, and suggests that bortezomib could be a potent chemotherapeutic agent in the treatment of osteosarcoma.

语种: 英语
所属项目编号: 81172543 ; 81001193
项目资助者: National Natural Sciences Foundation of China
WOS记录号: WOS:000325273700013
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/67487
Appears in Collections:北京大学第二临床医学院_期刊论文

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作者单位: Peking Univ, Peoples Hosp, Musculoskeletal Tumour Ctr, Beijing 100044, Peoples R China

Recommended Citation:
Lou, Zhiyuan,Ren, Tingting,Peng, Xianbo,et al. Bortezomib induces apoptosis and autophagy in osteosarcoma cells through mitogen-activated protein kinase pathway in vitro[J]. JOURNAL OF INTERNATIONAL MEDICAL RESEARCH,2013,41(5):1505-1519.
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