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学科主题临床医学
Simultaneous determination of 15 steroids in rat blood via gas chromatography-mass spectrometry to evaluate the impact of emasculation on adrenal
Chen, Jun1; Liang, Qionglin1; Hua, Haiyin1; Wang, Yiming1; Luo, Guo′ an1; Hu, Mingqiu2; Na, Yanqun2
关键词Adrenal Emasculation Steroids Gas chromatography Mass spectrum
刊名TALANTA
2009-12-15
DOI10.1016/j.talanta.2009.07.066
80期:2页:826-832
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Chemistry, Analytical
研究领域[WOS]Chemistry
关键词[WOS]PERFORMANCE LIQUID-CHROMATOGRAPHY ; ANABOLIC-STEROIDS ; PROSTATE-CANCER ; GC-MS ; URINE ; TESTOSTERONE ; VALIDATION ; DEHYDROEPIANDROSTERONE ; EXPRESSION ; ANDROGENS
英文摘要

Adrenal was believed to affect the prostate tumor tissue growth by its secretion of adrenal androgens. However, the mechanisms regulating these effects were not fully understood. In this work, a sensitive and specific method for the determination of 15 steroids in blood via gas chromatography-mass spectrometry in selective ion storage detection mode was established to evaluate the impact of emasculation on adrenal steroids metabolism. Steroids were isolated by solid-phase extraction using Oasis HLB cartridge, and then derivated with heptafluorobutyric anhydride before analysis. The limits of detection were between 0.15 and 1.0 ng/mL and limits of quantification were between 0.62 and 2.6 ng/mL The recoveries of steroids were above 83%, and both the intra-day and inter-day precisions (RSD%) were lower than 8%. Pregnenolone, progesterone, 17 alpha-hydroxyprogesterone (17 alpha P), 17 alpha-hydroxypregnenolone (17 alpha H), dehydroepiandrosterone (DHEA), estrone, 17 beta-estradiol, dihydrotestosterone (DHT), testosterone (T), 4-androstenedione (4-A), 1,4-androstadiene-3,17-dione, 11-deoxycortisol, 11-deoxycorticosterone, cortisol and aldosterone were quantified in 156 major male SD rats at 0, 1, 2,4, 7 days, 2, 4, 6, 8,10,12, 14, and 16 weeks following emasculation. T and DHT decreased by 86.2% and 73.4%, respectively in the first 7 days following emasculation, but adrenal androgens (DHEA, 4-A) stabled at the normal level accordingly. Adrenal androgens and their precursors (17 alpha H, 17 alpha P) increased from the 2nd week along with the increase of androgens and the decrease of mineralocorticoids. These facts revealed that adrenal possibly enhanced its function of producing adrenal androgens from the 2nd week responding to the low androgens level induced by emasculation. (C) 2009 Elsevier B.V. All rights reserved.

语种英语
WOS记录号WOS:000271701900063
项目编号30772169 ; 20805026 ; 90709045 ; 2005CB523503 ; 2007CB714505 ; 2007CB511903
资助机构National Natural Science Foundation of China ; National Basic Research Program (973 Program) of China
引用统计
被引频次:11[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/67491
专题北京大学第一临床医学院_泌尿外科
作者单位1.Tsinghua Univ, Dept Chem, Key Lab Bioorgan Phosphorus Chem & Chem Biol, Minist Educ, Beijing 100084, Peoples R China
2.Peking Univ, Hosp 1, Dept Urol, Inst Urol, Beijing 100034, Peoples R China
推荐引用方式
GB/T 7714
Chen, Jun,Liang, Qionglin,Hua, Haiyin,et al. Simultaneous determination of 15 steroids in rat blood via gas chromatography-mass spectrometry to evaluate the impact of emasculation on adrenal[J]. TALANTA,2009,80(2):826-832.
APA Chen, Jun.,Liang, Qionglin.,Hua, Haiyin.,Wang, Yiming.,Luo, Guo&prime.,...&Na, Yanqun.(2009).Simultaneous determination of 15 steroids in rat blood via gas chromatography-mass spectrometry to evaluate the impact of emasculation on adrenal.TALANTA,80(2),826-832.
MLA Chen, Jun,et al."Simultaneous determination of 15 steroids in rat blood via gas chromatography-mass spectrometry to evaluate the impact of emasculation on adrenal".TALANTA 80.2(2009):826-832.
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