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Bortezomib induces apoptosis and autophagy in osteosarcoma cells through mitogen-activated protein kinase pathway in vitro
Lou, Zhiyuan; Ren, Tingting; Peng, Xianbo; Sun, Yifeng; Jiao, Guangjun; Lu, Qunshan; Zhang, Shuai; Lu, Xinchang; Guo, Wei
关键词Apoptosis autophagy bortezomib mitogen-activated protein kinase (MAPK) cell-cycle arrest osteosarcoma proteasome inhibition transmission electron microscopy (TEM)
刊名JOURNAL OF INTERNATIONAL MEDICAL RESEARCH
2013-10-01
DOI10.1177/0300060513490618
41期:5页:1505-1519
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Medicine, Research & Experimental ; Pharmacology & Pharmacy
研究领域[WOS]Research & Experimental Medicine ; Pharmacology & Pharmacy
关键词[WOS]PROTEASOME INHIBITOR BORTEZOMIB ; LEUKEMIA-CELLS ; CANCER-CELLS ; CYCLE ARREST ; RESISTANCE ; THERAPY ; FUTURE ; GROWTH
英文摘要

Objective To investigate the effects of bortezomib on human osteosarcoma cells from the HOS cell line, and the underlying associated mechanisms.

Methods Viability of HOS cells treated with bortezomib (5-20nM) for different time periods was measured and changes in the cell cycle were assessed. Apoptosis and autophagy in HOS cells treated with bortezomib were analysed using annexin V-fluorescein isothiocyanate assay, transmission electron microscopy and Western blotting. Surges in mitogen-activated protein kinase (MAPK) pathways including MAPK/extracellular signal-regulated kinase (ERK) kinase (MEK1/2), ERK1/2, c-Jun N-terminal kinase (JNK) and p38 MAPK were analysed using Western blotting.

Results Bortezomib induced growth inhibition in a time- and dose-dependent manner, and autophagy and apoptosis in a dose-dependent manner, in HOS cells. HOS cell autophagy and apoptosis in response to bortezomib, corresponded with changing levels of intracellular MAPK signalling molecules.

Conclusions This study provided new insights into the mechanisms underlying bortezomib-induced apoptosis in human osteosarcoma HOS cells, and suggests that bortezomib could be a potent chemotherapeutic agent in the treatment of osteosarcoma.

语种英语
WOS记录号WOS:000325273700013
项目编号81172543 ; 81001193
资助机构National Natural Sciences Foundation of China
引用统计
被引频次:20[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/67487
专题北京大学第二临床医学院
北京大学药学院_天然药物与仿生药物国家重点实验室
作者单位Peking Univ, Peoples Hosp, Musculoskeletal Tumour Ctr, Beijing 100044, Peoples R China
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Lou, Zhiyuan,Ren, Tingting,Peng, Xianbo,et al. Bortezomib induces apoptosis and autophagy in osteosarcoma cells through mitogen-activated protein kinase pathway in vitro[J]. JOURNAL OF INTERNATIONAL MEDICAL RESEARCH,2013,41(5):1505-1519.
APA Lou, Zhiyuan.,Ren, Tingting.,Peng, Xianbo.,Sun, Yifeng.,Jiao, Guangjun.,...&Guo, Wei.(2013).Bortezomib induces apoptosis and autophagy in osteosarcoma cells through mitogen-activated protein kinase pathway in vitro.JOURNAL OF INTERNATIONAL MEDICAL RESEARCH,41(5),1505-1519.
MLA Lou, Zhiyuan,et al."Bortezomib induces apoptosis and autophagy in osteosarcoma cells through mitogen-activated protein kinase pathway in vitro".JOURNAL OF INTERNATIONAL MEDICAL RESEARCH 41.5(2013):1505-1519.
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